Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma
Long-chain non-coding RNA (LncRNA) has been found to play an important role in the regulation of the occurrence and progression of renal cell carcinoma (RCC). In this study, we demonstrated that LncRNA NEAT1 expression and m6A methylation level was decreased in RCC tissues. Further, the downregulate...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-12-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.777349/full |
| _version_ | 1818435478650617856 |
|---|---|
| author | Jieqing Chen Jieqing Chen Jieqing Chen Jieqing Chen Xinhui Liao Xinhui Liao Xinhui Liao Xinhui Liao Jianli Cheng Jianli Cheng Jianli Cheng Ganglin Su Ganglin Su Ganglin Su Fen Yuan Fen Yuan Fen Yuan Zhongfu Zhang Zhongfu Zhang Zhongfu Zhang Jianting Wu Jianting Wu Jianting Wu Hongbing Mei Hongbing Mei Hongbing Mei Wanlong Tan |
| author_facet | Jieqing Chen Jieqing Chen Jieqing Chen Jieqing Chen Xinhui Liao Xinhui Liao Xinhui Liao Xinhui Liao Jianli Cheng Jianli Cheng Jianli Cheng Ganglin Su Ganglin Su Ganglin Su Fen Yuan Fen Yuan Fen Yuan Zhongfu Zhang Zhongfu Zhang Zhongfu Zhang Jianting Wu Jianting Wu Jianting Wu Hongbing Mei Hongbing Mei Hongbing Mei Wanlong Tan |
| author_sort | Jieqing Chen |
| collection | DOAJ |
| description | Long-chain non-coding RNA (LncRNA) has been found to play an important role in the regulation of the occurrence and progression of renal cell carcinoma (RCC). In this study, we demonstrated that LncRNA NEAT1 expression and m6A methylation level was decreased in RCC tissues. Further, the downregulated expression level of LncRNA NEAT1 was associated with poor prognosis for RCC patients. Then we used CRIPSR/dCas13b-METTL3 to methylate LncRNA NEAT1 in RCC cells. The results showed that the expression level of LncRNA NEAT1 was upregulated after methylated by dCas13b-METTL3 in RCC cells. And the proliferation and migration ability of RCC cells was decreased after methylated LncRNA NEAT1. Finally, we examined the effect of LncRNA NEAT1 hypermethylation on the transcriptome. We found differentially expressed genes in RCC cells were associated with “cGMP-PKG signaling pathway”, “Cell adhesion molecules” and “Pathways in cancer”. In conclusion, CRISPR/Cas13b-METTL3 targeting LncRNA NEAT1 m6A methylation activates LncRNA NEAT1 expression and provides a new target for treatment of RCC. |
| first_indexed | 2024-12-14T16:53:32Z |
| format | Article |
| id | doaj.art-b84d2003232d4da5a9d041e71374a887 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-14T16:53:32Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-b84d2003232d4da5a9d041e71374a8872022-12-21T22:54:01ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-12-01910.3389/fcell.2021.777349777349Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell CarcinomaJieqing Chen0Jieqing Chen1Jieqing Chen2Jieqing Chen3Xinhui Liao4Xinhui Liao5Xinhui Liao6Xinhui Liao7Jianli Cheng8Jianli Cheng9Jianli Cheng10Ganglin Su11Ganglin Su12Ganglin Su13Fen Yuan14Fen Yuan15Fen Yuan16Zhongfu Zhang17Zhongfu Zhang18Zhongfu Zhang19Jianting Wu20Jianting Wu21Jianting Wu22Hongbing Mei23Hongbing Mei24Hongbing Mei25Wanlong Tan26Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaDepartment of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaDepartment of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaDepartment of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaDepartment of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaDepartment of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaDepartment of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaDepartment of Urology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaGuangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Genitourinary Tumor, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaDepartment of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaLong-chain non-coding RNA (LncRNA) has been found to play an important role in the regulation of the occurrence and progression of renal cell carcinoma (RCC). In this study, we demonstrated that LncRNA NEAT1 expression and m6A methylation level was decreased in RCC tissues. Further, the downregulated expression level of LncRNA NEAT1 was associated with poor prognosis for RCC patients. Then we used CRIPSR/dCas13b-METTL3 to methylate LncRNA NEAT1 in RCC cells. The results showed that the expression level of LncRNA NEAT1 was upregulated after methylated by dCas13b-METTL3 in RCC cells. And the proliferation and migration ability of RCC cells was decreased after methylated LncRNA NEAT1. Finally, we examined the effect of LncRNA NEAT1 hypermethylation on the transcriptome. We found differentially expressed genes in RCC cells were associated with “cGMP-PKG signaling pathway”, “Cell adhesion molecules” and “Pathways in cancer”. In conclusion, CRISPR/Cas13b-METTL3 targeting LncRNA NEAT1 m6A methylation activates LncRNA NEAT1 expression and provides a new target for treatment of RCC.https://www.frontiersin.org/articles/10.3389/fcell.2021.777349/fulllncRNANEAT1m6A (N6-methyladenosine)RCC = renal cell cancerdCas13b |
| spellingShingle | Jieqing Chen Jieqing Chen Jieqing Chen Jieqing Chen Xinhui Liao Xinhui Liao Xinhui Liao Xinhui Liao Jianli Cheng Jianli Cheng Jianli Cheng Ganglin Su Ganglin Su Ganglin Su Fen Yuan Fen Yuan Fen Yuan Zhongfu Zhang Zhongfu Zhang Zhongfu Zhang Jianting Wu Jianting Wu Jianting Wu Hongbing Mei Hongbing Mei Hongbing Mei Wanlong Tan Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma Frontiers in Cell and Developmental Biology lncRNA NEAT1 m6A (N6-methyladenosine) RCC = renal cell cancer dCas13b |
| title | Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
| title_full | Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
| title_fullStr | Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
| title_full_unstemmed | Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
| title_short | Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma |
| title_sort | targeted methylation of the lncrna neat1 suppresses malignancy of renal cell carcinoma |
| topic | lncRNA NEAT1 m6A (N6-methyladenosine) RCC = renal cell cancer dCas13b |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.777349/full |
| work_keys_str_mv | AT jieqingchen targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT jieqingchen targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT jieqingchen targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT jieqingchen targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT xinhuiliao targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT xinhuiliao targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT xinhuiliao targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT xinhuiliao targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT jianlicheng targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT jianlicheng targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT jianlicheng targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT ganglinsu targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT ganglinsu targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT ganglinsu targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT fenyuan targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT fenyuan targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT fenyuan targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT zhongfuzhang targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT zhongfuzhang targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT zhongfuzhang targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT jiantingwu targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT jiantingwu targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT jiantingwu targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT hongbingmei targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT hongbingmei targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT hongbingmei targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma AT wanlongtan targetedmethylationofthelncrnaneat1suppressesmalignancyofrenalcellcarcinoma |