Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications
Avian models are valuable for studies of development and reproduction and have important implications for food production. Rapid advances in genome-editing technologies have enabled the establishment of avian species as unique agricultural, industrial, disease-resistant, and pharmaceutical models. T...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/4/899 |
| _version_ | 1797605317126651904 |
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| author | Young-Min Kim Seung-Je Woo Jae-Yong Han |
| author_facet | Young-Min Kim Seung-Je Woo Jae-Yong Han |
| author_sort | Young-Min Kim |
| collection | DOAJ |
| description | Avian models are valuable for studies of development and reproduction and have important implications for food production. Rapid advances in genome-editing technologies have enabled the establishment of avian species as unique agricultural, industrial, disease-resistant, and pharmaceutical models. The direct introduction of genome-editing tools, such as the clustered regularly interspaced short palindromic repeats (CRISPR) system, into early embryos has been achieved in various animal taxa. However, in birds, the introduction of the CRISPR system into primordial germ cells (PGCs), a germline-competent stem cell, is considered a much more reliable approach for the development of genome-edited models. After genome editing, PGCs are transplanted into the embryo to establish germline chimera, which are crossed to produce genome-edited birds. In addition, various methods, including delivery by liposomal and viral vectors, have been employed for gene editing in vivo. Genome-edited birds have wide applications in bio-pharmaceutical production and as models for disease resistance and biological research. In conclusion, the application of the CRISPR system to avian PGCs is an efficient approach for the production of genome-edited birds and transgenic avian models. |
| first_indexed | 2024-03-11T04:59:20Z |
| format | Article |
| id | doaj.art-b818e5a6181a4e9fb41ab24bcee5ee34 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T04:59:20Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-b818e5a6181a4e9fb41ab24bcee5ee342023-11-17T19:24:09ZengMDPI AGGenes2073-44252023-04-0114489910.3390/genes14040899Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and ApplicationsYoung-Min Kim0Seung-Je Woo1Jae-Yong Han2Avinnogen Co., Ltd., Seoul 08826, Republic of KoreaDepartment of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of KoreaAvinnogen Co., Ltd., Seoul 08826, Republic of KoreaAvian models are valuable for studies of development and reproduction and have important implications for food production. Rapid advances in genome-editing technologies have enabled the establishment of avian species as unique agricultural, industrial, disease-resistant, and pharmaceutical models. The direct introduction of genome-editing tools, such as the clustered regularly interspaced short palindromic repeats (CRISPR) system, into early embryos has been achieved in various animal taxa. However, in birds, the introduction of the CRISPR system into primordial germ cells (PGCs), a germline-competent stem cell, is considered a much more reliable approach for the development of genome-edited models. After genome editing, PGCs are transplanted into the embryo to establish germline chimera, which are crossed to produce genome-edited birds. In addition, various methods, including delivery by liposomal and viral vectors, have been employed for gene editing in vivo. Genome-edited birds have wide applications in bio-pharmaceutical production and as models for disease resistance and biological research. In conclusion, the application of the CRISPR system to avian PGCs is an efficient approach for the production of genome-edited birds and transgenic avian models.https://www.mdpi.com/2073-4425/14/4/899avian modelgenome editingprimordial germ cell (PGC)CRISPR/Cas9 |
| spellingShingle | Young-Min Kim Seung-Je Woo Jae-Yong Han Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications Genes avian model genome editing primordial germ cell (PGC) CRISPR/Cas9 |
| title | Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications |
| title_full | Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications |
| title_fullStr | Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications |
| title_full_unstemmed | Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications |
| title_short | Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications |
| title_sort | strategies for the generation of gene modified avian models advancement in avian germline transmission genome editing and applications |
| topic | avian model genome editing primordial germ cell (PGC) CRISPR/Cas9 |
| url | https://www.mdpi.com/2073-4425/14/4/899 |
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