Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing
Existing treatment methods encounter difficulties in effectively promoting skin wound healing, making this a serious challenge for clinical treatment. Extracellular vesicles (EVs) secreted by stem cells have been proven to contribute to the regeneration and repair of wound tissue, but they cannot be...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/11/6289 |
| _version_ | 1797493054875107328 |
|---|---|
| author | Lanju Xu Yufei Liu Lizong Tang Hui Xiao Zhuo Yang Shufang Wang |
| author_facet | Lanju Xu Yufei Liu Lizong Tang Hui Xiao Zhuo Yang Shufang Wang |
| author_sort | Lanju Xu |
| collection | DOAJ |
| description | Existing treatment methods encounter difficulties in effectively promoting skin wound healing, making this a serious challenge for clinical treatment. Extracellular vesicles (EVs) secreted by stem cells have been proven to contribute to the regeneration and repair of wound tissue, but they cannot be targeted and sustained, which seriously limits their current therapeutic potential. The recombinant human collagen III protein (rhCol III) has the advantages of good water solubility, an absence of hidden viral dangers, a low rejection rate and a stable production process. In order to achieve a site-specific sustained release of EVs, we prepared a rhCol III hydrogel by cross-linking with transglutaminase (TGase) from <i>Streptomyces mobaraensis</i>, which has a uniform pore size and good biocompatibility. The release profile of the rhCol III-EVs hydrogel confirmed that the rhCol III hydrogel could slowly release EVs into the external environment. Herein, the rhCol III-EVs hydrogel effectively promoted macrophage changing from type M1 to type M2, the migration ability of L929 cells and the angiogenesis of human umbilical vein endothelial cells (HUVECs). Furthermore, the rhCol III-EVs hydrogel is shown to promote wound healing by inhibiting the inflammatory response and promoting cell proliferation and angiogenesis in a diabetic rat skin injury model. The reported results indicate that the rhCol III-EVs hydrogel could be used as a new biological material for EV delivery, and has a significant application value in skin wound healing. |
| first_indexed | 2024-03-10T01:14:30Z |
| format | Article |
| id | doaj.art-b83d7d9199db437fb921d582f5a446e5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:14:30Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b83d7d9199db437fb921d582f5a446e52023-11-23T14:12:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012311628910.3390/ijms23116289Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound HealingLanju Xu0Yufei Liu1Lizong Tang2Hui Xiao3Zhuo Yang4Shufang Wang5Key Laboratory of Bioactive Materials for Ministry of Education, College of Medicine Sciences, Nankai University, Tianjin 300071, ChinaKey Laboratory of Bioactive Materials for Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaKey Laboratory of Bioactive Materials for Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaKey Laboratory of Bioactive Materials for Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaKey Laboratory of Bioactive Materials for Ministry of Education, College of Medicine Sciences, Nankai University, Tianjin 300071, ChinaKey Laboratory of Bioactive Materials for Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaExisting treatment methods encounter difficulties in effectively promoting skin wound healing, making this a serious challenge for clinical treatment. Extracellular vesicles (EVs) secreted by stem cells have been proven to contribute to the regeneration and repair of wound tissue, but they cannot be targeted and sustained, which seriously limits their current therapeutic potential. The recombinant human collagen III protein (rhCol III) has the advantages of good water solubility, an absence of hidden viral dangers, a low rejection rate and a stable production process. In order to achieve a site-specific sustained release of EVs, we prepared a rhCol III hydrogel by cross-linking with transglutaminase (TGase) from <i>Streptomyces mobaraensis</i>, which has a uniform pore size and good biocompatibility. The release profile of the rhCol III-EVs hydrogel confirmed that the rhCol III hydrogel could slowly release EVs into the external environment. Herein, the rhCol III-EVs hydrogel effectively promoted macrophage changing from type M1 to type M2, the migration ability of L929 cells and the angiogenesis of human umbilical vein endothelial cells (HUVECs). Furthermore, the rhCol III-EVs hydrogel is shown to promote wound healing by inhibiting the inflammatory response and promoting cell proliferation and angiogenesis in a diabetic rat skin injury model. The reported results indicate that the rhCol III-EVs hydrogel could be used as a new biological material for EV delivery, and has a significant application value in skin wound healing.https://www.mdpi.com/1422-0067/23/11/6289recombinant human collagen Ⅲ proteinextracellular vesicleshydrogelswound healing |
| spellingShingle | Lanju Xu Yufei Liu Lizong Tang Hui Xiao Zhuo Yang Shufang Wang Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing International Journal of Molecular Sciences recombinant human collagen Ⅲ protein extracellular vesicles hydrogels wound healing |
| title | Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing |
| title_full | Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing |
| title_fullStr | Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing |
| title_full_unstemmed | Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing |
| title_short | Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing |
| title_sort | preparation of recombinant human collagen iii protein hydrogels with sustained release of extracellular vesicles for skin wound healing |
| topic | recombinant human collagen Ⅲ protein extracellular vesicles hydrogels wound healing |
| url | https://www.mdpi.com/1422-0067/23/11/6289 |
| work_keys_str_mv | AT lanjuxu preparationofrecombinanthumancollageniiiproteinhydrogelswithsustainedreleaseofextracellularvesiclesforskinwoundhealing AT yufeiliu preparationofrecombinanthumancollageniiiproteinhydrogelswithsustainedreleaseofextracellularvesiclesforskinwoundhealing AT lizongtang preparationofrecombinanthumancollageniiiproteinhydrogelswithsustainedreleaseofextracellularvesiclesforskinwoundhealing AT huixiao preparationofrecombinanthumancollageniiiproteinhydrogelswithsustainedreleaseofextracellularvesiclesforskinwoundhealing AT zhuoyang preparationofrecombinanthumancollageniiiproteinhydrogelswithsustainedreleaseofextracellularvesiclesforskinwoundhealing AT shufangwang preparationofrecombinanthumancollageniiiproteinhydrogelswithsustainedreleaseofextracellularvesiclesforskinwoundhealing |