MicroRNA‐324‐5p affects the radiotherapy response of cervical cancer via targeting ELAV‐like RNA binding protein 1
Abstract Cervical cancer (CC) seriously threatens the health of women. Radiation therapy (RT) is the major treatment for CC. However, the recurrent CC can acquire resistance to RT. Thus, it is necessary to find a new method for reversing RT resistance in CC. It has been reported that miR‐324‐5p can...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-12-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12277 |
| _version_ | 1797237780759183360 |
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| author | Ming‐Jun Fan Peng‐Juan He Xue‐Yan Lin Chun‐Run Yang Chang‐Zhong Li Li‐Gang Xing |
| author_facet | Ming‐Jun Fan Peng‐Juan He Xue‐Yan Lin Chun‐Run Yang Chang‐Zhong Li Li‐Gang Xing |
| author_sort | Ming‐Jun Fan |
| collection | DOAJ |
| description | Abstract Cervical cancer (CC) seriously threatens the health of women. Radiation therapy (RT) is the major treatment for CC. However, the recurrent CC can acquire resistance to RT. Thus, it is necessary to find a new method for reversing RT resistance in CC. It has been reported that miR‐324‐5p can suppress the progression of multiple cancers. However, whether it can reverse resistance to RT in CC remains unclear. qRT‐PCR and Western blotting were used to detect gene and protein expression in CC cells, respectively. Cell proliferation was tested by CCK‐8 assay and colony formation assay. In addition, cell apoptosis was detected by flow cytometry. Transwell assays were performed to detect cell migration. Dual luciferase reporter assay and TargetScan were used to explore the targets of microRNA‐324‐5p (miR‐324‐5p). MiR‐324‐5p was downregulated in CC cells. Overexpression of miR‐324‐5p sensitized CC cells to RT. In addition, miR‐324‐5p mimics significantly induced apoptosis and inhibits the migration of CC cells in the presence of 137Cs ionizing radiation. Furthermore, miR‐324‐5p sensitized CC cells to ionizing radiation by targeting ELAV‐like RNA binding protein 1 (ELAVL1). MiR‐324‐5p overexpression affects the radiotherapy response of CC by targeting ELAVL1, which may serve as a new target for the treatment of CC. |
| first_indexed | 2024-04-24T17:25:11Z |
| format | Article |
| id | doaj.art-b817628bd35f47af8b4c43472bfef556 |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-04-24T17:25:11Z |
| publishDate | 2020-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-b817628bd35f47af8b4c43472bfef5562024-03-28T07:06:00ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502020-12-01361296597210.1002/kjm2.12277MicroRNA‐324‐5p affects the radiotherapy response of cervical cancer via targeting ELAV‐like RNA binding protein 1Ming‐Jun Fan0Peng‐Juan He1Xue‐Yan Lin2Chun‐Run Yang3Chang‐Zhong Li4Li‐Gang Xing5Department of Radiation Oncology Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences Jinan Shandong ChinaDepartment of Obstetrics and Gynecology Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan Shandong ChinaDepartment of Obstetrics and Gynecology Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan Shandong ChinaDepartment of Obstetrics and Gynecology Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan Shandong ChinaPostdoctoral Mobile Station of Shandong University of Traditional Chinese Medicine Jinan Shandong ChinaDepartment of Radiation Oncology Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences Jinan Shandong ChinaAbstract Cervical cancer (CC) seriously threatens the health of women. Radiation therapy (RT) is the major treatment for CC. However, the recurrent CC can acquire resistance to RT. Thus, it is necessary to find a new method for reversing RT resistance in CC. It has been reported that miR‐324‐5p can suppress the progression of multiple cancers. However, whether it can reverse resistance to RT in CC remains unclear. qRT‐PCR and Western blotting were used to detect gene and protein expression in CC cells, respectively. Cell proliferation was tested by CCK‐8 assay and colony formation assay. In addition, cell apoptosis was detected by flow cytometry. Transwell assays were performed to detect cell migration. Dual luciferase reporter assay and TargetScan were used to explore the targets of microRNA‐324‐5p (miR‐324‐5p). MiR‐324‐5p was downregulated in CC cells. Overexpression of miR‐324‐5p sensitized CC cells to RT. In addition, miR‐324‐5p mimics significantly induced apoptosis and inhibits the migration of CC cells in the presence of 137Cs ionizing radiation. Furthermore, miR‐324‐5p sensitized CC cells to ionizing radiation by targeting ELAV‐like RNA binding protein 1 (ELAVL1). MiR‐324‐5p overexpression affects the radiotherapy response of CC by targeting ELAVL1, which may serve as a new target for the treatment of CC.https://doi.org/10.1002/kjm2.12277apoptosiscervical cancerELAVL1miR‐324‐5pradiation therapy |
| spellingShingle | Ming‐Jun Fan Peng‐Juan He Xue‐Yan Lin Chun‐Run Yang Chang‐Zhong Li Li‐Gang Xing MicroRNA‐324‐5p affects the radiotherapy response of cervical cancer via targeting ELAV‐like RNA binding protein 1 Kaohsiung Journal of Medical Sciences apoptosis cervical cancer ELAVL1 miR‐324‐5p radiation therapy |
| title | MicroRNA‐324‐5p affects the radiotherapy response of cervical cancer via targeting ELAV‐like RNA binding protein 1 |
| title_full | MicroRNA‐324‐5p affects the radiotherapy response of cervical cancer via targeting ELAV‐like RNA binding protein 1 |
| title_fullStr | MicroRNA‐324‐5p affects the radiotherapy response of cervical cancer via targeting ELAV‐like RNA binding protein 1 |
| title_full_unstemmed | MicroRNA‐324‐5p affects the radiotherapy response of cervical cancer via targeting ELAV‐like RNA binding protein 1 |
| title_short | MicroRNA‐324‐5p affects the radiotherapy response of cervical cancer via targeting ELAV‐like RNA binding protein 1 |
| title_sort | microrna 324 5p affects the radiotherapy response of cervical cancer via targeting elav like rna binding protein 1 |
| topic | apoptosis cervical cancer ELAVL1 miR‐324‐5p radiation therapy |
| url | https://doi.org/10.1002/kjm2.12277 |
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