Rhein attenuates inflammation through inhibition of NF-κB and NALP3 inflammasome in vivo and in vitro
Hui Ge,1,* Hao Tang,2,* Yanbing Liang,2 Jingguo Wu,2 Qing Yang,2 Lijin Zeng,2 Zhongfu Ma2 1Department of Health Care Clinic, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; 2Department of General Internal Medicine, the First Affiliated Hospital, Sun Yat-sen University, Guan...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2017-06-01
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| Series: | Drug Design, Development and Therapy |
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| Online Access: | https://www.dovepress.com/rhein-attenuates-inflammation-through-inhibition-of-nf-kappab-and-nalp-peer-reviewed-article-DDDT |
| _version_ | 1818481974099050496 |
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| author | Ge H Tang H Liang Y Wu J Yang Q Zeng L Ma Z |
| author_facet | Ge H Tang H Liang Y Wu J Yang Q Zeng L Ma Z |
| author_sort | Ge H |
| collection | DOAJ |
| description | Hui Ge,1,* Hao Tang,2,* Yanbing Liang,2 Jingguo Wu,2 Qing Yang,2 Lijin Zeng,2 Zhongfu Ma2 1Department of Health Care Clinic, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; 2Department of General Internal Medicine, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China *These authors contributed equally to this work Abstract: Rhein is an important component in traditional Chinese herbal medicine formulations for gastrointestinal disorders, including inflammatory bowel diseases such as ulcerative colitis. In this study, we investigated the beneficial effects of rhein in inflammation models in the transgenic zebrafish line TG (corolla eGFP), in which both macrophages and neutrophils express eGFP and RAW264.7 macrophages. We found that the tail-cutting-induced migration of immune cells was significantly reduced in transgenic zebrafish treated with rhein. In addition, the production of proinflammatory cytokines, including IL-6, IL-1β, and tumor necrosis factor-α, were significantly reduced in lipopolysaccharide (LPS)-induced RAW264.7 macrophages treated with rhein. Parallel to the inhibition of proinflammatory cytokines, rhein significantly reduced phosphorylation levels of NF-κB p65 and inducible nitric oxide synthase, as well as COX-2 protein expression levels. Furthermore, rhein significantly reduced NALP3 and cleaved IL-1β expression in LPS + ATP-induced RAW264.7 macrophages. Thus, the present study demonstrates that rhein may exhibit its anti-inflammatory action via inhibition of NF-κB and NALP3 inflammasome pathways. Keywords: rhein, inflammatory, zebrafish, NF-κB, iNOS, COX-2, NALP3 |
| first_indexed | 2024-12-10T11:42:08Z |
| format | Article |
| id | doaj.art-b836744fda61401d9f1c61038b289076 |
| institution | Directory Open Access Journal |
| issn | 1177-8881 |
| language | English |
| last_indexed | 2024-12-10T11:42:08Z |
| publishDate | 2017-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Drug Design, Development and Therapy |
| spelling | doaj.art-b836744fda61401d9f1c61038b2890762022-12-22T01:50:12ZengDove Medical PressDrug Design, Development and Therapy1177-88812017-06-01Volume 111663167133157Rhein attenuates inflammation through inhibition of NF-κB and NALP3 inflammasome in vivo and in vitroGe HTang HLiang YWu JYang QZeng LMa ZHui Ge,1,* Hao Tang,2,* Yanbing Liang,2 Jingguo Wu,2 Qing Yang,2 Lijin Zeng,2 Zhongfu Ma2 1Department of Health Care Clinic, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; 2Department of General Internal Medicine, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China *These authors contributed equally to this work Abstract: Rhein is an important component in traditional Chinese herbal medicine formulations for gastrointestinal disorders, including inflammatory bowel diseases such as ulcerative colitis. In this study, we investigated the beneficial effects of rhein in inflammation models in the transgenic zebrafish line TG (corolla eGFP), in which both macrophages and neutrophils express eGFP and RAW264.7 macrophages. We found that the tail-cutting-induced migration of immune cells was significantly reduced in transgenic zebrafish treated with rhein. In addition, the production of proinflammatory cytokines, including IL-6, IL-1β, and tumor necrosis factor-α, were significantly reduced in lipopolysaccharide (LPS)-induced RAW264.7 macrophages treated with rhein. Parallel to the inhibition of proinflammatory cytokines, rhein significantly reduced phosphorylation levels of NF-κB p65 and inducible nitric oxide synthase, as well as COX-2 protein expression levels. Furthermore, rhein significantly reduced NALP3 and cleaved IL-1β expression in LPS + ATP-induced RAW264.7 macrophages. Thus, the present study demonstrates that rhein may exhibit its anti-inflammatory action via inhibition of NF-κB and NALP3 inflammasome pathways. Keywords: rhein, inflammatory, zebrafish, NF-κB, iNOS, COX-2, NALP3https://www.dovepress.com/rhein-attenuates-inflammation-through-inhibition-of-nf-kappab-and-nalp-peer-reviewed-article-DDDTRheinInflammatoryZebrafishNF-κ BiNOSCOX-2NALP3. |
| spellingShingle | Ge H Tang H Liang Y Wu J Yang Q Zeng L Ma Z Rhein attenuates inflammation through inhibition of NF-κB and NALP3 inflammasome in vivo and in vitro Drug Design, Development and Therapy Rhein Inflammatory Zebrafish NF-κ B iNOS COX-2 NALP3. |
| title | Rhein attenuates inflammation through inhibition of NF-κB and NALP3 inflammasome in vivo and in vitro |
| title_full | Rhein attenuates inflammation through inhibition of NF-κB and NALP3 inflammasome in vivo and in vitro |
| title_fullStr | Rhein attenuates inflammation through inhibition of NF-κB and NALP3 inflammasome in vivo and in vitro |
| title_full_unstemmed | Rhein attenuates inflammation through inhibition of NF-κB and NALP3 inflammasome in vivo and in vitro |
| title_short | Rhein attenuates inflammation through inhibition of NF-κB and NALP3 inflammasome in vivo and in vitro |
| title_sort | rhein attenuates inflammation through inhibition of nf kappa b and nalp3 inflammasome in vivo and in vitro |
| topic | Rhein Inflammatory Zebrafish NF-κ B iNOS COX-2 NALP3. |
| url | https://www.dovepress.com/rhein-attenuates-inflammation-through-inhibition-of-nf-kappab-and-nalp-peer-reviewed-article-DDDT |
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