Agmatine ameliorates diabetes type 2-induced nephropathy in rats
Objective: To assess the nephroprotective potential of agmatine in a rat model of streptozotocin-induced diabetic nephropathy. Methods: A single dose of streptozotocin (40 mg/kg) coupled with a fructose diet induced diabetes in Wistar rats. Agmatine (40 and 80 mg/kg) was administered to rats for 12...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=1;spage=8;epage=16;aulast=Kamel |
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| author | Fatemah O Kamel Ohoud Shagroud Mai A.Alim A.Sattar Ahmad Gamal S Abd El-Aziz Abdulhadi S Burzangi Duaa Bakhshwin Maha Jamal Shahid Karim |
| author_facet | Fatemah O Kamel Ohoud Shagroud Mai A.Alim A.Sattar Ahmad Gamal S Abd El-Aziz Abdulhadi S Burzangi Duaa Bakhshwin Maha Jamal Shahid Karim |
| author_sort | Fatemah O Kamel |
| collection | DOAJ |
| description | Objective: To assess the nephroprotective potential of agmatine in a rat model of streptozotocin-induced diabetic nephropathy.
Methods: A single dose of streptozotocin (40 mg/kg) coupled with a fructose diet induced diabetes in Wistar rats. Agmatine (40 and 80 mg/kg) was administered to rats for 12 weeks. The body weight and fasting blood glucose were measured weekly. Insulin level, urine output, total protein, albumin, blood urea nitrogen, creatinine, and cystatin-C were also determined at the end of the experiment. Furthermore, superoxide dismutase, glutathione, interleukin-1β, interleukin-6, and tumor necrosis factor-alpha were evaluated in kidney tissue. Histopathological study was also performed using hematoxylin and eosin staining.
Results: Agmatine at both doses significantly increased final body weight, and lowered fasting blood glucose, urine output, insulin, total protein, albumin, blood urea nitrogen, creatinine, and cystatin-C levels compared with the diabetic group (P < 0.05). Inflammatory markers and antioxidant effect were significantly improved in agmatine-treated rats. Moreover, the histopathological changes in renal structure were ameliorated by agmatine treatment.
Conclusions: Agmatine alleviates diabetic nephropathy by improving renal functions and reducing inflammation and oxidative stress. The molecular mechanisms of its nephroprotective actions need to be investigated in future study. |
| first_indexed | 2024-04-24T19:43:21Z |
| format | Article |
| id | doaj.art-b83d1f9b0f7e42dfb2db41f787a82185 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-04-24T19:43:21Z |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-b83d1f9b0f7e42dfb2db41f787a821852024-03-25T10:07:16ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222024-01-0114181610.4103/2221-1691.393580Agmatine ameliorates diabetes type 2-induced nephropathy in ratsFatemah O KamelOhoud ShagroudMai A.Alim A.Sattar AhmadGamal S Abd El-AzizAbdulhadi S BurzangiDuaa BakhshwinMaha JamalShahid KarimObjective: To assess the nephroprotective potential of agmatine in a rat model of streptozotocin-induced diabetic nephropathy. Methods: A single dose of streptozotocin (40 mg/kg) coupled with a fructose diet induced diabetes in Wistar rats. Agmatine (40 and 80 mg/kg) was administered to rats for 12 weeks. The body weight and fasting blood glucose were measured weekly. Insulin level, urine output, total protein, albumin, blood urea nitrogen, creatinine, and cystatin-C were also determined at the end of the experiment. Furthermore, superoxide dismutase, glutathione, interleukin-1β, interleukin-6, and tumor necrosis factor-alpha were evaluated in kidney tissue. Histopathological study was also performed using hematoxylin and eosin staining. Results: Agmatine at both doses significantly increased final body weight, and lowered fasting blood glucose, urine output, insulin, total protein, albumin, blood urea nitrogen, creatinine, and cystatin-C levels compared with the diabetic group (P < 0.05). Inflammatory markers and antioxidant effect were significantly improved in agmatine-treated rats. Moreover, the histopathological changes in renal structure were ameliorated by agmatine treatment. Conclusions: Agmatine alleviates diabetic nephropathy by improving renal functions and reducing inflammation and oxidative stress. The molecular mechanisms of its nephroprotective actions need to be investigated in future study.http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=1;spage=8;epage=16;aulast=Kamelagmatine; type 2 diabetes; nephropathy; oxidative stress; nephroprotection |
| spellingShingle | Fatemah O Kamel Ohoud Shagroud Mai A.Alim A.Sattar Ahmad Gamal S Abd El-Aziz Abdulhadi S Burzangi Duaa Bakhshwin Maha Jamal Shahid Karim Agmatine ameliorates diabetes type 2-induced nephropathy in rats Asian Pacific Journal of Tropical Biomedicine agmatine; type 2 diabetes; nephropathy; oxidative stress; nephroprotection |
| title | Agmatine ameliorates diabetes type 2-induced nephropathy in rats |
| title_full | Agmatine ameliorates diabetes type 2-induced nephropathy in rats |
| title_fullStr | Agmatine ameliorates diabetes type 2-induced nephropathy in rats |
| title_full_unstemmed | Agmatine ameliorates diabetes type 2-induced nephropathy in rats |
| title_short | Agmatine ameliorates diabetes type 2-induced nephropathy in rats |
| title_sort | agmatine ameliorates diabetes type 2 induced nephropathy in rats |
| topic | agmatine; type 2 diabetes; nephropathy; oxidative stress; nephroprotection |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2024;volume=14;issue=1;spage=8;epage=16;aulast=Kamel |
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