Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae)
Objective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa (T. spinosa) as well as to determine and quantify some of its polyphenols by high performance liquid chromatography with diode-array detection (HPLC-DAD). Methods:...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=1;spage=12;epage=17;aulast=dos |
| _version_ | 1818270510331461632 |
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| author | Francisca Sâmara Muniz dos Santos José Weverton Almeida Bezerra Jean Paul Kamdem Aline Augusti Boligon Marli Matiko Anraku Ana Raquel Pereira da Silva Kleber Ribeiro Fidelis Nadghia Figueiredo Leite Antônio Ivanildo Pinho Henrique Douglas Melo Coutinho José Edilson Gonçalves dos Santos |
| author_facet | Francisca Sâmara Muniz dos Santos José Weverton Almeida Bezerra Jean Paul Kamdem Aline Augusti Boligon Marli Matiko Anraku Ana Raquel Pereira da Silva Kleber Ribeiro Fidelis Nadghia Figueiredo Leite Antônio Ivanildo Pinho Henrique Douglas Melo Coutinho José Edilson Gonçalves dos Santos |
| author_sort | Francisca Sâmara Muniz dos Santos |
| collection | DOAJ |
| description | Objective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa (T. spinosa) as well as to determine and quantify some of its polyphenols by high performance liquid chromatography with diode-array detection (HPLC-DAD). Methods: The bacterial Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa strains, grown in Heart Agar Infusion, were tested. The drugs gentamicin, norfloxacin and imipenem were used to evaluate the modulating or antagonistic capacity of the T. spinosa extracts. The extract was analysed by HPLC-DAD to determine the main phenolic compounds. For the cell viability tests, individual heads of the Nauphoeta cinerea arthropod model were removed, homogenized in Trifluoromethyl ketone and centrifuged afterwards. Subsequently, 20 μL of NaNO2 were added to the biological material, except in the control group, to evaluate the protection capacity of the extracts. The homogenate of the insect heads was incubated for 2 h in tubes containing tetrazolium bromide. Results: HPLC-DAD demonstrated that the ethanolic extract of T. spinosa presented caffeic acid as the major compound. The ethanolic extract also showed neuroprotective effects at concentrations ≥ 10 μg/mL, while aqueous extract was shown to have a protective effect only at the concentration of 100 μg/mL. The aqueous extract demonstrated a clinically relevant antibacterial activity against the Staphylococcus aureus multidrug resistant strain - MDR, with MIC 512 μg/mL. However, when the extracts were associated with gentamicin and imipenem, a synergism was detected against Staphylococcus aureus and Escherichia coli MDR strains. Conclusions: Although it does not present an antibacterial action, the extracts of T. spinosa can be used in the pharmaceutical industries since its extracts show modulating action of drugs. Besides, these natural products have neuroprotective capacity. |
| first_indexed | 2024-12-12T21:11:25Z |
| format | Article |
| id | doaj.art-b81f3929eaf541fdb6883e5303274589 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-12T21:11:25Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-b81f3929eaf541fdb6883e53032745892022-12-22T00:11:52ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912019-01-0191121710.4103/2221-1691.250264Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae)Francisca Sâmara Muniz dos SantosJosé Weverton Almeida BezerraJean Paul KamdemAline Augusti BoligonMarli Matiko AnrakuAna Raquel Pereira da SilvaKleber Ribeiro FidelisNadghia Figueiredo LeiteAntônio Ivanildo PinhoHenrique Douglas Melo CoutinhoJosé Edilson Gonçalves dos SantosObjective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa (T. spinosa) as well as to determine and quantify some of its polyphenols by high performance liquid chromatography with diode-array detection (HPLC-DAD). Methods: The bacterial Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa strains, grown in Heart Agar Infusion, were tested. The drugs gentamicin, norfloxacin and imipenem were used to evaluate the modulating or antagonistic capacity of the T. spinosa extracts. The extract was analysed by HPLC-DAD to determine the main phenolic compounds. For the cell viability tests, individual heads of the Nauphoeta cinerea arthropod model were removed, homogenized in Trifluoromethyl ketone and centrifuged afterwards. Subsequently, 20 μL of NaNO2 were added to the biological material, except in the control group, to evaluate the protection capacity of the extracts. The homogenate of the insect heads was incubated for 2 h in tubes containing tetrazolium bromide. Results: HPLC-DAD demonstrated that the ethanolic extract of T. spinosa presented caffeic acid as the major compound. The ethanolic extract also showed neuroprotective effects at concentrations ≥ 10 μg/mL, while aqueous extract was shown to have a protective effect only at the concentration of 100 μg/mL. The aqueous extract demonstrated a clinically relevant antibacterial activity against the Staphylococcus aureus multidrug resistant strain - MDR, with MIC 512 μg/mL. However, when the extracts were associated with gentamicin and imipenem, a synergism was detected against Staphylococcus aureus and Escherichia coli MDR strains. Conclusions: Although it does not present an antibacterial action, the extracts of T. spinosa can be used in the pharmaceutical industries since its extracts show modulating action of drugs. Besides, these natural products have neuroprotective capacity.http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=1;spage=12;epage=17;aulast=dostarenaya spinosacleome spinosenauphoeta cinereasynergismantibiotic resistance |
| spellingShingle | Francisca Sâmara Muniz dos Santos José Weverton Almeida Bezerra Jean Paul Kamdem Aline Augusti Boligon Marli Matiko Anraku Ana Raquel Pereira da Silva Kleber Ribeiro Fidelis Nadghia Figueiredo Leite Antônio Ivanildo Pinho Henrique Douglas Melo Coutinho José Edilson Gonçalves dos Santos Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) Asian Pacific Journal of Tropical Biomedicine tarenaya spinosa cleome spinose nauphoeta cinerea synergism antibiotic resistance |
| title | Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
| title_full | Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
| title_fullStr | Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
| title_full_unstemmed | Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
| title_short | Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
| title_sort | polyphenolic composition antibacterial modulator and neuroprotective activity of tarenaya spinosa jacq raf cleomaceae |
| topic | tarenaya spinosa cleome spinose nauphoeta cinerea synergism antibiotic resistance |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=1;spage=12;epage=17;aulast=dos |
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