The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients
The rotavirus vaccine is a live vaccine, and there is a possibility of infection by the virus strain used in the vaccine. We investigated the process of determining whether an infection was caused by the vaccine strain in a severe complex immunodeficiency (SCID) patient with rotavirus infection. The...
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| Format: | Article |
| Language: | English |
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Korea Disease Control and Prevention Agency
2021-08-01
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| Series: | Osong Public Health and Research Perspectives |
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| Online Access: | http://ophrp.org/upload/pdf/j-phrp-2021-0079.pdf |
| _version_ | 1797726740032782336 |
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| author | Su-Jin Chae Seung-Rye Cho Wooyoung Choi Myung-Guk Han Deog-Yong Lee |
| author_facet | Su-Jin Chae Seung-Rye Cho Wooyoung Choi Myung-Guk Han Deog-Yong Lee |
| author_sort | Su-Jin Chae |
| collection | DOAJ |
| description | The rotavirus vaccine is a live vaccine, and there is a possibility of infection by the virus strain used in the vaccine. We investigated the process of determining whether an infection was caused by the vaccine strain in a severe complex immunodeficiency (SCID) patient with rotavirus infection. The patient was vaccinated with RotaTeq prior to being diagnosed with SCID. The testing process was conducted in the following order: confirming rotavirus infection, determining its genotype, and confirming the vaccine strain. Rotavirus infection was confirmed through enzyme immunoassay and VP6 gene detection. G1 and P[8] were identified by multiplex polymerase chain reaction for the genotype, and G3 was further identified using a single primer. By detecting the fingerprint gene (WC3) of RotaTeq, it was confirmed that the detected virus was the vaccine strain. Genotypes G1 and P[8] were identified, and the infection was suspected of having been caused by rotavirus G1P[8]. G1P[8] is the most commonly detected genotype worldwide and is not included in the recombinant strains used in vaccines. Therefore, the infection was confirmed to have been caused by the vaccine strain by analyzing the genetic relationship between VP4 and VP7. Rotavirus infection by the vaccine strain can be identified through genotyping and fingerprint gene detection. However, genetic linkage analysis will also help to identify vaccine strains. |
| first_indexed | 2024-03-12T10:49:50Z |
| format | Article |
| id | doaj.art-b8223bfb2e024d58b2baa630e58b76fb |
| institution | Directory Open Access Journal |
| issn | 2210-9099 2210-9110 |
| language | English |
| last_indexed | 2024-03-12T10:49:50Z |
| publishDate | 2021-08-01 |
| publisher | Korea Disease Control and Prevention Agency |
| record_format | Article |
| series | Osong Public Health and Research Perspectives |
| spelling | doaj.art-b8223bfb2e024d58b2baa630e58b76fb2023-09-02T07:10:29ZengKorea Disease Control and Prevention AgencyOsong Public Health and Research Perspectives2210-90992210-91102021-08-0112426927310.24171/j.phrp.2021.0079624The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patientsSu-Jin Chae0Seung-Rye Cho1Wooyoung Choi2Myung-Guk Han3Deog-Yong Lee4 Division of Viral Diseases, Bureau of Infectious Diseases Diagnosis Control, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of Viral Diseases, Bureau of Infectious Diseases Diagnosis Control, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of Viral Diseases, Bureau of Infectious Diseases Diagnosis Control, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of Viral Diseases, Bureau of Infectious Diseases Diagnosis Control, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of Viral Diseases, Bureau of Infectious Diseases Diagnosis Control, Korea Disease Control and Prevention Agency, Cheongju, KoreaThe rotavirus vaccine is a live vaccine, and there is a possibility of infection by the virus strain used in the vaccine. We investigated the process of determining whether an infection was caused by the vaccine strain in a severe complex immunodeficiency (SCID) patient with rotavirus infection. The patient was vaccinated with RotaTeq prior to being diagnosed with SCID. The testing process was conducted in the following order: confirming rotavirus infection, determining its genotype, and confirming the vaccine strain. Rotavirus infection was confirmed through enzyme immunoassay and VP6 gene detection. G1 and P[8] were identified by multiplex polymerase chain reaction for the genotype, and G3 was further identified using a single primer. By detecting the fingerprint gene (WC3) of RotaTeq, it was confirmed that the detected virus was the vaccine strain. Genotypes G1 and P[8] were identified, and the infection was suspected of having been caused by rotavirus G1P[8]. G1P[8] is the most commonly detected genotype worldwide and is not included in the recombinant strains used in vaccines. Therefore, the infection was confirmed to have been caused by the vaccine strain by analyzing the genetic relationship between VP4 and VP7. Rotavirus infection by the vaccine strain can be identified through genotyping and fingerprint gene detection. However, genetic linkage analysis will also help to identify vaccine strains.http://ophrp.org/upload/pdf/j-phrp-2021-0079.pdfinfantile diarrhealaboratory testrotavirus infectionsrotavirus vaccinessevere combined immunodeficiency |
| spellingShingle | Su-Jin Chae Seung-Rye Cho Wooyoung Choi Myung-Guk Han Deog-Yong Lee The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients Osong Public Health and Research Perspectives infantile diarrhea laboratory test rotavirus infections rotavirus vaccines severe combined immunodeficiency |
| title | The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients |
| title_full | The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients |
| title_fullStr | The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients |
| title_full_unstemmed | The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients |
| title_short | The laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients |
| title_sort | laboratory test procedure to confirm rotavirus vaccine infection in severe complex immunodeficiency patients |
| topic | infantile diarrhea laboratory test rotavirus infections rotavirus vaccines severe combined immunodeficiency |
| url | http://ophrp.org/upload/pdf/j-phrp-2021-0079.pdf |
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