Association of MTHFR polymorphism, folic acid and vitamin B12 with serum homocysteine levels in pregnant women
Homocysteine is known to be associated with adverse vascular and metabolic effects, as well as pregnancy complications. Its serum levels are influenced by the function of the enzyme methylenetetrahydrofolate reductase (MTHFR) and the dietary intake of folic acid, vitamin B12, and methionine. In thi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2024-01-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/9260 |
| _version_ | 1797261388957089792 |
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| author | Ana Bošković Ana Ćuk Vedrana Mandrapa Ana Dugandžić Šimić Ivona Cvetković Martina Orlović Vlaho Tanja Krešić Tanja Tomić Vajdana Tomić |
| author_facet | Ana Bošković Ana Ćuk Vedrana Mandrapa Ana Dugandžić Šimić Ivona Cvetković Martina Orlović Vlaho Tanja Krešić Tanja Tomić Vajdana Tomić |
| author_sort | Ana Bošković |
| collection | DOAJ |
| description |
Homocysteine is known to be associated with adverse vascular and metabolic effects, as well as pregnancy complications. Its serum levels are influenced by the function of the enzyme methylenetetrahydrofolate reductase (MTHFR) and the dietary intake of folic acid, vitamin B12, and methionine. In this cross-sectional study, we investigated the association of genetic polymorphisms of the MTHFR gene with vitamin status in pregnant women during mandatory folic acid supplementation. The study included 102 pregnant women between 24 and 28 weeks of gestation who were attending regular outpatient examinations at the maternity clinic. Homocysteine, folic acid, vitamin B12 levels, and MTHFR gene polymorphisms (C677T and A1298C) were analyzed. Significant associations were found between vitamin B12 and folic acid levels with homocysteine (P < 0.001), with lower serum levels of these vitamins being associated with higher homocysteine levels. Surprisingly, there was no significant association between MTHFR genetic polymorphisms and serum homocysteine levels, likely attributed to the supplementation of folic acid and vitamin B12 in vitamin supplements for pregnant women, which counteracts the effect of the mutation. Remarkably, a high prevalence of MTHFR gene mutations was found, with the C677T polymorphism present in 56.9% and the A1298C polymorphism in 87.2% of pregnant women. These findings emphasize the importance of adequate folic acid and vitamin B12 intake during pregnancy to regulate homocysteine levels. Although the MTHFR gene mutations were highly prevalent, their influence on homocysteine levels in this population appears to be mitigated by vitamin supplementation. Further research is warranted to explore the impact of these mutations on other aspects of pregnancy outcomes. The trial is registrated at Clinicaltrail.gov (NCT04952324).
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| first_indexed | 2024-04-24T23:40:26Z |
| format | Article |
| id | doaj.art-b82d8c16c07246a58bcfaf9656b03a9b |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:40:26Z |
| publishDate | 2024-01-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-b82d8c16c07246a58bcfaf9656b03a9b2024-03-15T13:21:14ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2024-01-0124110.17305/bb.2023.9260Association of MTHFR polymorphism, folic acid and vitamin B12 with serum homocysteine levels in pregnant womenAna Bošković0https://orcid.org/0009-0006-0730-712XAna Ćuk1https://orcid.org/0000-0003-3336-6300Vedrana Mandrapa2https://orcid.org/0009-0007-7180-0610Ana Dugandžić Šimić3https://orcid.org/0000-0001-7006-4227Ivona Cvetković4https://orcid.org/0000-0001-8012-7236Martina Orlović Vlaho5https://orcid.org/0000-0002-5337-0780Tanja Krešić6https://orcid.org/0000-0001-5520-9707Tanja Tomić7https://orcid.org/0009-0009-1077-9701Vajdana Tomić8https://orcid.org/0000-0002-0469-7840Department of Obstetrics and Gynecology, University Clinical Hospital Mostar, Mostar, Bosnia and HerzegovinaDepartment of Laboratory Diagnostics, University Clinical Hospital Mostar, Mostar, Bosnia and HerzegovinaDepartment of Obstetrics and Gynecology, University Clinical Hospital Mostar, Mostar, Bosnia and HerzegovinaDepartment of