Molecular cloning and expression levels of the monoterpene synthase gene (ZMM1) in Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.)
Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) is a native Thai herb with a high content and large variety of terpenoids in its essential oil. Improving the essential oil content and quality of cassumunar ginger is difficult for a breeder due to its clonally propagated nature...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2014-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641404321B.pdf |
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| author | Bua-In Saowaluck Paisooksantivatana Yingyong Weimer Bart C. Chowpongpang Srimek |
| author_facet | Bua-In Saowaluck Paisooksantivatana Yingyong Weimer Bart C. Chowpongpang Srimek |
| author_sort | Bua-In Saowaluck |
| collection | DOAJ |
| description | Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) is a native
Thai herb with a high content and large variety of terpenoids in its
essential oil. Improving the essential oil content and quality of cassumunar
ginger is difficult for a breeder due to its clonally propagated nature. In
this research, we describe the isolation and expression level of the
monoterpene synthase gene that controls the key step of essential oil
synthesis in this plant and evaluate the mechanical wounding that may
influence the transcription level of the monoterpene synthase gene. To
isolate the gene, the selected clones from DNA derived from young leaves were
sequenced and analyzed and the monoterpene synthase gene from cassumunar
ginger (ZMM1) was identified. The ZMM1 CDS containing 1 773 bp (KF500399) is
predicted to encode a protein of 590 amino acids. The deduced amino acid
sequence is 40-74% identical with known sequences of other angiosperm
monoterpene synthases belonging to the isoprenoid biosynthesis C1
superfamily. A transcript of ZMM1 was detected almost exclusively in the
leaves and was related to leaf wounding. The results of this research offer
insight into the control of monoterpene synthesis in this plant. This finding
can be applied to breeding programs or crop management of cassumunar ginger
for better yield and quality of essential oil. |
| first_indexed | 2024-12-11T05:56:36Z |
| format | Article |
| id | doaj.art-b8148e85dc6344eabbb9d25c5325acee |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-11T05:56:36Z |
| publishDate | 2014-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-b8148e85dc6344eabbb9d25c5325acee2022-12-22T01:18:39ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392014-01-016641321133110.2298/ABS1404321B0354-46641404321BMolecular cloning and expression levels of the monoterpene synthase gene (ZMM1) in Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.)Bua-In Saowaluck0Paisooksantivatana Yingyong1Weimer Bart C.2Chowpongpang Srimek3Kasetsart University, Faculty of Agriculture, Department of Horticulture, Bangkok, ThailandKasetsart University, Faculty of Agriculture, Department of Horticulture, Bangkok, ThailandUniversity of California, School of Veterinary Medicine, Population Health & Reproduction, Davis, USAKasetsart University, Faculty of Agriculture, Department of Plant Pathology, Bangkok, ThailandCassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) is a native Thai herb with a high content and large variety of terpenoids in its essential oil. Improving the essential oil content and quality of cassumunar ginger is difficult for a breeder due to its clonally propagated nature. In this research, we describe the isolation and expression level of the monoterpene synthase gene that controls the key step of essential oil synthesis in this plant and evaluate the mechanical wounding that may influence the transcription level of the monoterpene synthase gene. To isolate the gene, the selected clones from DNA derived from young leaves were sequenced and analyzed and the monoterpene synthase gene from cassumunar ginger (ZMM1) was identified. The ZMM1 CDS containing 1 773 bp (KF500399) is predicted to encode a protein of 590 amino acids. The deduced amino acid sequence is 40-74% identical with known sequences of other angiosperm monoterpene synthases belonging to the isoprenoid biosynthesis C1 superfamily. A transcript of ZMM1 was detected almost exclusively in the leaves and was related to leaf wounding. The results of this research offer insight into the control of monoterpene synthesis in this plant. This finding can be applied to breeding programs or crop management of cassumunar ginger for better yield and quality of essential oil.http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641404321B.pdfgene isolationterpene synthasetranscriptleafterpinen-4-ol |
| spellingShingle | Bua-In Saowaluck Paisooksantivatana Yingyong Weimer Bart C. Chowpongpang Srimek Molecular cloning and expression levels of the monoterpene synthase gene (ZMM1) in Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) Archives of Biological Sciences gene isolation terpene synthase transcript leaf terpinen-4-ol |
| title | Molecular cloning and expression levels of the monoterpene synthase gene (ZMM1) in Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) |
| title_full | Molecular cloning and expression levels of the monoterpene synthase gene (ZMM1) in Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) |
| title_fullStr | Molecular cloning and expression levels of the monoterpene synthase gene (ZMM1) in Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) |
| title_full_unstemmed | Molecular cloning and expression levels of the monoterpene synthase gene (ZMM1) in Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) |
| title_short | Molecular cloning and expression levels of the monoterpene synthase gene (ZMM1) in Cassumunar ginger (Zingiber montanum (Koenig) Link ex Dietr.) |
| title_sort | molecular cloning and expression levels of the monoterpene synthase gene zmm1 in cassumunar ginger zingiber montanum koenig link ex dietr |
| topic | gene isolation terpene synthase transcript leaf terpinen-4-ol |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641404321B.pdf |
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