Upregulation of T-Cell-Specific Transcription Factor Expression in Pediatric T-Cell Acute Lymphoblastic Leukemia (T-ALL)
OBJECTIVE: T-cell acute lymphoblastic leukemia (T-ALL) is associated with recurrent chromosomal aberrations and abnormal ectopic gene expression during T-cell development. In order to gain insight into the pathogenesis of T-ALL this study aimed to measure the level of expression of 7 T-cell oncogene...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2012-12-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-13540 |
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| author | Müge Sayitoğlu Yücel Erbilgin Özden Hatırnaz İnci Yıldız Tiraje Celkan Sema Anak Ömer Devecioğlu Gönül Aydoğan Serap Karaman Nazan Sarper Çetin Timur Ümit Üre Uğur Özbek |
| author_facet | Müge Sayitoğlu Yücel Erbilgin Özden Hatırnaz İnci Yıldız Tiraje Celkan Sema Anak Ömer Devecioğlu Gönül Aydoğan Serap Karaman Nazan Sarper Çetin Timur Ümit Üre Uğur Özbek |
| author_sort | Müge Sayitoğlu |
| collection | DOAJ |
| description | OBJECTIVE: T-cell acute lymphoblastic leukemia (T-ALL) is associated with recurrent chromosomal aberrations and abnormal ectopic gene expression during T-cell development. In order to gain insight into the pathogenesis of T-ALL this study aimed to measure the level of expression of 7 T-cell oncogenes (LMO2, LYL1, TAL1, TLX1, TLX3, BMI1, and CALM-AF10) in pediatric T-ALL patients. METHODS: LMO2, LYL1, TLX1, TLX3, BMI1, TAL1, and CALM-AF10 expression was measured using quantitative real-time PCR in 43 pediatric T-ALL patients. RESULTS: A high level of expression of LMO2, LYL1, TAL1, and BMI1 genes was observed in a large group of T-ALL. Several gene expression signatures indicative of leukemic arrest at specific stages of normal thymocyte development (LYL1 and LMO2) were highly expressed during the cortical and mature stages of T-cell development. Furthermore, upregulated TAL1 and BMI1 expression was observed in all phenotypic subgroups. In all, 6 of the patients had TLX1 and TLX3 proto-oncogene expression, which does not occur in normal cells, and none of the patients had CALM-AF10 fusion gene transcription. Expression of LYL1 alone and LMO2-LYL1 co-expression were associated with mediastinal involvement; however, high-level oncogene expression was not predictive of outcome in the present pediatric T-ALL patient group, but there was a trend towards a poor prognostic impact of TAL1 and/or LMO2 and/or LYL1 protooncogene expression. CONCLUSION: Poor prognostic impact of TAL1 and/or LMO2 and/or LYL1 proto-oncogene expression indicate the need for extensive study on oncogenic rearrangement and immunophenotypic markers in T-ALL, and their relationship to treatment outcome. |
| first_indexed | 2024-04-10T14:22:11Z |
| format | Article |
| id | doaj.art-b81ce4f098fd45aea7c8caae301e35cf |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T14:22:11Z |
| publishDate | 2012-12-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-b81ce4f098fd45aea7c8caae301e35cf2023-02-15T16:09:16ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632012-12-0129432533310.5505/tjh.2012.13540TJH-13540Upregulation of T-Cell-Specific Transcription Factor Expression in Pediatric T-Cell Acute Lymphoblastic Leukemia (T-ALL)Müge Sayitoğlu0Yücel Erbilgin1Özden Hatırnaz2İnci Yıldız3Tiraje Celkan4Sema Anak5Ömer Devecioğlu6Gönül Aydoğan7Serap Karaman8Nazan Sarper9Çetin Timur10Ümit Üre11Uğur Özbek12İstanbul University, Department Of Genetics, Institute Of Experimental Medicine, İstanbul, Turkeyİstanbul University, Department Of Genetics, Institute Of Experimental Medicine, İstanbul, Turkeyİstanbul University, Department Of Genetics, Institute Of Experimental Medicine, İstanbul, Turkeyİstanbul University, Cerrahpaşa School Of Medicine, Department Of Pediatric Hematology, İstanbul, Turkeyİstanbul University, Cerrahpaşa School Of Medicine, Department Of Pediatric Hematology, İstanbul, Turkeyİstanbul University, İstanbul