Effectiveness of various bioreactors for thraustochytrid culture and production (Aurantiochytruim limacinum BUCHAXM 122)
This study aimed to develop bioreactors for cultivation of thraustochytrid, Aurantiochytrium limacinum BUCHAXM 122, that are low in cost and simple to operate. Obtaining maximum biomass and fatty acid production was a prerequisite. Three bioreactor designs were used: stirred tank bioreactor (STB), b...
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PeerJ Inc.
2021-05-01
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| Online Access: | https://peerj.com/articles/11405.pdf |
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| author | Khanoksinee Sirirak Sorawit Powtongsook Sudarat Suanjit Somtawin Jaritkhuan |
| author_facet | Khanoksinee Sirirak Sorawit Powtongsook Sudarat Suanjit Somtawin Jaritkhuan |
| author_sort | Khanoksinee Sirirak |
| collection | DOAJ |
| description | This study aimed to develop bioreactors for cultivation of thraustochytrid, Aurantiochytrium limacinum BUCHAXM 122, that are low in cost and simple to operate. Obtaining maximum biomass and fatty acid production was a prerequisite. Three bioreactor designs were used: stirred tank bioreactor (STB), bubble bioreactor (BB) and internal loop airlift bioreactor (ILAB). The bioreactors were evaluated for their influence on oxygen mass transfer coefficient (kLa), using various spargers, mixing speed, and aeration rates. Biomass and DHA production from STB, BB, ILAB were then compared with an incubator shaker, using batch culture experiments. Results showed that a bundle of eight super-fine pore air stones was the best type of aeration sparger for all three bioreactors. Optimal culture conditions in STB were 600 rpm agitation speed and 2 vvm aeration rate, while 2 vvm and 1.5 vvm aeration provided highest biomass productivity in BB and ILAB, respectively. Antifoam agent was needed for all reactor types in order to reduce excessive foaming. Results indicated that with optimized conditions, these bioreactors are capable of thraustochytrid cultivation with a similar efficiency as cultivation using a rotary shaker. STB had the highest kLa and provided the highest biomass of 43.05 ± 0.35 g/L at 48 h. BB was simple in design, had low operating costs and was easy to build, but yielded the lowest biomass (27.50 ± 1.56 g/L). ILAB, on the other hand, had lower kLa than STB, but provided highest fatty acid productivity, of 35.36 ± 2.51% TFA. |
| first_indexed | 2024-03-09T06:38:32Z |
| format | Article |
| id | doaj.art-b8471d9cb5564f2f8992555101430fb3 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:38:32Z |
| publishDate | 2021-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-b8471d9cb5564f2f8992555101430fb32023-12-03T10:54:25ZengPeerJ Inc.PeerJ2167-83592021-05-019e1140510.7717/peerj.11405Effectiveness of various bioreactors for thraustochytrid culture and production (Aurantiochytruim limacinum BUCHAXM 122)Khanoksinee Sirirak0Sorawit Powtongsook1Sudarat Suanjit2Somtawin Jaritkhuan3Graduate Program in Environmental Science, Faculty of Science, Burapha University, Chon Buri, ThailandCenter of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, ThailandDepartment of Microbiology, Faculty of Science, Burapha University, Chon Buri, ThailandDepartment of Aquatic Science, Faculty of Science, Burapha University, Chon Buri, ThailandThis study aimed to develop bioreactors for cultivation of thraustochytrid, Aurantiochytrium limacinum BUCHAXM 122, that are low in cost and simple to operate. Obtaining maximum biomass and fatty acid production was a prerequisite. Three bioreactor designs were used: stirred tank bioreactor (STB), bubble bioreactor (BB) and internal loop airlift bioreactor (ILAB). The bioreactors were evaluated for their influence on oxygen mass transfer coefficient (kLa), using various spargers, mixing speed, and aeration rates. Biomass and DHA production from STB, BB, ILAB were then compared with an incubator shaker, using batch culture experiments. Results showed that a bundle of eight super-fine pore air stones was the best type of aeration sparger for all three bioreactors. Optimal culture conditions in STB were 600 rpm agitation speed and 2 vvm aeration rate, while 2 vvm and 1.5 vvm aeration provided highest biomass productivity in BB and ILAB, respectively. Antifoam agent was needed for all reactor types in order to reduce excessive foaming. Results indicated that with optimized conditions, these bioreactors are capable of thraustochytrid cultivation with a similar efficiency as cultivation using a rotary shaker. STB had the highest kLa and provided the highest biomass of 43.05 ± 0.35 g/L at 48 h. BB was simple in design, had low operating costs and was easy to build, but yielded the lowest biomass (27.50 ± 1.56 g/L). ILAB, on the other hand, had lower kLa than STB, but provided highest fatty acid productivity, of 35.36 ± 2.51% TFA.https://peerj.com/articles/11405.pdfAurantiochytrium limacinumOxygen mass transfer coefficientStirred tank bioreactorBubble bioreactorInternal loop airlift bioreactorDocosahexaenoic acid |
| spellingShingle | Khanoksinee Sirirak Sorawit Powtongsook Sudarat Suanjit Somtawin Jaritkhuan Effectiveness of various bioreactors for thraustochytrid culture and production (Aurantiochytruim limacinum BUCHAXM 122) PeerJ Aurantiochytrium limacinum Oxygen mass transfer coefficient Stirred tank bioreactor Bubble bioreactor Internal loop airlift bioreactor Docosahexaenoic acid |
| title | Effectiveness of various bioreactors for thraustochytrid culture and production (Aurantiochytruim limacinum BUCHAXM 122) |
| title_full | Effectiveness of various bioreactors for thraustochytrid culture and production (Aurantiochytruim limacinum BUCHAXM 122) |
| title_fullStr | Effectiveness of various bioreactors for thraustochytrid culture and production (Aurantiochytruim limacinum BUCHAXM 122) |
| title_full_unstemmed | Effectiveness of various bioreactors for thraustochytrid culture and production (Aurantiochytruim limacinum BUCHAXM 122) |
| title_short | Effectiveness of various bioreactors for thraustochytrid culture and production (Aurantiochytruim limacinum BUCHAXM 122) |
| title_sort | effectiveness of various bioreactors for thraustochytrid culture and production aurantiochytruim limacinum buchaxm 122 |
| topic | Aurantiochytrium limacinum Oxygen mass transfer coefficient Stirred tank bioreactor Bubble bioreactor Internal loop airlift bioreactor Docosahexaenoic acid |
| url | https://peerj.com/articles/11405.pdf |
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