EphA7 promotes myogenic differentiation via cell-cell contact
The conversion of proliferating skeletal muscle precursors (myoblasts) to terminally-differentiated myocytes is a critical step in skeletal muscle development and repair. We show that EphA7, a juxtacrine signaling receptor, is expressed on myocytes during embryonic and fetal myogenesis and on nascen...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2020-04-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/53689 |
| _version_ | 1811199635079823360 |
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| author | Laura L Arnold Alessandra Cecchini Danny A Stark Jacqueline Ihnat Rebecca N Craigg Amory Carter Sammy Zino DDW Cornelison |
| author_facet | Laura L Arnold Alessandra Cecchini Danny A Stark Jacqueline Ihnat Rebecca N Craigg Amory Carter Sammy Zino DDW Cornelison |
| author_sort | Laura L Arnold |
| collection | DOAJ |
| description | The conversion of proliferating skeletal muscle precursors (myoblasts) to terminally-differentiated myocytes is a critical step in skeletal muscle development and repair. We show that EphA7, a juxtacrine signaling receptor, is expressed on myocytes during embryonic and fetal myogenesis and on nascent myofibers during muscle regeneration in vivo. In EphA7-/- mice, hindlimb muscles possess fewer myofibers at birth, and those myofibers are reduced in size and have fewer myonuclei and reduced overall numbers of precursor cells throughout postnatal life. Adult EphA7-/- mice have reduced numbers of satellite cells and exhibit delayed and protracted muscle regeneration, and satellite cell-derived myogenic cells from EphA7-/- mice are delayed in their expression of differentiation markers in vitro. Exogenous EphA7 extracellular domain will rescue the null phenotype in vitro, and will also enhance commitment to differentiation in WT cells. We propose a model in which EphA7 expression on differentiated myocytes promotes commitment of adjacent myoblasts to terminal differentiation. |
| first_indexed | 2024-04-12T01:50:45Z |
| format | Article |
| id | doaj.art-b82ab7765f2e477890246f062a19934f |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:50:45Z |
| publishDate | 2020-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-b82ab7765f2e477890246f062a19934f2022-12-22T03:52:56ZengeLife Sciences Publications LtdeLife2050-084X2020-04-01910.7554/eLife.53689EphA7 promotes myogenic differentiation via cell-cell contactLaura L Arnold0Alessandra Cecchini1Danny A Stark2Jacqueline Ihnat3Rebecca N Craigg4Amory Carter5Sammy Zino6DDW Cornelison7https://orcid.org/0000-0003-4287-7524Division of Biological Sciences, University of Missouri, Columbia, United StatesDivision of Biological Sciences, University of Missouri, Columbia, United States; Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, United StatesDivision of Biological Sciences, University of Missouri, Columbia, United StatesDivision of Biological Sciences, University of Missouri, Columbia, United StatesDivision of Biological Sciences, University of Missouri, Columbia, United StatesDivision of Biological Sciences, University of Missouri, Columbia, United StatesDivision of Biological Sciences, University of Missouri, Columbia, United StatesDivision of Biological Sciences, University of Missouri, Columbia, United States; Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, United StatesThe conversion of proliferating skeletal muscle precursors (myoblasts) to terminally-differentiated myocytes is a critical step in skeletal muscle development and repair. We show that EphA7, a juxtacrine signaling receptor, is expressed on myocytes during embryonic and fetal myogenesis and on nascent myofibers during muscle regeneration in vivo. In EphA7-/- mice, hindlimb muscles possess fewer myofibers at birth, and those myofibers are reduced in size and have fewer myonuclei and reduced overall numbers of precursor cells throughout postnatal life. Adult EphA7-/- mice have reduced numbers of satellite cells and exhibit delayed and protracted muscle regeneration, and satellite cell-derived myogenic cells from EphA7-/- mice are delayed in their expression of differentiation markers in vitro. Exogenous EphA7 extracellular domain will rescue the null phenotype in vitro, and will also enhance commitment to differentiation in WT cells. We propose a model in which EphA7 expression on differentiated myocytes promotes commitment of adjacent myoblasts to terminal differentiation.https://elifesciences.org/articles/53689myogenesisEph/ephrincommunity effect |
| spellingShingle | Laura L Arnold Alessandra Cecchini Danny A Stark Jacqueline Ihnat Rebecca N Craigg Amory Carter Sammy Zino DDW Cornelison EphA7 promotes myogenic differentiation via cell-cell contact eLife myogenesis Eph/ephrin community effect |
| title | EphA7 promotes myogenic differentiation via cell-cell contact |
| title_full | EphA7 promotes myogenic differentiation via cell-cell contact |
| title_fullStr | EphA7 promotes myogenic differentiation via cell-cell contact |
| title_full_unstemmed | EphA7 promotes myogenic differentiation via cell-cell contact |
| title_short | EphA7 promotes myogenic differentiation via cell-cell contact |
| title_sort | epha7 promotes myogenic differentiation via cell cell contact |
| topic | myogenesis Eph/ephrin community effect |
| url | https://elifesciences.org/articles/53689 |
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