Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a three-year period
Mannheimia haemolytica isolated from feedlot cattle were tested for tulathromycin resistance. Cattle were sampled over a three-year period, starting 12 months after approval of tulathromycin for prevention and treatment of bovine respiratory disease. Nasopharyngeal samples from approximately 5,814...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2013-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00297/full |
| _version_ | 1811229552691642368 |
|---|---|
| author | Trevor W. Alexander Shaun R. Cook Cassidy L. Klima Ed eTopp Tim A. McAllister |
| author_facet | Trevor W. Alexander Shaun R. Cook Cassidy L. Klima Ed eTopp Tim A. McAllister |
| author_sort | Trevor W. Alexander |
| collection | DOAJ |
| description | Mannheimia haemolytica isolated from feedlot cattle were tested for tulathromycin resistance. Cattle were sampled over a three-year period, starting 12 months after approval of tulathromycin for prevention and treatment of bovine respiratory disease. Nasopharyngeal samples from approximately 5,814 cattle were collected when cattle entered feedlots (N = 4) and again from the same cattle after ≥ 60 d on feed. The antimicrobial use history for each animal was recorded. Mannheimia haemolytica was isolated from 796 (13.7%) entry samples and 1,038 (20.6%) ≥ 60 d samples. Of the cattle positive for M. haemolytica, 18.5%, 2.9%, and 2.4% were administered therapeutic concentrations of tulathromycin, tilmicosin, or tylosin tartrate, respectively. In addition, 13.2% were administered subtherapeutic concentrations of tylosin phosphate in feed. In years one and two, no tulathromycin-resistant M. haemolytica were detected, whereas 5 isolates (0.4%) were resistant in year three. These resistant isolates were collected from three cattle originating from a single pen, were all serotype 1, and were genetically related (≥ 89% similarity) according to pulsed-field gel electrophoreses patterns. The five tulathromycin-resistant isolates were multi-drug resistant also exhibiting resistance to oxytetracycline, tilmicosin, ampicillin, or penicillin. The macrolide resistance genes erm(42), erm(A), erm(B), erm(F), erm(X) and msr(E)-mph(E), were not detected in the tulathromycin-resistant M. haemolytica. This study showed that tulathromycin resistance in M. haemolytica from a general population of feedlot cattle in western Canada was low and did not change over a three-year period after tulathromycin was approved for use in cattle. |
| first_indexed | 2024-04-12T10:16:12Z |
| format | Article |
| id | doaj.art-b82d61c69ab041a4a831b1ccd7c1f459 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T10:16:12Z |
| publishDate | 2013-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b82d61c69ab041a4a831b1ccd7c1f4592022-12-22T03:37:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2013-10-01410.3389/fmicb.2013.0029765709Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a three-year periodTrevor W. Alexander0Shaun R. Cook1Cassidy L. Klima2Ed eTopp3Tim A. McAllister4Agriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaMannheimia haemolytica isolated from feedlot cattle were tested for tulathromycin resistance. Cattle were sampled over a three-year period, starting 12 months after approval of tulathromycin for prevention and treatment of bovine respiratory disease. Nasopharyngeal samples from approximately 5,814 cattle were collected when cattle entered feedlots (N = 4) and again from the same cattle after ≥ 60 d on feed. The antimicrobial use history for each animal was recorded. Mannheimia haemolytica was isolated from 796 (13.7%) entry samples and 1,038 (20.6%) ≥ 60 d samples. Of the cattle positive for M. haemolytica, 18.5%, 2.9%, and 2.4% were administered therapeutic concentrations of tulathromycin, tilmicosin, or tylosin tartrate, respectively. In addition, 13.2% were administered subtherapeutic concentrations of tylosin phosphate in feed. In years one and two, no tulathromycin-resistant M. haemolytica were detected, whereas 5 isolates (0.4%) were resistant in year three. These resistant isolates were collected from three cattle originating from a single pen, were all serotype 1, and were genetically related (≥ 89% similarity) according to pulsed-field gel electrophoreses patterns. The five tulathromycin-resistant isolates were multi-drug resistant also exhibiting resistance to oxytetracycline, tilmicosin, ampicillin, or penicillin. The macrolide resistance genes erm(42), erm(A), erm(B), erm(F), erm(X) and msr(E)-mph(E), were not detected in the tulathromycin-resistant M. haemolytica. This study showed that tulathromycin resistance in M. haemolytica from a general population of feedlot cattle in western Canada was low and did not change over a three-year period after tulathromycin was approved for use in cattle.http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00297/fullCattleMannheimia haemolyticaantimicrobial resistancetulathromycinfeedlot |
| spellingShingle | Trevor W. Alexander Shaun R. Cook Cassidy L. Klima Ed eTopp Tim A. McAllister Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a three-year period Frontiers in Microbiology Cattle Mannheimia haemolytica antimicrobial resistance tulathromycin feedlot |
| title | Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a three-year period |
| title_full | Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a three-year period |
| title_fullStr | Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a three-year period |
| title_full_unstemmed | Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a three-year period |
| title_short | Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a three-year period |
| title_sort | susceptibility to tulathromycin in mannheimia haemolytica isolated from feedlot cattle over a three year period |
| topic | Cattle Mannheimia haemolytica antimicrobial resistance tulathromycin feedlot |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00297/full |
| work_keys_str_mv | AT trevorwalexander susceptibilitytotulathromycininmannheimiahaemolyticaisolatedfromfeedlotcattleoverathreeyearperiod AT shaunrcook susceptibilitytotulathromycininmannheimiahaemolyticaisolatedfromfeedlotcattleoverathreeyearperiod AT cassidylklima susceptibilitytotulathromycininmannheimiahaemolyticaisolatedfromfeedlotcattleoverathreeyearperiod AT edetopp susceptibilitytotulathromycininmannheimiahaemolyticaisolatedfromfeedlotcattleoverathreeyearperiod AT timamcallister susceptibilitytotulathromycininmannheimiahaemolyticaisolatedfromfeedlotcattleoverathreeyearperiod |