Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle <i>Lepas anatifera</i>
We focus on the stalked goose barnacle <i>L. anatifera</i> adhesive system, an opportunistic less selective species for the substrate, found attached to a variety of floating objects at seas. Adhesion is an adaptative character in barnacles, ensuring adequate positioning in the habitat f...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/7/3370 |
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| author | Dany Domínguez-Pérez Daniela Almeida Josef Wissing André M. Machado Lothar Jänsch Agostinho Antunes Luís Filipe Castro Vitor Vasconcelos Alexandre Campos Isabel Cunha |
| author_facet | Dany Domínguez-Pérez Daniela Almeida Josef Wissing André M. Machado Lothar Jänsch Agostinho Antunes Luís Filipe Castro Vitor Vasconcelos Alexandre Campos Isabel Cunha |
| author_sort | Dany Domínguez-Pérez |
| collection | DOAJ |
| description | We focus on the stalked goose barnacle <i>L. anatifera</i> adhesive system, an opportunistic less selective species for the substrate, found attached to a variety of floating objects at seas. Adhesion is an adaptative character in barnacles, ensuring adequate positioning in the habitat for feeding and reproduction. The protein composition of the cement multicomplex and adhesive gland was quantitatively studied using shotgun proteomic analysis. Overall, 11,795 peptide sequences were identified in the gland and 2206 in the cement, clustered in 1689 and 217 proteinGroups, respectively. Cement specific adhesive proteins (CPs), proteases, protease inhibitors, cuticular and structural proteins, chemical cues, and many unannotated proteins were found, among others. In the cement, CPs were the most abundant (80.5%), being the bulk proteins CP100k and -52k the most expressed of all, and CP43k-like the most expressed interfacial protein. Unannotated proteins comprised 4.7% of the cement proteome, ranking several of them among the most highly expressed. Eight of these proteins showed similar physicochemical properties and amino acid composition to known CPs and classified through Principal Components Analysis (PCA) as new CPs. The importance of PCA on the identification of unannotated non-conserved adhesive proteins, whose selective pressure is on their relative amino acid abundance, was demonstrated. |
| first_indexed | 2024-03-10T12:54:30Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:54:30Z |
| publishDate | 2021-03-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b83f455ea6ad4f55ad1b12a87a3299172023-11-21T12:01:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01227337010.3390/ijms22073370Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle <i>Lepas anatifera</i>Dany Domínguez-Pérez0Daniela Almeida1Josef Wissing2André M. Machado3Lothar Jänsch4Agostinho Antunes5Luís Filipe Castro6Vitor Vasconcelos7Alexandre Campos8Isabel Cunha9CIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCellular Proteomics Research, Helmholtz Centre for Infection Research, Inhoffenstraße. 7, 38124 Braunschweig, GermanyCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCellular Proteomics Research, Helmholtz Centre for Infection Research, Inhoffenstraße. 7, 38124 Braunschweig, GermanyCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalWe focus on the stalked goose barnacle <i>L. anatifera</i> adhesive system, an opportunistic less selective species for the substrate, found attached to a variety of floating objects at seas. Adhesion is an adaptative character in barnacles, ensuring adequate positioning in the habitat for feeding and reproduction. The protein composition of the cement multicomplex and adhesive gland was quantitatively studied using shotgun proteomic analysis. Overall, 11,795 peptide sequences were identified in the gland and 2206 in the cement, clustered in 1689 and 217 proteinGroups, respectively. Cement specific adhesive proteins (CPs), proteases, protease inhibitors, cuticular and structural proteins, chemical cues, and many unannotated proteins were found, among others. In the cement, CPs were the most abundant (80.5%), being the bulk proteins CP100k and -52k the most expressed of all, and CP43k-like the most expressed interfacial protein. Unannotated proteins comprised 4.7% of the cement proteome, ranking several of them among the most highly expressed. Eight of these proteins showed similar physicochemical properties and amino acid composition to known CPs and classified through Principal Components Analysis (PCA) as new CPs. The importance of PCA on the identification of unannotated non-conserved adhesive proteins, whose selective pressure is on their relative amino acid abundance, was demonstrated.https://www.mdpi.com/1422-0067/22/7/3370underwater adhesioncement proteinshotgun proteomicsiBAQprotein expressionmass spectrometry |
| spellingShingle | Dany Domínguez-Pérez Daniela Almeida Josef Wissing André M. Machado Lothar Jänsch Agostinho Antunes Luís Filipe Castro Vitor Vasconcelos Alexandre Campos Isabel Cunha Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle <i>Lepas anatifera</i> International Journal of Molecular Sciences underwater adhesion cement protein shotgun proteomics iBAQ protein expression mass spectrometry |
| title | Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle <i>Lepas anatifera</i> |
| title_full | Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle <i>Lepas anatifera</i> |
| title_fullStr | Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle <i>Lepas anatifera</i> |
| title_full_unstemmed | Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle <i>Lepas anatifera</i> |
| title_short | Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle <i>Lepas anatifera</i> |
| title_sort | proteogenomic characterization of the cement and adhesive gland of the pelagic gooseneck barnacle i lepas anatifera i |
| topic | underwater adhesion cement protein shotgun proteomics iBAQ protein expression mass spectrometry |
| url | https://www.mdpi.com/1422-0067/22/7/3370 |
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