A Design of Experiment Approach to Optimize Spray-Dried Powders Containing <i>Pseudomonas aeruginosa</i><i>Podoviridae</i> and <i>Myoviridae</i> Bacteriophages
In the present study, we evaluated the effect of spray-drying formulations and operating parameters of a laboratory-scale spray-dryer on the characteristics of spray-dried powders containing two <i>Pseudomonas aeruginosa</i> bacteriophages exhibiting different morphotypes: a podovirus (L...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/10/1926 |
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| author | Emilie Tabare Tea Glonti Christel Cochez Cyrille Ngassam Jean-Paul Pirnay Karim Amighi Jonathan Goole |
| author_facet | Emilie Tabare Tea Glonti Christel Cochez Cyrille Ngassam Jean-Paul Pirnay Karim Amighi Jonathan Goole |
| author_sort | Emilie Tabare |
| collection | DOAJ |
| description | In the present study, we evaluated the effect of spray-drying formulations and operating parameters of a laboratory-scale spray-dryer on the characteristics of spray-dried powders containing two <i>Pseudomonas aeruginosa</i> bacteriophages exhibiting different morphotypes: a podovirus (LUZ19) and a myovirus (14-1). We optimized the production process for bacteriophage-loaded powders, with an emphasis on long-term storage under ICH (international conference on harmonization) conditions. D-trehalose-/L-isoleucine-containing bacteriophage mixtures were spray-dried from aqueous solutions using a Büchi Mini Spray-dryer B-290 (Flawil, Switzerland). A response surface methodology was used for the optimization of the spray-drying process, with the following as-evaluated parameters: Inlet temperature, spray gas flow rate, and the D-trehalose/L-isoleucine ratio. The dried powders were characterized in terms of yield, residual moisture content, and bacteriophage lytic activity. L-isoleucine has demonstrated a positive impact on the activity of LUZ19, but a negative impact on 14-1. We observed a negligible impact of the inlet temperature and a positive correlation of the spray gas flow rate with bacteriophage activity. After optimization, we were able to obtain dry powder preparations of both bacteriophages, which were stable for a minimum of one year under different ICH storage conditions (up to and including 40 °C and 75% relative humidity). |
| first_indexed | 2024-03-10T06:08:47Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T06:08:47Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-b84d02b5db8d42bd85cdaf0894de8d932023-11-22T20:18:09ZengMDPI AGViruses1999-49152021-09-011310192610.3390/v13101926A Design of Experiment Approach to Optimize Spray-Dried Powders Containing <i>Pseudomonas aeruginosa</i><i>Podoviridae</i> and <i>Myoviridae</i> BacteriophagesEmilie Tabare0Tea Glonti1Christel Cochez2Cyrille Ngassam3Jean-Paul Pirnay4Karim Amighi5Jonathan Goole6Laboratory of Pharmaceutics and Biopharmaceutics, Faculty of Pharmacy, ULB, 1050 Brussels, BelgiumLaboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, 1120 Brussels, BelgiumLaboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, 1120 Brussels, BelgiumVesale Pharma S.A., 5310 Noville-sur-Mehaigne, BelgiumLaboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, 1120 Brussels, BelgiumLaboratory of Pharmaceutics and Biopharmaceutics, Faculty of Pharmacy, ULB, 1050 Brussels, BelgiumLaboratory of Pharmaceutics and Biopharmaceutics, Faculty of Pharmacy, ULB, 1050 Brussels, BelgiumIn the present study, we evaluated the effect of spray-drying formulations and operating parameters of a laboratory-scale spray-dryer on the characteristics of spray-dried powders containing two <i>Pseudomonas aeruginosa</i> bacteriophages exhibiting different morphotypes: a podovirus (LUZ19) and a myovirus (14-1). We optimized the production process for bacteriophage-loaded powders, with an emphasis on long-term storage under ICH (international conference on harmonization) conditions. D-trehalose-/L-isoleucine-containing bacteriophage mixtures were spray-dried from aqueous solutions using a Büchi Mini Spray-dryer B-290 (Flawil, Switzerland). A response surface methodology was used for the optimization of the spray-drying process, with the following as-evaluated parameters: Inlet temperature, spray gas flow rate, and the D-trehalose/L-isoleucine ratio. The dried powders were characterized in terms of yield, residual moisture content, and bacteriophage lytic activity. L-isoleucine has demonstrated a positive impact on the activity of LUZ19, but a negative impact on 14-1. We observed a negligible impact of the inlet temperature and a positive correlation of the spray gas flow rate with bacteriophage activity. After optimization, we were able to obtain dry powder preparations of both bacteriophages, which were stable for a minimum of one year under different ICH storage conditions (up to and including 40 °C and 75% relative humidity).https://www.mdpi.com/1999-4915/13/10/1926DoEQbDantibiotic-resistantbacteriophage therapyspray-dryingdrying |
| spellingShingle | Emilie Tabare Tea Glonti Christel Cochez Cyrille Ngassam Jean-Paul Pirnay Karim Amighi Jonathan Goole A Design of Experiment Approach to Optimize Spray-Dried Powders Containing <i>Pseudomonas aeruginosa</i><i>Podoviridae</i> and <i>Myoviridae</i> Bacteriophages Viruses DoE QbD antibiotic-resistant bacteriophage therapy spray-drying drying |
| title | A Design of Experiment Approach to Optimize Spray-Dried Powders Containing <i>Pseudomonas aeruginosa</i><i>Podoviridae</i> and <i>Myoviridae</i> Bacteriophages |
| title_full | A Design of Experiment Approach to Optimize Spray-Dried Powders Containing <i>Pseudomonas aeruginosa</i><i>Podoviridae</i> and <i>Myoviridae</i> Bacteriophages |
| title_fullStr | A Design of Experiment Approach to Optimize Spray-Dried Powders Containing <i>Pseudomonas aeruginosa</i><i>Podoviridae</i> and <i>Myoviridae</i> Bacteriophages |
| title_full_unstemmed | A Design of Experiment Approach to Optimize Spray-Dried Powders Containing <i>Pseudomonas aeruginosa</i><i>Podoviridae</i> and <i>Myoviridae</i> Bacteriophages |
| title_short | A Design of Experiment Approach to Optimize Spray-Dried Powders Containing <i>Pseudomonas aeruginosa</i><i>Podoviridae</i> and <i>Myoviridae</i> Bacteriophages |
| title_sort | design of experiment approach to optimize spray dried powders containing i pseudomonas aeruginosa i i podoviridae i and i myoviridae i bacteriophages |
| topic | DoE QbD antibiotic-resistant bacteriophage therapy spray-drying drying |
| url | https://www.mdpi.com/1999-4915/13/10/1926 |
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