Organization of Circadian Behavior Relies on Glycinergic Transmission
The small ventral lateral neurons (sLNvs) constitute a central circadian pacemaker in the Drosophila brain. They organize daily locomotor activity, partly through the release of the neuropeptide pigment-dispersing factor (PDF), coordinating the action of the remaining clusters required for network s...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-04-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717303662 |
| _version_ | 1811213431314841600 |
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| author | Lia Frenkel Nara I. Muraro Andrea N. Beltrán González María S. Marcora Guillermo Bernabó Christiane Hermann-Luibl Juan I. Romero Charlotte Helfrich-Förster Eduardo M. Castaño Cristina Marino-Busjle Daniel J. Calvo M. Fernanda Ceriani |
| author_facet | Lia Frenkel Nara I. Muraro Andrea N. Beltrán González María S. Marcora Guillermo Bernabó Christiane Hermann-Luibl Juan I. Romero Charlotte Helfrich-Förster Eduardo M. Castaño Cristina Marino-Busjle Daniel J. Calvo M. Fernanda Ceriani |
| author_sort | Lia Frenkel |
| collection | DOAJ |
| description | The small ventral lateral neurons (sLNvs) constitute a central circadian pacemaker in the Drosophila brain. They organize daily locomotor activity, partly through the release of the neuropeptide pigment-dispersing factor (PDF), coordinating the action of the remaining clusters required for network synchronization. Despite extensive efforts, the basic principles underlying communication among circadian clusters remain obscure. We identified classical neurotransmitters released by sLNvs through disruption of specific transporters. Adult-specific RNAi-mediated downregulation of the glycine transporter or impairment of glycine synthesis in LNv neurons increased period length by nearly an hour without affecting rhythmicity of locomotor activity. Electrophysiological recordings showed that glycine reduces spiking frequency in circadian neurons. Interestingly, downregulation of glycine receptor subunits in specific sLNv targets impaired rhythmicity, revealing involvement of glycine in information processing within the network. These data identify glycinergic inhibition of specific targets as a cue that contributes to the synchronization of the circadian network. |
| first_indexed | 2024-04-12T05:46:45Z |
| format | Article |
| id | doaj.art-b828befe10e04b1ba42b3b1afe8e0517 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-12T05:46:45Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-b828befe10e04b1ba42b3b1afe8e05172022-12-22T03:45:26ZengElsevierCell Reports2211-12472017-04-01191728510.1016/j.celrep.2017.03.034Organization of Circadian Behavior Relies on Glycinergic TransmissionLia Frenkel0Nara I. Muraro1Andrea N. Beltrán González2María S. Marcora3Guillermo Bernabó4Christiane Hermann-Luibl5Juan I. Romero6Charlotte Helfrich-Förster7Eduardo M. Castaño8Cristina Marino-Busjle9Daniel J. Calvo10M. Fernanda Ceriani11Laboratorio de Genética del Comportamiento, Fundación Instituto Leloir (FIL) IIBBA-CONICET, 1405BWE Buenos Aires, ArgentinaLaboratorio de Genética del Comportamiento, Fundación Instituto Leloir (FIL) IIBBA-CONICET, 1405BWE Buenos Aires, ArgentinaLaboratorio de Neurobiología Celular y Molecular, INGEBI-CONICET, C1428ADN Buenos Aires, ArgentinaLaboratorio de Amiloidosis y Neurodegeneración, FIL-IIBBA-CONICET, 1405BWE Buenos Aires, ArgentinaLaboratorio de Genética del Comportamiento, Fundación Instituto Leloir (FIL) IIBBA-CONICET, 1405BWE Buenos Aires, ArgentinaDepartment of Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Wuerzburg, Wuerzburg 97070, GermanyLaboratorio de Genética del Comportamiento, Fundación Instituto Leloir (FIL) IIBBA-CONICET, 1405BWE Buenos Aires, ArgentinaDepartment of Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Wuerzburg, Wuerzburg 97070, GermanyLaboratorio de Amiloidosis y Neurodegeneración, FIL-IIBBA-CONICET, 1405BWE Buenos Aires, ArgentinaLaboratorio de Bioinformática Estructural, FIL-IIBBA-CONICET, 1405BWE Buenos Aires, ArgentinaLaboratorio de Neurobiología Celular y Molecular, INGEBI-CONICET, C1428ADN Buenos Aires, ArgentinaLaboratorio de Genética del Comportamiento, Fundación Instituto Leloir (FIL) IIBBA-CONICET, 1405BWE Buenos Aires, ArgentinaThe small ventral lateral neurons (sLNvs) constitute a central circadian pacemaker in the Drosophila brain. They organize daily locomotor activity, partly through the release of the neuropeptide pigment-dispersing factor (PDF), coordinating the action of the remaining clusters required for network synchronization. Despite extensive efforts, the basic principles underlying communication among circadian clusters remain obscure. We identified classical neurotransmitters released by sLNvs through disruption of specific transporters. Adult-specific RNAi-mediated downregulation of the glycine transporter or impairment of glycine synthesis in LNv neurons increased period length by nearly an hour without affecting rhythmicity of locomotor activity. Electrophysiological recordings showed that glycine reduces spiking frequency in circadian neurons. Interestingly, downregulation of glycine receptor subunits in specific sLNv targets impaired rhythmicity, revealing involvement of glycine in information processing within the network. These data identify glycinergic inhibition of specific targets as a cue that contributes to the synchronization of the circadian network.http://www.sciencedirect.com/science/article/pii/S2211124717303662Drosophilacircadian rhythmssleep-wake cyclesglycineinhibitory neurotransmissionglycine transporterglycine receptor subunit |
| spellingShingle | Lia Frenkel Nara I. Muraro Andrea N. Beltrán González María S. Marcora Guillermo Bernabó Christiane Hermann-Luibl Juan I. Romero Charlotte Helfrich-Förster Eduardo M. Castaño Cristina Marino-Busjle Daniel J. Calvo M. Fernanda Ceriani Organization of Circadian Behavior Relies on Glycinergic Transmission Cell Reports Drosophila circadian rhythms sleep-wake cycles glycine inhibitory neurotransmission glycine transporter glycine receptor subunit |
| title | Organization of Circadian Behavior Relies on Glycinergic Transmission |
| title_full | Organization of Circadian Behavior Relies on Glycinergic Transmission |
| title_fullStr | Organization of Circadian Behavior Relies on Glycinergic Transmission |
| title_full_unstemmed | Organization of Circadian Behavior Relies on Glycinergic Transmission |
| title_short | Organization of Circadian Behavior Relies on Glycinergic Transmission |
| title_sort | organization of circadian behavior relies on glycinergic transmission |
| topic | Drosophila circadian rhythms sleep-wake cycles glycine inhibitory neurotransmission glycine transporter glycine receptor subunit |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717303662 |
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