Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line
OBJECTIVE: To assess intracellular labeling and quantification by magnetic resonance imaging using iron oxide magnetic nanoparticles coated with biocompatible materials in rat C6 glioma cells in vitro. These methods will provide direction for future trials of tumor induction in vivo as well as possi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Instituto Israelita de Ensino e Pesquisa Albert Einstein
2012-06-01
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| Series: | Einstein (São Paulo) |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200016&tlng=en |
| _version_ | 1819284080798203904 |
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| author | Javier Bustamante Mamani Lorena Favaro Pavon Liza Aya Mabuchi Miyaki Tatiana Tais Sibov Fabiana Rossan Paulo Henrique Silveira Walter Humberto Zavala Cárdenas Edson Amaro Junior Lionel Fernel Gamarra |
| author_facet | Javier Bustamante Mamani Lorena Favaro Pavon Liza Aya Mabuchi Miyaki Tatiana Tais Sibov Fabiana Rossan Paulo Henrique Silveira Walter Humberto Zavala Cárdenas Edson Amaro Junior Lionel Fernel Gamarra |
| author_sort | Javier Bustamante Mamani |
| collection | DOAJ |
| description | OBJECTIVE: To assess intracellular labeling and quantification by magnetic resonance imaging using iron oxide magnetic nanoparticles coated with biocompatible materials in rat C6 glioma cells in vitro. These methods will provide direction for future trials of tumor induction in vivo as well as possible magnetic hyperthermia applications. METHODS: Aminosilane, dextran, polyvinyl alcohol, and starch-coated magnetic nanoparticles were used in the qualitative assessment of C6 cell labeling via light microscopy. The influence of the transfection agent poly-L-lysine on cellular uptake was examined. The quantification process was performed by relaxometry analysis in T1 and T2weighted phantom images. RESULTS: Light microscopy revealed that the aminosilane-coated magnetic nanoparticles alone or complexed with poly-L-lysine showed higher cellular uptake than did the uncoated magnetic particles. The relaxivities of the aminosilane-coated magnetic nanoparticles with a hydrodynamic diameter of 50nm to a 3-T field were r1=(6.1±0.3)×10-5 ms-1mL/µg, r2=(5.3±0.1)× 10-4 ms-1mL/µg, with a ratio of r2 / r1 ≅ 9. The iron uptake in the cells was calculated by analyzing the relaxation rates (R1 and R2) using a mathematical relationship. CONCLUSIONS: C6 glioma cells have a high uptake efficiency for aminosilane-coated magnetic nanoparticles complexed with the transfection agent poly-L-lysine. The large ratio r2 / r1 ≅ 9 indicates that these magnetic nanoparticles are ideal for quantification by magnetic resonance imaging with T2-weighted imaging techniques. |
| first_indexed | 2024-12-24T01:41:42Z |
| format | Article |
| id | doaj.art-b842331120da438aadd65b739574c74e |
| institution | Directory Open Access Journal |
| issn | 2317-6385 |
| language | English |
| last_indexed | 2024-12-24T01:41:42Z |
| publishDate | 2012-06-01 |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-b842331120da438aadd65b739574c74e2022-12-21T17:21:59ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-63852012-06-01102216221Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell lineJavier Bustamante Mamani0Lorena Favaro Pavon1Liza Aya Mabuchi Miyaki2Tatiana Tais Sibov3Fabiana Rossan4Paulo Henrique Silveira5Walter Humberto Zavala Cárdenas6Edson Amaro Junior7Lionel Fernel Gamarra8Hospital Israelita Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinHospital Israelita Albert EinsteinOBJECTIVE: To assess intracellular labeling and quantification by magnetic resonance imaging using iron oxide magnetic nanoparticles coated with biocompatible materials in rat C6 glioma cells in vitro. These methods will provide direction for future trials of tumor induction in vivo as well as possible magnetic hyperthermia applications. METHODS: Aminosilane, dextran, polyvinyl alcohol, and starch-coated magnetic nanoparticles were used in the qualitative assessment of C6 cell labeling via light microscopy. The influence of the transfection agent poly-L-lysine on cellular uptake was examined. The quantification process was performed by relaxometry analysis in T1 and T2weighted phantom images. RESULTS: Light microscopy revealed that the aminosilane-coated magnetic nanoparticles alone or complexed with poly-L-lysine showed higher cellular uptake than did the uncoated magnetic particles. The relaxivities of the aminosilane-coated magnetic nanoparticles with a hydrodynamic diameter of 50nm to a 3-T field were r1=(6.1±0.3)×10-5 ms-1mL/µg, r2=(5.3±0.1)× 10-4 ms-1mL/µg, with a ratio of r2 / r1 ≅ 9. The iron uptake in the cells was calculated by analyzing the relaxation rates (R1 and R2) using a mathematical relationship. CONCLUSIONS: C6 glioma cells have a high uptake efficiency for aminosilane-coated magnetic nanoparticles complexed with the transfection agent poly-L-lysine. The large ratio r2 / r1 ≅ 9 indicates that these magnetic nanoparticles are ideal for quantification by magnetic resonance imaging with T2-weighted imaging techniques.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200016&tlng=enGliomaCell line, tumorNanoparticlesMagnetic resonance imaging |
| spellingShingle | Javier Bustamante Mamani Lorena Favaro Pavon Liza Aya Mabuchi Miyaki Tatiana Tais Sibov Fabiana Rossan Paulo Henrique Silveira Walter Humberto Zavala Cárdenas Edson Amaro Junior Lionel Fernel Gamarra Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line Einstein (São Paulo) Glioma Cell line, tumor Nanoparticles Magnetic resonance imaging |
| title | Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line |
| title_full | Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line |
| title_fullStr | Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line |
| title_full_unstemmed | Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line |
| title_short | Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line |
| title_sort | intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat c6 glioma cell line |
| topic | Glioma Cell line, tumor Nanoparticles Magnetic resonance imaging |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200016&tlng=en |
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