School Of Medicine, Department Of Pediatric Hematology, İstanbul, Turkeyİstanbul University, İstanbul School Of Medicine, Department Of Pediatric Hematology, İstanbul, TurkeyBakırköy Maternity And Children's Hospital, Department Of Pediatrics, İstanbul, TurkeyMinistry Of Health Şişli Etfal Teaching Hospital, Department Of Pediatric Hematology, İstanbul, TurkeyKocaeli School Of Medicine, Department Of Pediatric Hematology, Kocaeli, TurkeyMinistry Of Health Göztepe Teaching Hospital, Department Of Pediatric Hematology, İstanbul, TurkeyMinistry Of Health Bakırköy Sadi Konuk Teaching Hospital, Department Of Hematology, İstanbul, Turkeyİstanbul University, Department Of Genetics, Institute Of Experimental Medicine, İstanbul, TurkeyOBJECTIVE: T-cell acute lymphoblastic leukemia (T-ALL) is associated with recurrent chromosomal aberrations and abnormal ectopic gene expression during T-cell development. In order to gain insight into the pathogenesis of T-ALL this study aimed to measure the level of expression of 7 T-cell oncogenes (LMO2, LYL1, TAL1, TLX1, TLX3, BMI1, and CALM-AF10) in pediatric T-ALL patients. METHODS: LMO2, LYL1, TLX1, TLX3, BMI1, TAL1, and CALM-AF10 expression was measured using quantitative real-time PCR in 43 pediatric T-ALL patients. RESULTS: A high level of expression of LMO2, LYL1, TAL1, and BMI1 genes was observed in a large group of T-ALL. Several gene expression signatures indicative of leukemic arrest at specific stages of normal thymocyte development (LYL1 and LMO2) were highly expressed during the cortical and mature stages of T-cell development. Furthermore, upregulated TAL1 and BMI1 expression was observed in all phenotypic subgroups. In all, 6 of the patients had TLX1 and TLX3 proto-oncogene expression, which does not occur in normal cells, and none of the patients had CALM-AF10 fusion gene transcription. Expression of LYL1 alone and LMO2-LYL1 co-expression were associated with mediastinal involvement; however, high-level oncogene expression was not predictive of outcome in the present pediatric T-ALL patient group, but there was a trend towards a poor prognostic impact of TAL1 and/or LMO2 and/or LYL1 protooncogene expression. CONCLUSION: Poor prognostic impact of TAL1 and/or LMO2 and/or LYL1 proto-oncogene expression indicate the need for extensive study on oncogenic rearrangement and immunophenotypic markers in T-ALL, and their relationship to treatment outcome.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-13540t-allpediatrictranscription factorexpressionprognosis |
| spellingShingle | Müge Sayitoğlu Yücel Erbilgin Özden Hatırnaz İnci Yıldız Tiraje Celkan Sema Anak Ömer Devecioğlu Gönül Aydoğan Serap Karaman Nazan Sarper Çetin Timur Ümit Üre Uğur Özbek Upregulation of T-Cell-Specific Transcription Factor Expression in Pediatric T-Cell Acute Lymphoblastic Leukemia (T-ALL) Turkish Journal of Hematology t-all pediatric transcription factor expression prognosis |
| title | Upregulation of T-Cell-Specific Transcription Factor Expression in Pediatric T-Cell Acute Lymphoblastic Leukemia (T-ALL) |
| title_full | Upregulation of T-Cell-Specific Transcription Factor Expression in Pediatric T-Cell Acute Lymphoblastic Leukemia (T-ALL) |
| title_fullStr | Upregulation of T-Cell-Specific Transcription Factor Expression in Pediatric T-Cell Acute Lymphoblastic Leukemia (T-ALL) |
| title_full_unstemmed | Upregulation of T-Cell-Specific Transcription Factor Expression in Pediatric T-Cell Acute Lymphoblastic Leukemia (T-ALL) |
| title_short | Upregulation of T-Cell-Specific Transcription Factor Expression in Pediatric T-Cell Acute Lymphoblastic Leukemia (T-ALL) |
| title_sort | upregulation of t cell specific transcription factor expression in pediatric t cell acute lymphoblastic leukemia t all |
| topic | t-all pediatric transcription factor expression prognosis |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-13540 |
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