Antioxidant, Anti-Inflammatory Effects and Ability to Stimulate Wound Healing of a Common-Plantain Extract in Alginate Gel Formulations
Our study aimed to investigate the biological effects of a common-plantain (<i>Plantago major</i> L.) extract, encapsulated in alginate, on dermal human fibroblast cultures <i>in vitro</i>, in view of its potential use as a wound healing adjuvant therapy. Common-plantain extr...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2310-2861/9/11/901 |
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| author | Ioana Bâldea Ildiko Lung Ocsana Opriş Adina Stegarescu Irina Kacso Maria-Loredana Soran |
| author_facet | Ioana Bâldea Ildiko Lung Ocsana Opriş Adina Stegarescu Irina Kacso Maria-Loredana Soran |
| author_sort | Ioana Bâldea |
| collection | DOAJ |
| description | Our study aimed to investigate the biological effects of a common-plantain (<i>Plantago major</i> L.) extract, encapsulated in alginate, on dermal human fibroblast cultures <i>in vitro</i>, in view of its potential use as a wound healing adjuvant therapy. Common-plantain extracts were obtained by infusion and ultrasound extraction, and their total polyphenolic content and antioxidant capacity were determined by spectrophotometry. The best extract, which was obtained by infusion, was further encapsulated in sodium alginate in two different formulations. Fourier Transform Infrared Spectroscopy (FTIR) was used to demonstrate the existing interactions in the obtained common-plantain extract in the alginate formulations. The encapsulation efficiency was evaluated based on the total polyphenol content. These alginate gel formulations were further used <i>in vitro</i> to determine their biocompatibility and antioxidant and anti-inflammatory effects by spectrophotometry and ELISA, as well as their ability to stimulate fibroblast migration (scratch test assay) at different time points. In addition, the collagen 1 and 3 levels were determined by Western blot analysis. The data showed that the microencapsulated plantain extract formulations induced an antioxidant, anti-inflammatory effect, enhanced collagen production and increased wound closure in the first 8 h of their application. These results are encouraging for the use of this alginate plantain extract formulation as an adjuvant for skin wound healing. |
| first_indexed | 2024-03-09T16:49:13Z |
| format | Article |
| id | doaj.art-b82f8a30e2f74fc3ac4012398f72f682 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T16:49:13Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
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| series | Gels |
| spelling | doaj.art-b82f8a30e2f74fc3ac4012398f72f6822023-11-24T14:43:27ZengMDPI AGGels2310-28612023-11-0191190110.3390/gels9110901Antioxidant, Anti-Inflammatory Effects and Ability to Stimulate Wound Healing of a Common-Plantain Extract in Alginate Gel FormulationsIoana Bâldea0Ildiko Lung1Ocsana Opriş2Adina Stegarescu3Irina Kacso4Maria-Loredana Soran5Department of Physiology, Iuliu Haţieganu University of Medicine and Pharmacy, Clinicilor 1, 400006 Cluj-Napoca, RomaniaNational Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat, 400293 Cluj-Napoca, RomaniaNational Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat, 400293 Cluj-Napoca, RomaniaNational Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat, 400293 Cluj-Napoca, RomaniaNational Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat, 400293 Cluj-Napoca, RomaniaNational Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat, 400293 Cluj-Napoca, RomaniaOur study aimed to investigate the biological effects of a common-plantain (<i>Plantago major</i> L.) extract, encapsulated in alginate, on dermal human fibroblast cultures <i>in vitro</i>, in view of its potential use as a wound healing adjuvant therapy. Common-plantain extracts were obtained by infusion and ultrasound extraction, and their total polyphenolic content and antioxidant capacity were determined by spectrophotometry. The best extract, which was obtained by infusion, was further encapsulated in sodium alginate in two different formulations. Fourier Transform Infrared Spectroscopy (FTIR) was used to demonstrate the existing interactions in the obtained common-plantain extract in the alginate formulations. The encapsulation efficiency was evaluated based on the total polyphenol content. These alginate gel formulations were further used <i>in vitro</i> to determine their biocompatibility and antioxidant and anti-inflammatory effects by spectrophotometry and ELISA, as well as their ability to stimulate fibroblast migration (scratch test assay) at different time points. In addition, the collagen 1 and 3 levels were determined by Western blot analysis. The data showed that the microencapsulated plantain extract formulations induced an antioxidant, anti-inflammatory effect, enhanced collagen production and increased wound closure in the first 8 h of their application. These results are encouraging for the use of this alginate plantain extract formulation as an adjuvant for skin wound healing.https://www.mdpi.com/2310-2861/9/11/901alginate beadsanti-inflammatory effectantioxidant capacityplantainpolyphenolswound healing |
| spellingShingle | Ioana Bâldea Ildiko Lung Ocsana Opriş Adina Stegarescu Irina Kacso Maria-Loredana Soran Antioxidant, Anti-Inflammatory Effects and Ability to Stimulate Wound Healing of a Common-Plantain Extract in Alginate Gel Formulations Gels alginate beads anti-inflammatory effect antioxidant capacity plantain polyphenols wound healing |
| title | Antioxidant, Anti-Inflammatory Effects and Ability to Stimulate Wound Healing of a Common-Plantain Extract in Alginate Gel Formulations |
| title_full | Antioxidant, Anti-Inflammatory Effects and Ability to Stimulate Wound Healing of a Common-Plantain Extract in Alginate Gel Formulations |
| title_fullStr | Antioxidant, Anti-Inflammatory Effects and Ability to Stimulate Wound Healing of a Common-Plantain Extract in Alginate Gel Formulations |
| title_full_unstemmed | Antioxidant, Anti-Inflammatory Effects and Ability to Stimulate Wound Healing of a Common-Plantain Extract in Alginate Gel Formulations |
| title_short | Antioxidant, Anti-Inflammatory Effects and Ability to Stimulate Wound Healing of a Common-Plantain Extract in Alginate Gel Formulations |
| title_sort | antioxidant anti inflammatory effects and ability to stimulate wound healing of a common plantain extract in alginate gel formulations |
| topic | alginate beads anti-inflammatory effect antioxidant capacity plantain polyphenols wound healing |
| url | https://www.mdpi.com/2310-2861/9/11/901 |
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