Enhancement of Glucose Uptake in 3T3-L1 Adipocytes by Toona sinensis Leaf Extract
The effects of substances extracted from Toona sinensis leaves, using 50% alcohol/water, on cellular [3H]-2-deoxyglucose uptake in differentiated cultured 3T3-L1 adipocytes were investigated. Following treatment of cells with 0.001, 0.01, or 0.1 mg/mL extracts for 60 minutes, [3H]-2-deoxyglucose upt...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley
2003-07-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X09704334 |
| _version_ | 1818274725916311552 |
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| author | Yu-Chiao Yang Jia-Huae Hwang Show-Jen Hong |
| author_facet | Yu-Chiao Yang Jia-Huae Hwang Show-Jen Hong |
| author_sort | Yu-Chiao Yang |
| collection | DOAJ |
| description | The effects of substances extracted from Toona sinensis leaves, using 50% alcohol/water, on cellular [3H]-2-deoxyglucose uptake in differentiated cultured 3T3-L1 adipocytes were investigated. Following treatment of cells with 0.001, 0.01, or 0.1 mg/mL extracts for 60 minutes, [3H]-2-deoxyglucose uptake increased from a basal value of 0.23 nmol/min/mg protein to 0.30, 0.33, and 0.38 nmol/min/mg protein, respectively. In insulin-stimulated cells, cellular [3H]-2-deoxyglucose uptake was enhanced by Toona sinensis leaf extract from a basal value of 0.35 nmol/min/mg protein to 0.41, 0.46, and 0.52 nmol/min/mg protein, respectively. Cellular glucose uptake was also enhanced by Toona sinensis leaf extract after incubation of cells with 20 mM glucose for 48 hours. Cellular glucose uptake with a combination of Toona sinensis leaf extract and insulin was significantly inhibited by pretreatment of cells with the protein synthesis inhibitor cycloheximide and the protein kinase C inhibitor calphostin C in normal-, medium- and high-glucose media. However, the glucose uptake-enhancing effect of Toona sinensis leaf extract was not diminished by cycloheximide and calphostin C in the absence of insulin. These results indicate that enhancement of cellular glucose uptake by Toona sinensis leaf extract in basal and insulin-stimulated 3T3-L1 adipocytes may be mediated by distinct mechanisms. |
| first_indexed | 2024-12-12T22:18:26Z |
| format | Article |
| id | doaj.art-b8277b682e2a4ed68da115509443745b |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-12-12T22:18:26Z |
| publishDate | 2003-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-b8277b682e2a4ed68da115509443745b2022-12-22T00:10:00ZengWileyKaohsiung Journal of Medical Sciences1607-551X2003-07-0119732733210.1016/S1607-551X(09)70433-4Enhancement of Glucose Uptake in 3T3-L1 Adipocytes by Toona sinensis Leaf ExtractYu-Chiao YangJia-Huae HwangShow-Jen HongThe effects of substances extracted from Toona sinensis leaves, using 50% alcohol/water, on cellular [3H]-2-deoxyglucose uptake in differentiated cultured 3T3-L1 adipocytes were investigated. Following treatment of cells with 0.001, 0.01, or 0.1 mg/mL extracts for 60 minutes, [3H]-2-deoxyglucose uptake increased from a basal value of 0.23 nmol/min/mg protein to 0.30, 0.33, and 0.38 nmol/min/mg protein, respectively. In insulin-stimulated cells, cellular [3H]-2-deoxyglucose uptake was enhanced by Toona sinensis leaf extract from a basal value of 0.35 nmol/min/mg protein to 0.41, 0.46, and 0.52 nmol/min/mg protein, respectively. Cellular glucose uptake was also enhanced by Toona sinensis leaf extract after incubation of cells with 20 mM glucose for 48 hours. Cellular glucose uptake with a combination of Toona sinensis leaf extract and insulin was significantly inhibited by pretreatment of cells with the protein synthesis inhibitor cycloheximide and the protein kinase C inhibitor calphostin C in normal-, medium- and high-glucose media. However, the glucose uptake-enhancing effect of Toona sinensis leaf extract was not diminished by cycloheximide and calphostin C in the absence of insulin. These results indicate that enhancement of cellular glucose uptake by Toona sinensis leaf extract in basal and insulin-stimulated 3T3-L1 adipocytes may be mediated by distinct mechanisms.http://www.sciencedirect.com/science/article/pii/S1607551X097043342-deoxyglucose uptake3T3-L1 adipocytesToona sinensis leaf |
| spellingShingle | Yu-Chiao Yang Jia-Huae Hwang Show-Jen Hong Enhancement of Glucose Uptake in 3T3-L1 Adipocytes by Toona sinensis Leaf Extract Kaohsiung Journal of Medical Sciences 2-deoxyglucose uptake 3T3-L1 adipocytes Toona sinensis leaf |
| title | Enhancement of Glucose Uptake in 3T3-L1 Adipocytes by Toona sinensis Leaf Extract |
| title_full | Enhancement of Glucose Uptake in 3T3-L1 Adipocytes by Toona sinensis Leaf Extract |
| title_fullStr | Enhancement of Glucose Uptake in 3T3-L1 Adipocytes by Toona sinensis Leaf Extract |
| title_full_unstemmed | Enhancement of Glucose Uptake in 3T3-L1 Adipocytes by Toona sinensis Leaf Extract |
| title_short | Enhancement of Glucose Uptake in 3T3-L1 Adipocytes by Toona sinensis Leaf Extract |
| title_sort | enhancement of glucose uptake in 3t3 l1 adipocytes by toona sinensis leaf extract |
| topic | 2-deoxyglucose uptake 3T3-L1 adipocytes Toona sinensis leaf |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X09704334 |
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