Using of Microsatellites to Study the Genetic Polymorphisms of SRYm18 Region in Iraqi Sheep
The origin of animals is usually determined by their paternal genotypes of the genes on the Y chromosome. In addition to the genes and their polymorphisms in the genome of mitochondria that are inherited through dams. In view of the lack of studies focusing on the genes of the Y chromosome in...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Diyala
2021-12-01
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| Series: | مجلة ديالى للعلوم الزراعية |
| Subjects: | |
| Online Access: | https://journal.djas.uodiyala.edu.iq/index.php/dasj/article/view/2107 |
| Summary: | The origin of animals is usually determined by their paternal genotypes of the
genes on the Y chromosome. In addition to the genes and their polymorphisms
in the genome of mitochondria that are inherited through dams. In view of the
lack of studies focusing on the genes of the Y chromosome in the world and
their absence in Iraq. The aim of the present study was to identify the multiple
genetic polymorphisms of the SRYM18 gene in the Arabi and Awassi sheep
raised in Iraq. The study was conducted in the Genetic Engineering
Laboratories - College of Agriculture, University of Basra, as well as in the
Basra Genome Laboratory. The amplification of the SRYM18 gene showed
genetic polymorphisms and gave a gene segment of (103-880) bp. The
number of alleles of the SRYm18 gene was 13 alleles in the Arabi and 16
alleles in the Awassi breeds. The equilibrium test showed that the two breeds
were under equilibrium. The two breeds were identical with nine alleles, while
the number of special alleles for the Arabi breed was two, while the Awassi
breed was distinguished by five. The number of rare alleles reached 20, of
which seven were of the Arabi breed, and 13 of them were of the Awassi
breed. Mean expected heterozygosity was 0.6386 with nonsignificant Fis for
Arabi breed (0.1541) but significant for Awassi breed (0.2213). Mean
neutrality was close to lower bound (0.1721) and (0.1270) for Arabi and
Awassi breeds respectively |
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| ISSN: | 2073-9524 2310-8746 |