Can selenium‐enriched spirulina supplementation ameliorate sepsis outcomes in selenium‐deficient animals?
Abstract In intensive care units, sepsis is the first cause of death. In this pathology, inflammation and oxidative status play a crucial role in patient outcomes. Interestingly, 92% of septic patients exhibit low selenium plasma concentrations (a component of antioxidant enzymes). Moreover, Spiruli...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-07-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.14933 |
| _version_ | 1818736898400583680 |
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| author | Thomas Castel Michaël Theron Karine Pichavant‐Rafini Anthony Guernec Aurélie Joublin‐Delavat Bleuenn Gueguen Karelle Leon |
| author_facet | Thomas Castel Michaël Theron Karine Pichavant‐Rafini Anthony Guernec Aurélie Joublin‐Delavat Bleuenn Gueguen Karelle Leon |
| author_sort | Thomas Castel |
| collection | DOAJ |
| description | Abstract In intensive care units, sepsis is the first cause of death. In this pathology, inflammation and oxidative status play a crucial role in patient outcomes. Interestingly, 92% of septic patients exhibit low selenium plasma concentrations (a component of antioxidant enzymes). Moreover, Spirulina platensis, a blue‐green algae, demonstrated anti‐inflammatory effects. In this context, the main purpose of our study was to analyze the effect of a selenium‐enriched spirulina after a selenium deficiency on sepsis outcome in rats. Sixty‐four rats were fed 12 weeks with a selenium‐deficient food. After 8 weeks, rats were supplemented (via drinking water) for 4 weeks with sodium selenite (Se), spirulina (Spi), or selenium‐enriched spirulina (SeSp). Sepsis was then induced by cecal ligature and puncture, and survival duration was observed. The plasma selenium concentration was measured by ICPMS. Expression of GPx1 and GPx3 mRNA was measured by RT‐PCR. Blood parameters (lactates and HCO3− concentrations, pH, PO2, and PCO2) were analyzed at 0, 1, and 2 h as well as inflammatory cytokines (IL‐6, TNF‐α, IL‐10). Sodium selenite and SeSP supplementations restored plasma selenium concentration prior to sepsis. The survival duration of SeSP septic rats was significantly lower than that of selenium‐supplemented ones. Gpx1 mRNA was increased after a selenium‐enriched spirulina supplementation while Gpx3 mRNA levels remained unchanged. Furthermore, sodium selenite prevented sepsis‐induced acidosis. Our results show that on a basis of a Se deficiency, selenium‐enriched spirulina supplementations significantly worsen sepsis outcome when compared to Se supplementation. Furthermore, Se supplementation but not selenium‐enriched spirulina supplementation decreased inflammation and restored acid–base equilibrium after a sepsis induction. |
| first_indexed | 2024-12-18T00:44:28Z |
| format | Article |
| id | doaj.art-b83571df66a340e09d9ed1ee25c782ad |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-12-18T00:44:28Z |
| publishDate | 2021-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-b83571df66a340e09d9ed1ee25c782ad2022-12-21T21:26:47ZengWileyPhysiological Reports2051-817X2021-07-01914n/an/a10.14814/phy2.14933Can selenium‐enriched spirulina supplementation ameliorate sepsis outcomes in selenium‐deficient animals?Thomas Castel0Michaël Theron1Karine Pichavant‐Rafini2Anthony Guernec3Aurélie Joublin‐Delavat4Bleuenn Gueguen5Karelle Leon6Université de BrestEA 4324 ORPHYUFR Sciences et Techniques Brest FranceUniversité de BrestEA 4324 ORPHYUFR Sciences et Techniques Brest FranceUniversité de BrestEA 4324 ORPHYUFR Sciences et Techniques Brest FranceUniversité de BrestEA 4324 ORPHYUFR Sciences et Techniques Brest FranceUniversité de BrestEA 4324 ORPHYUFR Sciences et Techniques Brest FranceCNRSUniv BrestUMS 3113 Plouzané FranceUniversité de BrestEA 4324 ORPHYUFR Sciences et Techniques Brest FranceAbstract In intensive care units, sepsis is the first cause of death. In this pathology, inflammation and oxidative status play a crucial role in patient outcomes. Interestingly, 92% of septic patients exhibit low selenium plasma concentrations (a component of antioxidant enzymes). Moreover, Spirulina platensis, a blue‐green algae, demonstrated anti‐inflammatory effects. In this context, the main purpose of our study was to analyze the effect of a selenium‐enriched spirulina after a selenium deficiency on sepsis outcome in rats. Sixty‐four rats were fed 12 weeks with a selenium‐deficient food. After 8 weeks, rats were supplemented (via drinking water) for 4 weeks with sodium selenite (Se), spirulina (Spi), or selenium‐enriched spirulina (SeSp). Sepsis was then induced by cecal ligature and puncture, and survival duration was observed. The plasma selenium concentration was measured by ICPMS. Expression of GPx1 and GPx3 mRNA was measured by RT‐PCR. Blood parameters (lactates and HCO3− concentrations, pH, PO2, and PCO2) were analyzed at 0, 1, and 2 h as well as inflammatory cytokines (IL‐6, TNF‐α, IL‐10). Sodium selenite and SeSP supplementations restored plasma selenium concentration prior to sepsis. The survival duration of SeSP septic rats was significantly lower than that of selenium‐supplemented ones. Gpx1 mRNA was increased after a selenium‐enriched spirulina supplementation while Gpx3 mRNA levels remained unchanged. Furthermore, sodium selenite prevented sepsis‐induced acidosis. Our results show that on a basis of a Se deficiency, selenium‐enriched spirulina supplementations significantly worsen sepsis outcome when compared to Se supplementation. Furthermore, Se supplementation but not selenium‐enriched spirulina supplementation decreased inflammation and restored acid–base equilibrium after a sepsis induction.https://doi.org/10.14814/phy2.14933GPxlactatesseleniumsepsisSpirulinasupplementation |
| spellingShingle | Thomas Castel Michaël Theron Karine Pichavant‐Rafini Anthony Guernec Aurélie Joublin‐Delavat Bleuenn Gueguen Karelle Leon Can selenium‐enriched spirulina supplementation ameliorate sepsis outcomes in selenium‐deficient animals? Physiological Reports GPx lactates selenium sepsis Spirulina supplementation |
| title | Can selenium‐enriched spirulina supplementation ameliorate sepsis outcomes in selenium‐deficient animals? |
| title_full | Can selenium‐enriched spirulina supplementation ameliorate sepsis outcomes in selenium‐deficient animals? |
| title_fullStr | Can selenium‐enriched spirulina supplementation ameliorate sepsis outcomes in selenium‐deficient animals? |
| title_full_unstemmed | Can selenium‐enriched spirulina supplementation ameliorate sepsis outcomes in selenium‐deficient animals? |
| title_short | Can selenium‐enriched spirulina supplementation ameliorate sepsis outcomes in selenium‐deficient animals? |
| title_sort | can selenium enriched spirulina supplementation ameliorate sepsis outcomes in selenium deficient animals |
| topic | GPx lactates selenium sepsis Spirulina supplementation |
| url | https://doi.org/10.14814/phy2.14933 |
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