Molecular characterization of buckwheat major immunoglobulin E-reactive proteins in allergic patients
Buckwheat extract was analyzed by immunoblotting experiments using sera from nine allergic and three non-allergic individuals. Major IgE-reactive bands were 73, 70, 62, 58 and 54 kDa under non-reducing conditions and were detected in allergic subjects, but not in non-allergic ones. Under reducing co...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2000-01-01
|
Series: | Allergology International |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893015313964 |
_version_ | 1811241476496031744 |
---|---|
author | Yuki Nagata Kaien Fujino Shuhei Hashiguchi Naoko Abe Yoshito Zaima Yuji Ito Yuriko Takahashi Keiji Maeda Kazuhisa Sugimura |
author_facet | Yuki Nagata Kaien Fujino Shuhei Hashiguchi Naoko Abe Yoshito Zaima Yuji Ito Yuriko Takahashi Keiji Maeda Kazuhisa Sugimura |
author_sort | Yuki Nagata |
collection | DOAJ |
description | Buckwheat extract was analyzed by immunoblotting experiments using sera from nine allergic and three non-allergic individuals. Major IgE-reactive bands were 73, 70, 62, 58 and 54 kDa under non-reducing conditions and were detected in allergic subjects, but not in non-allergic ones. Under reducing conditions, the 73, 70, 62 and 58 kDa bands split to 56 and 24, 52 and 24, 45 and 24, and 43 and 24 kDa, respectively. The 24 kDa molecule was the most prominent band recognized with IgE as well as IgG or IgA. The FA02 cDNA clone, encoding the α and β subunits of the legumin-like storage protein, was isolated from a cDNA library made of immature buckwheat seeds. The deduced amino acid sequence of the cDNA clone is substantially identical to the N-terminal amino acid sequence of the 24 kDa molecule, which may be identical to that of BW24KD reported by Urisu et al. Consistent with these results, the translation product of the cDNA encoding the putative β subunit was strongly recognized with serum IgE, IgG and IgA from buckwheat-allergic patients. These results suggested that the 24 kDa molecule may be the β subunit of the legumin-like storage molecule of buckwheat. |
first_indexed | 2024-04-12T13:36:44Z |
format | Article |
id | doaj.art-b841ef8db96d428e8713eafcb6629251 |
institution | Directory Open Access Journal |
issn | 1323-8930 |
language | English |
last_indexed | 2024-04-12T13:36:44Z |
publishDate | 2000-01-01 |
publisher | Elsevier |
record_format | Article |
series | Allergology International |
spelling | doaj.art-b841ef8db96d428e8713eafcb66292512022-12-22T03:30:58ZengElsevierAllergology International1323-89302000-01-0149211712410.1046/j.1440-1592.2000.00169.xMolecular characterization of buckwheat major immunoglobulin E-reactive proteins in allergic patientsYuki Nagata0Kaien Fujino1Shuhei Hashiguchi2Naoko Abe3Yoshito Zaima4Yuji Ito5Yuriko Takahashi6Keiji Maeda7Kazuhisa Sugimura8Department of Bioengineering, Faculty of Engineering, Kagoshima University, Kagoshima, JapanDepartment of Botany, Faculty of Agriculture, Hokkaido University, Sapporo, JapanDepartment of Bioengineering, Faculty of Engineering, Kagoshima University, Kagoshima, JapanDepartment of Bioengineering, Faculty of Engineering, Kagoshima University, Kagoshima, JapanDepartment of Bioengineering, Faculty of Engineering, Kagoshima University, Kagoshima, JapanDepartment of Bioengineering, Faculty of Engineering, Kagoshima University, Kagoshima, JapanDepartment of Pediatrics, Medical School of Yokohama City University, Yokohama, JapanDepartment of Internal Medicine, Osaka Teisin Hospital, Osaka, JapanDepartment of Bioengineering, Faculty of Engineering, Kagoshima University, Kagoshima, JapanBuckwheat extract was analyzed by immunoblotting experiments using sera from nine allergic and three non-allergic individuals. Major IgE-reactive bands were 73, 70, 62, 58 and 54 kDa under non-reducing conditions and were detected in allergic subjects, but not in non-allergic ones. Under reducing conditions, the 73, 70, 62 and 58 kDa bands split to 56 and 24, 52 and 24, 45 and 24, and 43 and 24 kDa, respectively. The 24 kDa molecule was the most prominent band recognized with IgE as well as IgG or IgA. The FA02 cDNA clone, encoding the α and β subunits of the legumin-like storage protein, was isolated from a cDNA library made of immature buckwheat seeds. The deduced amino acid sequence of the cDNA clone is substantially identical to the N-terminal amino acid sequence of the 24 kDa molecule, which may be identical to that of BW24KD reported by Urisu et al. Consistent with these results, the translation product of the cDNA encoding the putative β subunit was strongly recognized with serum IgE, IgG and IgA from buckwheat-allergic patients. These results suggested that the 24 kDa molecule may be the β subunit of the legumin-like storage molecule of buckwheat.http://www.sciencedirect.com/science/article/pii/S1323893015313964allergenbuckwheatcDNAimmunoblot analysislegumin |
spellingShingle | Yuki Nagata Kaien Fujino Shuhei Hashiguchi Naoko Abe Yoshito Zaima Yuji Ito Yuriko Takahashi Keiji Maeda Kazuhisa Sugimura Molecular characterization of buckwheat major immunoglobulin E-reactive proteins in allergic patients Allergology International allergen buckwheat cDNA immunoblot analysis legumin |
title | Molecular characterization of buckwheat major immunoglobulin E-reactive proteins in allergic patients |
title_full | Molecular characterization of buckwheat major immunoglobulin E-reactive proteins in allergic patients |
title_fullStr | Molecular characterization of buckwheat major immunoglobulin E-reactive proteins in allergic patients |
title_full_unstemmed | Molecular characterization of buckwheat major immunoglobulin E-reactive proteins in allergic patients |
title_short | Molecular characterization of buckwheat major immunoglobulin E-reactive proteins in allergic patients |
title_sort | molecular characterization of buckwheat major immunoglobulin e reactive proteins in allergic patients |
topic | allergen buckwheat cDNA immunoblot analysis legumin |
url | http://www.sciencedirect.com/science/article/pii/S1323893015313964 |
work_keys_str_mv | AT yukinagata molecularcharacterizationofbuckwheatmajorimmunoglobulinereactiveproteinsinallergicpatients AT kaienfujino molecularcharacterizationofbuckwheatmajorimmunoglobulinereactiveproteinsinallergicpatients AT shuheihashiguchi molecularcharacterizationofbuckwheatmajorimmunoglobulinereactiveproteinsinallergicpatients AT naokoabe molecularcharacterizationofbuckwheatmajorimmunoglobulinereactiveproteinsinallergicpatients AT yoshitozaima molecularcharacterizationofbuckwheatmajorimmunoglobulinereactiveproteinsinallergicpatients AT yujiito molecularcharacterizationofbuckwheatmajorimmunoglobulinereactiveproteinsinallergicpatients AT yurikotakahashi molecularcharacterizationofbuckwheatmajorimmunoglobulinereactiveproteinsinallergicpatients AT keijimaeda molecularcharacterizationofbuckwheatmajorimmunoglobulinereactiveproteinsinallergicpatients AT kazuhisasugimura molecularcharacterizationofbuckwheatmajorimmunoglobulinereactiveproteinsinallergicpatients |