10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies
Objectives: Cumulus cells play a crucial role as essential mediators in the maturation of ova. Ginger contains 10-gingerol, which induces apoptosis in colon cancer cells. Based on this hypothesis, this study aimed to determine whether 10-gingerol is able to induce apoptosis in normal cells, namely,...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-10-01
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| Series: | Journal of Taibah University Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S165836121730094X |
| _version_ | 1828531797861859328 |
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| author | Kiptiyah Kiptiyah, D.Sc Widodo Widodo, Ph.D Gatot Ciptadi, D.Sc Aulanni'am Aulanni'am, DVM Mohammad A. Widodo, Ph.D Sutiman B. Sumitro, D.Sc |
| author_facet | Kiptiyah Kiptiyah, D.Sc Widodo Widodo, Ph.D Gatot Ciptadi, D.Sc Aulanni'am Aulanni'am, DVM Mohammad A. Widodo, Ph.D Sutiman B. Sumitro, D.Sc |
| author_sort | Kiptiyah Kiptiyah, D.Sc |
| collection | DOAJ |
| description | Objectives: Cumulus cells play a crucial role as essential mediators in the maturation of ova. Ginger contains 10-gingerol, which induces apoptosis in colon cancer cells. Based on this hypothesis, this study aimed to determine whether 10-gingerol is able to induce apoptosis in normal cells, namely, cumulus cells.
Methods: This study used an in vitro analysis by culturing Cumulus cells in M199 containing 10-gingerol in various concentrations (12, 16, and 20 μM) and later detected early apoptotic activity using an Annexin V-FITC detection kit.
Result: The in vitro data revealed that the number of apoptosis cells increased along with the period of incubation as follows: 12 μM (63.71% ± 2.192%); 16 μM (74.51% ± 4.596%); and 20 μM (78.795% ± 1.435%). The substance 10-gingerol induces apoptosis in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT-1.
Conclusions: These findings indicate that further examination is warranted for 10-gingerol as a contraception agent. |
| first_indexed | 2024-12-11T22:43:03Z |
| format | Article |
| id | doaj.art-b824260d8d454e239eadff6e059c3814 |
| institution | Directory Open Access Journal |
| issn | 1658-3612 |
| language | English |
| last_indexed | 2024-12-11T22:43:03Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Taibah University Medical Sciences |
| spelling | doaj.art-b824260d8d454e239eadff6e059c38142022-12-22T00:47:43ZengElsevierJournal of Taibah University Medical Sciences1658-36122017-10-0112539740610.1016/j.jtumed.2017.05.01210-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studiesKiptiyah Kiptiyah, D.Sc0Widodo Widodo, Ph.D1Gatot Ciptadi, D.Sc2Aulanni'am Aulanni'am, DVM3Mohammad A. Widodo, Ph.D4Sutiman B. Sumitro, D.Sc5Department of Biology, Faculty of Sciences and Technology, Maulana Malik Ibrahim Islamic State University of Malang, IndonesiaDepartment of Biology, Faculty of Sciences, Brawijaya University, Malang, IndonesiaDepartment of Reproduction, Faculty of Husbandry, Brawijaya University, Malang, IndonesiaDepartment of Chemistry, Faculty of Sciences, Brawijaya University, Malang, IndonesiaDepartment of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, IndonesiaDepartment of Biology, Faculty of Sciences, Brawijaya University, Malang, IndonesiaObjectives: Cumulus cells play a crucial role as essential mediators in the maturation of ova. Ginger contains 10-gingerol, which induces apoptosis in colon cancer cells. Based on this hypothesis, this study aimed to determine whether 10-gingerol is able to induce apoptosis in normal cells, namely, cumulus cells. Methods: This study used an in vitro analysis by culturing Cumulus cells in M199 containing 10-gingerol in various concentrations (12, 16, and 20 μM) and later detected early apoptotic activity using an Annexin V-FITC detection kit. Result: The in vitro data revealed that the number of apoptosis cells increased along with the period of incubation as follows: 12 μM (63.71% ± 2.192%); 16 μM (74.51% ± 4.596%); and 20 μM (78.795% ± 1.435%). The substance 10-gingerol induces apoptosis in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT-1. Conclusions: These findings indicate that further examination is warranted for 10-gingerol as a contraception agent.http://www.sciencedirect.com/science/article/pii/S165836121730094X10-GingerolApoptosisCumulus cellsHTR1AIn silicoIn vitro |
| spellingShingle | Kiptiyah Kiptiyah, D.Sc Widodo Widodo, Ph.D Gatot Ciptadi, D.Sc Aulanni'am Aulanni'am, DVM Mohammad A. Widodo, Ph.D Sutiman B. Sumitro, D.Sc 10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies Journal of Taibah University Medical Sciences 10-Gingerol Apoptosis Cumulus cells HTR1A In silico In vitro |
| title | 10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies |
| title_full | 10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies |
| title_fullStr | 10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies |
| title_full_unstemmed | 10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies |
| title_short | 10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies |
| title_sort | 10 gingerol as an inducer of apoptosis through htr1a in cumulus cells in vitro and in silico studies |
| topic | 10-Gingerol Apoptosis Cumulus cells HTR1A In silico In vitro |
| url | http://www.sciencedirect.com/science/article/pii/S165836121730094X |
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