In Vitro Evaluation of the Therapeutic Potential of Phage VA7 against Enterotoxigenic <i>Bacteroides fragilis</i> Infection
Since the beginning of the 20th century, bacteriophages (phages), i.e., viruses that infect bacteria, have been used as antimicrobial agents for treating various infections. Phage preparations targeting a number of bacterial pathogens are still in use in the post-Soviet states and are experiencing a...
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2021-10-01
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| author | Nata Bakuradze Maya Merabishvili Khatuna Makalatia Elene Kakabadze Nino Grdzelishvili Jeroen Wagemans Cedric Lood Irakli Chachua Mario Vaneechoutte Rob Lavigne Jean-Paul Pirnay Ivane Abiatari Nina Chanishvili |
| author_facet | Nata Bakuradze Maya Merabishvili Khatuna Makalatia Elene Kakabadze Nino Grdzelishvili Jeroen Wagemans Cedric Lood Irakli Chachua Mario Vaneechoutte Rob Lavigne Jean-Paul Pirnay Ivane Abiatari Nina Chanishvili |
| author_sort | Nata Bakuradze |
| collection | DOAJ |
| description | Since the beginning of the 20th century, bacteriophages (phages), i.e., viruses that infect bacteria, have been used as antimicrobial agents for treating various infections. Phage preparations targeting a number of bacterial pathogens are still in use in the post-Soviet states and are experiencing a revival in the Western world. However, phages have never been used to treat diseases caused by <i>Bacteroides fragilis</i>, the leading agent cultured in anaerobic abscesses and postoperative peritonitis. Enterotoxin-producing strains of <i>B. fragilis</i> have been associated with the development of inflammatory diarrhea and colorectal carcinoma. In this study, we evaluated the molecular biosafety and antimicrobial properties of novel phage species vB_BfrS_VA7 (VA7) lysate, as well as its impact on cytokine IL-8 production in an enterotoxigenic <i>B. fragilis</i> (ETBF)-infected colonic epithelial cell (CEC) culture model. Compared to untreated infected cells, the addition of phage VA7 to ETBF-infected CECs led to significantly reduced bacterial counts and IL-8 levels. This in vitro study confirms the potential of phage VA7 as an antibacterial agent for use in prophylaxis or in the treatment of <i>B. fragilis</i> infections and associated colorectal carcinoma. |
| first_indexed | 2024-03-10T06:08:47Z |
| format | Article |
| id | doaj.art-b83cc166cdbc4b67aac01ece46b188e0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T06:08:47Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-b83cc166cdbc4b67aac01ece46b188e02023-11-22T20:19:58ZengMDPI AGViruses1999-49152021-10-011310204410.3390/v13102044In Vitro Evaluation of the Therapeutic Potential of Phage VA7 against Enterotoxigenic <i>Bacteroides fragilis</i> InfectionNata Bakuradze0Maya Merabishvili1Khatuna Makalatia2Elene Kakabadze3Nino Grdzelishvili4Jeroen Wagemans5Cedric Lood6Irakli Chachua7Mario Vaneechoutte8Rob Lavigne9Jean-Paul Pirnay10Ivane Abiatari11Nina Chanishvili12Research & Development Department, George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi 0160, GeorgiaResearch & Development Department, George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi 0160, GeorgiaResearch & Development Department, George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi 0160, GeorgiaResearch & Development Department, George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi 0160, GeorgiaResearch & Development Department, George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi 0160, GeorgiaLaboratory of Gene Technology, Department of Biosystems, KU Leuven, 3001 Leuven, BelgiumLaboratory of Gene Technology, Department of Biosystems, KU Leuven, 3001 Leuven, BelgiumInstitute of Medical and Public Health Research, IIia State University, Tbilisi 0162, GeorgiaLaboratory Bacteriology Research, Ghent University, 9000 Ghent, BelgiumLaboratory of Gene Technology, Department of Biosystems, KU Leuven, 3001 Leuven, BelgiumLaboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, 1120 Brussels, BelgiumInstitute of Medical and Public Health Research, IIia State University, Tbilisi 0162, GeorgiaResearch & Development Department, George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi 0160, GeorgiaSince the beginning of the 20th century, bacteriophages (phages), i.e., viruses that infect bacteria, have been used as antimicrobial agents for treating various infections. Phage preparations targeting a number of bacterial pathogens are still in use in the post-Soviet states and are experiencing a revival in the Western world. However, phages have never been used to treat diseases caused by <i>Bacteroides fragilis</i>, the leading agent cultured in anaerobic abscesses and postoperative peritonitis. Enterotoxin-producing strains of <i>B. fragilis</i> have been associated with the development of inflammatory diarrhea and colorectal carcinoma. In this study, we evaluated the molecular biosafety and antimicrobial properties of novel phage species vB_BfrS_VA7 (VA7) lysate, as well as its impact on cytokine IL-8 production in an enterotoxigenic <i>B. fragilis</i> (ETBF)-infected colonic epithelial cell (CEC) culture model. Compared to untreated infected cells, the addition of phage VA7 to ETBF-infected CECs led to significantly reduced bacterial counts and IL-8 levels. This in vitro study confirms the potential of phage VA7 as an antibacterial agent for use in prophylaxis or in the treatment of <i>B. fragilis</i> infections and associated colorectal carcinoma.https://www.mdpi.com/1999-4915/13/10/2044bacteriophagesphage therapyenterotoxigenic <i>Bacteroides fragilis</i>ETBFin vitro modelcolorectal carcinoma |
| spellingShingle | Nata Bakuradze Maya Merabishvili Khatuna Makalatia Elene Kakabadze Nino Grdzelishvili Jeroen Wagemans Cedric Lood Irakli Chachua Mario Vaneechoutte Rob Lavigne Jean-Paul Pirnay Ivane Abiatari Nina Chanishvili In Vitro Evaluation of the Therapeutic Potential of Phage VA7 against Enterotoxigenic <i>Bacteroides fragilis</i> Infection Viruses bacteriophages phage therapy enterotoxigenic <i>Bacteroides fragilis</i> ETBF in vitro model colorectal carcinoma |
| title | In Vitro Evaluation of the Therapeutic Potential of Phage VA7 against Enterotoxigenic <i>Bacteroides fragilis</i> Infection |
| title_full | In Vitro Evaluation of the Therapeutic Potential of Phage VA7 against Enterotoxigenic <i>Bacteroides fragilis</i> Infection |
| title_fullStr | In Vitro Evaluation of the Therapeutic Potential of Phage VA7 against Enterotoxigenic <i>Bacteroides fragilis</i> Infection |
| title_full_unstemmed | In Vitro Evaluation of the Therapeutic Potential of Phage VA7 against Enterotoxigenic <i>Bacteroides fragilis</i> Infection |
| title_short | In Vitro Evaluation of the Therapeutic Potential of Phage VA7 against Enterotoxigenic <i>Bacteroides fragilis</i> Infection |
| title_sort | in vitro evaluation of the therapeutic potential of phage va7 against enterotoxigenic i bacteroides fragilis i infection |
| topic | bacteriophages phage therapy enterotoxigenic <i>Bacteroides fragilis</i> ETBF in vitro model colorectal carcinoma |
| url | https://www.mdpi.com/1999-4915/13/10/2044 |
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