Bacterial Community Composition and Chromophoric Dissolved Organic Matter Differs with Culture Time of <i>Skeletonema dohrnii</i>
<i>Skeletonema dohrnii</i> is a common red tide microalgae occurring in the coastal waters and throughout the world. The associated heterotrophic or autotrophic bacteria play vital roles in regulating algal growth, production, and physiology. In this study, we investigated the detailed b...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Diversity |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-2818/13/4/150 |
| _version_ | 1797539496097480704 |
|---|---|
| author | Yang Liu Jinjun Kan Jing Yang Md Abu Noman Jun Sun |
| author_facet | Yang Liu Jinjun Kan Jing Yang Md Abu Noman Jun Sun |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | <i>Skeletonema dohrnii</i> is a common red tide microalgae occurring in the coastal waters and throughout the world. The associated heterotrophic or autotrophic bacteria play vital roles in regulating algal growth, production, and physiology. In this study, we investigated the detailed bacterial community structure associated with the growth of <i>S. dohrnii</i>’s using high-throughput sequencing-based on 16S rDNA. Our results demonstrated that Bacteroidetes (48.04%) and Proteobacteria (40.66%) in all samples accounted for the majority of bacterial populations. There was a significant linear regression relationship between the abundance of bacterial phyla and culture time. Notable shifts in bacterial community composition were observed during algal growth: Flavobacteriales accounted for the vast majority of sequences at the order level. Furthermore, the relative abundance of Rhodobacterales was gradually reduced during the whole growth process of <i>S. dohrnii</i> (0–12 days). However, beyond that, the relative abundance of <i>Marinobacter</i> was slowly increasing. It is noteworthy that five fluorophores (Peaks T<sub>1</sub>, T<sub>2</sub>, I, M, and A) were detected during the growth stage of <i>S. dohrnii</i>. The characteristic indexes (fluorescence index, humification index, and biological index) of chromophoric dissolved organic matter (CDOM) also varied with the culture time. In addition, the taxa of bacteria had certain effects on CDOM and they were inextricably linked to each other. |
| first_indexed | 2024-03-10T12:45:49Z |
| format | Article |
| id | doaj.art-b821a6fbcdac42f4b737dcb4ab637668 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T12:45:49Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-b821a6fbcdac42f4b737dcb4ab6376682023-11-21T13:30:02ZengMDPI AGDiversity1424-28182021-03-0113415010.3390/d13040150Bacterial Community Composition and Chromophoric Dissolved Organic Matter Differs with Culture Time of <i>Skeletonema dohrnii</i>Yang Liu0Jinjun Kan1Jing Yang2Md Abu Noman3Jun Sun4Institute of Marine Science and Technology, Shandong University, Qingdao 266237, ChinaStroud Water Research Center, 970 Spencer Road, Avondale, PA 19311, USASchool of Life Science, Shanxi University, Taiyuan 030006, ChinaCollege of Marine Science and Technology, China University of Geosciences (Wuhan), Wuhan 430074, ChinaCollege of Marine Science and Technology, China University of Geosciences (Wuhan), Wuhan 430074, China<i>Skeletonema dohrnii</i> is a common red tide microalgae occurring in the coastal waters and throughout the world. The associated heterotrophic or autotrophic bacteria play vital roles in regulating algal growth, production, and physiology. In this study, we investigated the detailed bacterial community structure associated with the growth of <i>S. dohrnii</i>’s using high-throughput sequencing-based on 16S rDNA. Our results demonstrated that Bacteroidetes (48.04%) and Proteobacteria (40.66%) in all samples accounted for the majority of bacterial populations. There was a significant linear regression relationship between the abundance of bacterial phyla and culture time. Notable shifts in bacterial community composition were observed during algal growth: Flavobacteriales accounted for the vast majority of sequences at the order level. Furthermore, the relative abundance of Rhodobacterales was gradually reduced during the whole growth process of <i>S. dohrnii</i> (0–12 days). However, beyond that, the relative abundance of <i>Marinobacter</i> was slowly increasing. It is noteworthy that five fluorophores (Peaks T<sub>1</sub>, T<sub>2</sub>, I, M, and A) were detected during the growth stage of <i>S. dohrnii</i>. The characteristic indexes (fluorescence index, humification index, and biological index) of chromophoric dissolved organic matter (CDOM) also varied with the culture time. In addition, the taxa of bacteria had certain effects on CDOM and they were inextricably linked to each other.https://www.mdpi.com/1424-2818/13/4/150microalgaebacteriahigh-throughput sequencingfluorophores |
| spellingShingle | Yang Liu Jinjun Kan Jing Yang Md Abu Noman Jun Sun Bacterial Community Composition and Chromophoric Dissolved Organic Matter Differs with Culture Time of <i>Skeletonema dohrnii</i> Diversity microalgae bacteria high-throughput sequencing fluorophores |
| title | Bacterial Community Composition and Chromophoric Dissolved Organic Matter Differs with Culture Time of <i>Skeletonema dohrnii</i> |
| title_full | Bacterial Community Composition and Chromophoric Dissolved Organic Matter Differs with Culture Time of <i>Skeletonema dohrnii</i> |
| title_fullStr | Bacterial Community Composition and Chromophoric Dissolved Organic Matter Differs with Culture Time of <i>Skeletonema dohrnii</i> |
| title_full_unstemmed | Bacterial Community Composition and Chromophoric Dissolved Organic Matter Differs with Culture Time of <i>Skeletonema dohrnii</i> |
| title_short | Bacterial Community Composition and Chromophoric Dissolved Organic Matter Differs with Culture Time of <i>Skeletonema dohrnii</i> |
| title_sort | bacterial community composition and chromophoric dissolved organic matter differs with culture time of i skeletonema dohrnii i |
| topic | microalgae bacteria high-throughput sequencing fluorophores |
| url | https://www.mdpi.com/1424-2818/13/4/150 |
| work_keys_str_mv | AT yangliu bacterialcommunitycompositionandchromophoricdissolvedorganicmatterdifferswithculturetimeofiskeletonemadohrniii AT jinjunkan bacterialcommunitycompositionandchromophoricdissolvedorganicmatterdifferswithculturetimeofiskeletonemadohrniii AT jingyang bacterialcommunitycompositionandchromophoricdissolvedorganicmatterdifferswithculturetimeofiskeletonemadohrniii AT mdabunoman bacterialcommunitycompositionandchromophoricdissolvedorganicmatterdifferswithculturetimeofiskeletonemadohrniii AT junsun bacterialcommunitycompositionandchromophoricdissolvedorganicmatterdifferswithculturetimeofiskeletonemadohrniii |