Obstetrics and Gynecology, University Clinical Hospital Mostar, Mostar, Bosnia and HerzegovinaDepartment of Laboratory Diagnostics, University Clinical Hospital Mostar, Mostar, Bosnia and HerzegovinaDepartment of Obstetrics and Gynecology, University Clinical Hospital Mostar, Mostar, Bosnia and Herzegovina; Faculty of Health Studies, University Mostar, Mostar, Bosnia and Herzegovina; Faculty of Medicine, University Mostar, Mostar, Bosnia and HerzegovinaDepartment of Obstetrics and Gynecology, University Clinical Hospital Mostar, Mostar, Bosnia and HerzegovinaFaculty of Health Studies, University Mostar, Mostar, Bosnia and HerzegovinaDepartment of Obstetrics and Gynecology, University Clinical Hospital Mostar, Mostar, Bosnia and Herzegovina; Faculty of Health Studies, University Mostar, Mostar, Bosnia and Herzegovina; Faculty of Medicine, University Mostar, Mostar, Bosnia and Herzegovina Homocysteine is known to be associated with adverse vascular and metabolic effects, as well as pregnancy complications. Its serum levels are influenced by the function of the enzyme methylenetetrahydrofolate reductase (MTHFR) and the dietary intake of folic acid, vitamin B12, and methionine. In this cross-sectional study, we investigated the association of genetic polymorphisms of the MTHFR gene with vitamin status in pregnant women during mandatory folic acid supplementation. The study included 102 pregnant women between 24 and 28 weeks of gestation who were attending regular outpatient examinations at the maternity clinic. Homocysteine, folic acid, vitamin B12 levels, and MTHFR gene polymorphisms (C677T and A1298C) were analyzed. Significant associations were found between vitamin B12 and folic acid levels with homocysteine (P < 0.001), with lower serum levels of these vitamins being associated with higher homocysteine levels. Surprisingly, there was no significant association between MTHFR genetic polymorphisms and serum homocysteine levels, likely attributed to the supplementation of folic acid and vitamin B12 in vitamin supplements for pregnant women, which counteracts the effect of the mutation. Remarkably, a high prevalence of MTHFR gene mutations was found, with the C677T polymorphism present in 56.9% and the A1298C polymorphism in 87.2% of pregnant women. These findings emphasize the importance of adequate folic acid and vitamin B12 intake during pregnancy to regulate homocysteine levels. Although the MTHFR gene mutations were highly prevalent, their influence on homocysteine levels in this population appears to be mitigated by vitamin supplementation. Further research is warranted to explore the impact of these mutations on other aspects of pregnancy outcomes. The trial is registrated at Clinicaltrail.gov (NCT04952324). https://www.bjbms.org/ojs/index.php/bjbms/article/view/9260MTHFR polymorphismfolic acidvitamin B12homocisteinepregnancy |
| spellingShingle | Ana Bošković Ana Ćuk Vedrana Mandrapa Ana Dugandžić Šimić Ivona Cvetković Martina Orlović Vlaho Tanja Krešić Tanja Tomić Vajdana Tomić Association of MTHFR polymorphism, folic acid and vitamin B12 with serum homocysteine levels in pregnant women Biomolecules & Biomedicine MTHFR polymorphism folic acid vitamin B12 homocisteine pregnancy |
| title | Association of MTHFR polymorphism, folic acid and vitamin B12 with serum homocysteine levels in pregnant women |
| title_full | Association of MTHFR polymorphism, folic acid and vitamin B12 with serum homocysteine levels in pregnant women |
| title_fullStr | Association of MTHFR polymorphism, folic acid and vitamin B12 with serum homocysteine levels in pregnant women |
| title_full_unstemmed | Association of MTHFR polymorphism, folic acid and vitamin B12 with serum homocysteine levels in pregnant women |
| title_short | Association of MTHFR polymorphism, folic acid and vitamin B12 with serum homocysteine levels in pregnant women |
| title_sort | association of mthfr polymorphism folic acid and vitamin b12 with serum homocysteine levels in pregnant women |
| topic | MTHFR polymorphism folic acid vitamin B12 homocisteine pregnancy |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/9260 |
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