Characterization of digestive enzymes of bruchid parasitoids-initial steps for environmental risk assessment of genetically modified legumes.
Genetically modified (GM) legumes expressing the α-amylase inhibitor 1 (αAI-1) from Phaseolus vulgaris L. or cysteine protease inhibitors are resistant to several bruchid pests (Coleoptera: Chrysomelidae). In addition, the combination of plant resistance factors together with hymenopteran parasitoid...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3353970?pdf=render |
| _version_ | 1818257160112439296 |
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| author | Fernando Álvarez-Alfageme Christoph Lüthi Jörg Romeis |
| author_facet | Fernando Álvarez-Alfageme Christoph Lüthi Jörg Romeis |
| author_sort | Fernando Álvarez-Alfageme |
| collection | DOAJ |
| description | Genetically modified (GM) legumes expressing the α-amylase inhibitor 1 (αAI-1) from Phaseolus vulgaris L. or cysteine protease inhibitors are resistant to several bruchid pests (Coleoptera: Chrysomelidae). In addition, the combination of plant resistance factors together with hymenopteran parasitoids can substantially increase the bruchid control provided by the resistance alone. If the strategy of combining a bruchid-resistant GM legume and biological control is to be effective, the insecticidal trait must not adversely affect bruchid antagonists. The environmental risk assessment of such GM legumes includes the characterization of the targeted enzymes in the beneficial species and the assessment of the in vitro susceptibility to the resistance factor. The digestive physiology of bruchid parasitoids remain relatively unknown, and their susceptibility to αAI-1 has never been investigated. We have detected α-amylase and serine protease activities in all five bruchid parasitoid species tested. Thus, the deployment of GM legumes expressing cysteine protease inhibitors to control bruchids should be compatible with the use of parasitoids. In vitro inhibition studies showed that sensitivity of α-amylase activity to αAI-1 in the parasitoids was comparable to that in the target species. Direct feeding assays revealed that harmful effects of α-amylase inhibitors on bruchid parasitoids cannot be discounted and need further evaluation. |
| first_indexed | 2024-12-12T17:39:14Z |
| format | Article |
| id | doaj.art-b82a6f6290754684ac087bc95941bea2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T17:39:14Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b82a6f6290754684ac087bc95941bea22022-12-22T00:17:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3686210.1371/journal.pone.0036862Characterization of digestive enzymes of bruchid parasitoids-initial steps for environmental risk assessment of genetically modified legumes.Fernando Álvarez-AlfagemeChristoph LüthiJörg RomeisGenetically modified (GM) legumes expressing the α-amylase inhibitor 1 (αAI-1) from Phaseolus vulgaris L. or cysteine protease inhibitors are resistant to several bruchid pests (Coleoptera: Chrysomelidae). In addition, the combination of plant resistance factors together with hymenopteran parasitoids can substantially increase the bruchid control provided by the resistance alone. If the strategy of combining a bruchid-resistant GM legume and biological control is to be effective, the insecticidal trait must not adversely affect bruchid antagonists. The environmental risk assessment of such GM legumes includes the characterization of the targeted enzymes in the beneficial species and the assessment of the in vitro susceptibility to the resistance factor. The digestive physiology of bruchid parasitoids remain relatively unknown, and their susceptibility to αAI-1 has never been investigated. We have detected α-amylase and serine protease activities in all five bruchid parasitoid species tested. Thus, the deployment of GM legumes expressing cysteine protease inhibitors to control bruchids should be compatible with the use of parasitoids. In vitro inhibition studies showed that sensitivity of α-amylase activity to αAI-1 in the parasitoids was comparable to that in the target species. Direct feeding assays revealed that harmful effects of α-amylase inhibitors on bruchid parasitoids cannot be discounted and need further evaluation.http://europepmc.org/articles/PMC3353970?pdf=render |
| spellingShingle | Fernando Álvarez-Alfageme Christoph Lüthi Jörg Romeis Characterization of digestive enzymes of bruchid parasitoids-initial steps for environmental risk assessment of genetically modified legumes. PLoS ONE |
| title | Characterization of digestive enzymes of bruchid parasitoids-initial steps for environmental risk assessment of genetically modified legumes. |
| title_full | Characterization of digestive enzymes of bruchid parasitoids-initial steps for environmental risk assessment of genetically modified legumes. |
| title_fullStr | Characterization of digestive enzymes of bruchid parasitoids-initial steps for environmental risk assessment of genetically modified legumes. |
| title_full_unstemmed | Characterization of digestive enzymes of bruchid parasitoids-initial steps for environmental risk assessment of genetically modified legumes. |
| title_short | Characterization of digestive enzymes of bruchid parasitoids-initial steps for environmental risk assessment of genetically modified legumes. |
| title_sort | characterization of digestive enzymes of bruchid parasitoids initial steps for environmental risk assessment of genetically modified legumes |
| url | http://europepmc.org/articles/PMC3353970?pdf=render |
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