A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil
Wild poinsettia (<i>Euphorbia heterophylla</i> L.) is an important weed species in southern Brazil, especially due to the evolution of multiple herbicide resistance (e.g., acetolactate synthase (ALS)- inhibitors, protoporphyrinogen oxidase inhibitors, and glyphosate). The mechanism of re...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/8/1057 |
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| author | Rafael R. Mendes Hudson K. Takano Rubem S. Oliveira Fernando S. Adegas Todd A. Gaines Franck E. Dayan |
| author_facet | Rafael R. Mendes Hudson K. Takano Rubem S. Oliveira Fernando S. Adegas Todd A. Gaines Franck E. Dayan |
| author_sort | Rafael R. Mendes |
| collection | DOAJ |
| description | Wild poinsettia (<i>Euphorbia heterophylla</i> L.) is an important weed species in southern Brazil, especially due to the evolution of multiple herbicide resistance (e.g., acetolactate synthase (ALS)- inhibitors, protoporphyrinogen oxidase inhibitors, and glyphosate). The mechanism of resistance to imazamox was investigated in two wild poinsettia populations (R1 and R2) from southern Brazil and compared to a known susceptible (S) population. Imazamox dose-response experiments revealed high levels of resistance: 45-fold and 224.5-fold based on dry biomass reduction, for R1 and R2, respectively. Extremely high concentrations of imazamox (20,000 µM) were not sufficient to provide 50% inhibition of ALS enzyme activity (I<sub>50</sub>) for R1 or R2. Hence, resistance levels were estimated to be greater than 123-fold for both populations based on in vitro ALS assays. The <i>ALS</i> gene from all R1 and R2 plants had a Trp574Leu mutation. A genotyping assay was developed to discriminate resistant and susceptible alleles based on the Trp574Leu mutation. |
| first_indexed | 2024-03-10T18:19:12Z |
| format | Article |
| id | doaj.art-b8207cd063c940679093bdfc9afc5998 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T18:19:12Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-b8207cd063c940679093bdfc9afc59982023-11-20T07:31:09ZengMDPI AGAgronomy2073-43952020-07-01108105710.3390/agronomy10081057A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from BrazilRafael R. Mendes0Hudson K. Takano1Rubem S. Oliveira2Fernando S. Adegas3Todd A. Gaines4Franck E. Dayan5Agronomy Department, Maringá State University, 5790 Colombo Ave., Maringá, Paraná 87020-900, BrazilAgricultural Biology, Colorado State University, 307 University Ave., Fort Collins, CO 80523, USAAgronomy Department, Maringá State University, 5790 Colombo Ave., Maringá, Paraná 87020-900, BrazilEmbrapa Soybean, Jõao Carlos Strass Rd., Londrina, Paraná 86001-970, BrazilAgricultural Biology, Colorado State University, 307 University Ave., Fort Collins, CO 80523, USAAgricultural Biology, Colorado State University, 307 University Ave., Fort Collins, CO 80523, USAWild poinsettia (<i>Euphorbia heterophylla</i> L.) is an important weed species in southern Brazil, especially due to the evolution of multiple herbicide resistance (e.g., acetolactate synthase (ALS)- inhibitors, protoporphyrinogen oxidase inhibitors, and glyphosate). The mechanism of resistance to imazamox was investigated in two wild poinsettia populations (R1 and R2) from southern Brazil and compared to a known susceptible (S) population. Imazamox dose-response experiments revealed high levels of resistance: 45-fold and 224.5-fold based on dry biomass reduction, for R1 and R2, respectively. Extremely high concentrations of imazamox (20,000 µM) were not sufficient to provide 50% inhibition of ALS enzyme activity (I<sub>50</sub>) for R1 or R2. Hence, resistance levels were estimated to be greater than 123-fold for both populations based on in vitro ALS assays. The <i>ALS</i> gene from all R1 and R2 plants had a Trp574Leu mutation. A genotyping assay was developed to discriminate resistant and susceptible alleles based on the Trp574Leu mutation.https://www.mdpi.com/2073-4395/10/8/1057<i>Euphorbia heterophylla</i> L.acetolactate synthasetarget site mutationherbicide resistance mechanism |
| spellingShingle | Rafael R. Mendes Hudson K. Takano Rubem S. Oliveira Fernando S. Adegas Todd A. Gaines Franck E. Dayan A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil Agronomy <i>Euphorbia heterophylla</i> L. acetolactate synthase target site mutation herbicide resistance mechanism |
| title | A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil |
| title_full | A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil |
| title_fullStr | A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil |
| title_full_unstemmed | A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil |
| title_short | A Trp574Leu Target-Site Mutation Confers Imazamox Resistance in Multiple Herbicide-Resistant Wild Poinsettia Populations from Brazil |
| title_sort | trp574leu target site mutation confers imazamox resistance in multiple herbicide resistant wild poinsettia populations from brazil |
| topic | <i>Euphorbia heterophylla</i> L. acetolactate synthase target site mutation herbicide resistance mechanism |
| url | https://www.mdpi.com/2073-4395/10/8/1057 |
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