Ensiling process and pomegranate peel extract as a natural additive in potential prevention of fungal and mycotoxin contamination in silage
A study was conducted on six animal feed centers in Israel where fungal and mycotoxin presence was examined in maize and wheat silages. Fumonisin mycotoxins FB1 and FB2 were present in every maize silage sample analyzed. Interestingly, no correlation was found between the occurrence of specific myco...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750022001652 |
| _version_ | 1811295190258810880 |
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| author | Sudharsan Sadhasivam Rula Marshi Omer Barda Varda Zakin Malka Britzi Abraham Gamliel Edward Sionov |
| author_facet | Sudharsan Sadhasivam Rula Marshi Omer Barda Varda Zakin Malka Britzi Abraham Gamliel Edward Sionov |
| author_sort | Sudharsan Sadhasivam |
| collection | DOAJ |
| description | A study was conducted on six animal feed centers in Israel where fungal and mycotoxin presence was examined in maize and wheat silages. Fumonisin mycotoxins FB1 and FB2 were present in every maize silage sample analyzed. Interestingly, no correlation was found between the occurrence of specific mycotoxins and the presence of the fungal species that might produce them in maize and wheat silages. We further investigated the effect of pomegranate peel extract (PPE) on Fusarium infection and fumonisin biosynthesis in laboratory-prepared maize silage. PPE had an inhibitory effect on FB1 and FB2 biosynthesis by Fusarium proliferatum, which resulted in up to 90 % reduction of fumonisin production in silage samples compared to untreated controls. This finding was supported by qRT-PCR analysis, showing downregulation of key genes involved in the fumonisin-biosynthesis pathway under PPE treatment. Our results present promising new options for the use of natural compounds that may help reduce fungal and mycotoxin contamination in agricultural foodstuff, and potentially replace traditionally used synthetic chemicals. |
| first_indexed | 2024-04-13T05:28:50Z |
| format | Article |
| id | doaj.art-b81ad3dd7c184aa0b0fb4e8e31eea5ad |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-04-13T05:28:50Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-b81ad3dd7c184aa0b0fb4e8e31eea5ad2022-12-22T03:00:30ZengElsevierToxicology Reports2214-75002022-01-01915571565Ensiling process and pomegranate peel extract as a natural additive in potential prevention of fungal and mycotoxin contamination in silageSudharsan Sadhasivam0Rula Marshi1Omer Barda2Varda Zakin3Malka Britzi4Abraham Gamliel5Edward Sionov6Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7528809, Israel; The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot 7610001, IsraelDepartment of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7528809, Israel; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, IsraelDepartment of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7528809, IsraelDepartment of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7528809, IsraelNational Residue Control Laboratory, Kimron Veterinary Institute, Rishon LeZion 7534503, IsraelDepartment of Growing, Production and Environmental Engineering, Institute of Agricultural Engineering, Agricultural Research Organization, Volcani Center, Rishon LeZion 7528809, IsraelDepartment of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7528809, Israel; Corresponding author.A study was conducted on six animal feed centers in Israel where fungal and mycotoxin presence was examined in maize and wheat silages. Fumonisin mycotoxins FB1 and FB2 were present in every maize silage sample analyzed. Interestingly, no correlation was found between the occurrence of specific mycotoxins and the presence of the fungal species that might produce them in maize and wheat silages. We further investigated the effect of pomegranate peel extract (PPE) on Fusarium infection and fumonisin biosynthesis in laboratory-prepared maize silage. PPE had an inhibitory effect on FB1 and FB2 biosynthesis by Fusarium proliferatum, which resulted in up to 90 % reduction of fumonisin production in silage samples compared to untreated controls. This finding was supported by qRT-PCR analysis, showing downregulation of key genes involved in the fumonisin-biosynthesis pathway under PPE treatment. Our results present promising new options for the use of natural compounds that may help reduce fungal and mycotoxin contamination in agricultural foodstuff, and potentially replace traditionally used synthetic chemicals.http://www.sciencedirect.com/science/article/pii/S2214750022001652SilageFungiMycotoxin analysisFumonisinsPomegranate peel extract |
| spellingShingle | Sudharsan Sadhasivam Rula Marshi Omer Barda Varda Zakin Malka Britzi Abraham Gamliel Edward Sionov Ensiling process and pomegranate peel extract as a natural additive in potential prevention of fungal and mycotoxin contamination in silage Toxicology Reports Silage Fungi Mycotoxin analysis Fumonisins Pomegranate peel extract |
| title | Ensiling process and pomegranate peel extract as a natural additive in potential prevention of fungal and mycotoxin contamination in silage |
| title_full | Ensiling process and pomegranate peel extract as a natural additive in potential prevention of fungal and mycotoxin contamination in silage |
| title_fullStr | Ensiling process and pomegranate peel extract as a natural additive in potential prevention of fungal and mycotoxin contamination in silage |
| title_full_unstemmed | Ensiling process and pomegranate peel extract as a natural additive in potential prevention of fungal and mycotoxin contamination in silage |
| title_short | Ensiling process and pomegranate peel extract as a natural additive in potential prevention of fungal and mycotoxin contamination in silage |
| title_sort | ensiling process and pomegranate peel extract as a natural additive in potential prevention of fungal and mycotoxin contamination in silage |
| topic | Silage Fungi Mycotoxin analysis Fumonisins Pomegranate peel extract |
| url | http://www.sciencedirect.com/science/article/pii/S2214750022001652 |
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