The Ethanolic Extract of <i>Lycium ruthenicum</i> Ameliorates Age-Related Physiological Damage in Mice
Aging and age-related diseases are important study topics due to their associations with progressive physiological damage to genes, cells, tissues, and the entire organism, which ultimately affects the functional efficiency of organs. <i>Lycium ruthenicum</i> Murr. is a functional food t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/22/7615 |
| _version_ | 1797458265491111936 |
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| author | Boya Cui Lanying Liu Tao Shi Min Yin Xu Feng Yu Shan |
| author_facet | Boya Cui Lanying Liu Tao Shi Min Yin Xu Feng Yu Shan |
| author_sort | Boya Cui |
| collection | DOAJ |
| description | Aging and age-related diseases are important study topics due to their associations with progressive physiological damage to genes, cells, tissues, and the entire organism, which ultimately affects the functional efficiency of organs. <i>Lycium ruthenicum</i> Murr. is a functional food that is known for its high contents of anthocyanins and spermidines, both of which have been demonstrated to have positive effects on anti-aging activity and anti-oxidation. In this study, we used HPLC-MS to analyze the constituents of <i>L. ruthenicum</i> Murr. Extract (LRM) and investigated their potential mechanism for exerting antioxidative effects in D-galactose (D-Gal) aging model mice. LRM (25 mg/kg, 50 mg/kg, and 100 mg/kg) improved cognitive function in D-Gal-treated mice, as shown by reduced escape latencies and increased platform crossings in behavioral tests. We measured the contents of lipid peroxidation (LPO) and malondialdehyde (MDA) and the enzyme activities of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in mice serum and brain after 6 weeks of D-Gal treatment. LRM decreased the contents of LPO and MDA and increased the enzyme activities of SOD and GSH-Px, indicating the protection effect of LRM against D-Gal-induced oxidative stress. Additionally, LRM can inhibit oxidative stress in cells by reducing intracellular ROS levels and restoring mitochondrial membrane potential, thereby inhibiting paraquat (PQ)-induced cellular senescence and delaying cell aging. Therefore, LRM has the potential to be a healthcare product for the treatment of age-related diseases. |
| first_indexed | 2024-03-09T16:34:36Z |
| format | Article |
| id | doaj.art-b81149a1d040428fbabc927f0aa2b506 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:34:36Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b81149a1d040428fbabc927f0aa2b5062023-11-24T14:58:26ZengMDPI AGMolecules1420-30492023-11-012822761510.3390/molecules28227615The Ethanolic Extract of <i>Lycium ruthenicum</i> Ameliorates Age-Related Physiological Damage in MiceBoya Cui0Lanying Liu1Tao Shi2Min Yin3Xu Feng4Yu Shan5Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, Nanjing 210014, ChinaNational Wolfberry Engineering Research Center, Institute of Wolfberry Engineering Technology, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, Nanjing 210014, ChinaAging and age-related diseases are important study topics due to their associations with progressive physiological damage to genes, cells, tissues, and the entire organism, which ultimately affects the functional efficiency of organs. <i>Lycium ruthenicum</i> Murr. is a functional food that is known for its high contents of anthocyanins and spermidines, both of which have been demonstrated to have positive effects on anti-aging activity and anti-oxidation. In this study, we used HPLC-MS to analyze the constituents of <i>L. ruthenicum</i> Murr. Extract (LRM) and investigated their potential mechanism for exerting antioxidative effects in D-galactose (D-Gal) aging model mice. LRM (25 mg/kg, 50 mg/kg, and 100 mg/kg) improved cognitive function in D-Gal-treated mice, as shown by reduced escape latencies and increased platform crossings in behavioral tests. We measured the contents of lipid peroxidation (LPO) and malondialdehyde (MDA) and the enzyme activities of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in mice serum and brain after 6 weeks of D-Gal treatment. LRM decreased the contents of LPO and MDA and increased the enzyme activities of SOD and GSH-Px, indicating the protection effect of LRM against D-Gal-induced oxidative stress. Additionally, LRM can inhibit oxidative stress in cells by reducing intracellular ROS levels and restoring mitochondrial membrane potential, thereby inhibiting paraquat (PQ)-induced cellular senescence and delaying cell aging. Therefore, LRM has the potential to be a healthcare product for the treatment of age-related diseases.https://www.mdpi.com/1420-3049/28/22/7615anthocyaninsspermidinesoxidative stresscognitive impairmentcellular senescence |
| spellingShingle | Boya Cui Lanying Liu Tao Shi Min Yin Xu Feng Yu Shan The Ethanolic Extract of <i>Lycium ruthenicum</i> Ameliorates Age-Related Physiological Damage in Mice Molecules anthocyanins spermidines oxidative stress cognitive impairment cellular senescence |
| title | The Ethanolic Extract of <i>Lycium ruthenicum</i> Ameliorates Age-Related Physiological Damage in Mice |
| title_full | The Ethanolic Extract of <i>Lycium ruthenicum</i> Ameliorates Age-Related Physiological Damage in Mice |
| title_fullStr | The Ethanolic Extract of <i>Lycium ruthenicum</i> Ameliorates Age-Related Physiological Damage in Mice |
| title_full_unstemmed | The Ethanolic Extract of <i>Lycium ruthenicum</i> Ameliorates Age-Related Physiological Damage in Mice |
| title_short | The Ethanolic Extract of <i>Lycium ruthenicum</i> Ameliorates Age-Related Physiological Damage in Mice |
| title_sort | ethanolic extract of i lycium ruthenicum i ameliorates age related physiological damage in mice |
| topic | anthocyanins spermidines oxidative stress cognitive impairment cellular senescence |
| url | https://www.mdpi.com/1420-3049/28/22/7615 |
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