Comparison of In-vitro Activities of Linezolid and Vancomycin against Staphylococcus aureus Isolated from A Tertiary Care Hospital
Background: Methicillin resistant Staphylococcus aureus (MRSA) has emerged as one of the commonest cause of hospital acquired infections worldwide. Vancomycin is the antibiotic of choice for treatment of MRSA, but due to slow increase in vancomycin minimum inhibitory concentration (MIC) (vancomy...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2014-05-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/4338/7751_CE(Ra)_E(C)_F(H)_PF1(AGH)_PFA(P)_PF2(PAG).pdf |
| _version_ | 1818316953423446016 |
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| author | Sachin Kishore Deepika Verma ME Siddique |
| author_facet | Sachin Kishore Deepika Verma ME Siddique |
| author_sort | Sachin Kishore |
| collection | DOAJ |
| description | Background: Methicillin resistant Staphylococcus aureus
(MRSA) has emerged as one of the commonest cause of hospital
acquired infections worldwide. Vancomycin is the antibiotic
of choice for treatment of MRSA, but due to slow increase in
vancomycin minimum inhibitory concentration (MIC) (vancomycin
creep),Vancomycin has become a suboptimal therapeutic option
in critically ill patients. Linezolid has emerged as an alternative
drug in the treatment of such cases.
Aim: To compare in vitro activities of linezolid and vancomycin
against Staphylococcus aureus, in order to help in formulating a
better treatment.
Method: 200 strains of Staphylococcus aureus were isolated
from different clinical specimens between April 2010 to March
2011. Antibiotic sensitivity testing was performed by Kirby Bauer
disc diffusion method and MICs of vancomycin and linezolid
were determined for all 200 strains by agar dilution method by
following CLSI guidelines.
Results: Among 200 strains, MIC for linezolid was 4 µg/ml for
3 strains, MIC was 2 µg/ml for 71 strains, and MIC was 1 µg/ml
for 126 strains, while for the same 200 strains of Staphylococcus
aureus, MIC of vancomycin was 4 µg/ml for 8 strains, it was 2 µg/
ml for 103 strains and it was 1 µg/ml for 89 strains.
Conclusion: Linezolid and vancomycin had similar in-vitro
efficacies for Staphylococcus aureus in disc diffusion method, but
the number of strains with higher ranges of MICs of vancomycin
(1-4 µg/ml) were more as compared to those which had higher
ranges of MICs for linezolid. So, we suggest that linezolid can
be a good alternative for the treatment of multidrug resistant
Staphylococcus aureus as compared to vancomycin. |
| first_indexed | 2024-12-13T09:29:37Z |
| format | Article |
| id | doaj.art-b84f61d91a4c4e94a63d0084c6d4c6f5 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-13T09:29:37Z |
| publishDate | 2014-05-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-b84f61d91a4c4e94a63d0084c6d4c6f52022-12-21T23:52:32ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-05-0185DC12DC1510.7860/JCDR/2014/7751.4338Comparison of In-vitro Activities of Linezolid and Vancomycin against Staphylococcus aureus Isolated from A Tertiary Care HospitalSachin Kishore0Deepika Verma1ME Siddique2Assistant Professor, Department of Microbiology, Rohilkhand Medical College and Hospital, Pilibhit Bypass, Bareilly-243006, UP, India.Assistant Professor, Department of Microbiology, Rohilkhand Medical College and Hospital, Pilibhit Bypass, Bareilly-243006, UP, India.Professor, Department of Microbiology, Rohilkhand Medical College and Hospital, Pilibhit Bypass, Bareilly-243006, UP, India.Background: Methicillin resistant Staphylococcus aureus (MRSA) has emerged as one of the commonest cause of hospital acquired infections worldwide. Vancomycin is the antibiotic of choice for treatment of MRSA, but due to slow increase in vancomycin minimum inhibitory concentration (MIC) (vancomycin creep),Vancomycin has become a suboptimal therapeutic option in critically ill patients. Linezolid has emerged as an alternative drug in the treatment of such cases. Aim: To compare in vitro activities of linezolid and vancomycin against Staphylococcus aureus, in order to help in formulating a better treatment. Method: 200 strains of Staphylococcus aureus were isolated from different clinical specimens between April 2010 to March 2011. Antibiotic sensitivity testing was performed by Kirby Bauer disc diffusion method and MICs of vancomycin and linezolid were determined for all 200 strains by agar dilution method by following CLSI guidelines. Results: Among 200 strains, MIC for linezolid was 4 µg/ml for 3 strains, MIC was 2 µg/ml for 71 strains, and MIC was 1 µg/ml for 126 strains, while for the same 200 strains of Staphylococcus aureus, MIC of vancomycin was 4 µg/ml for 8 strains, it was 2 µg/ ml for 103 strains and it was 1 µg/ml for 89 strains. Conclusion: Linezolid and vancomycin had similar in-vitro efficacies for Staphylococcus aureus in disc diffusion method, but the number of strains with higher ranges of MICs of vancomycin (1-4 µg/ml) were more as compared to those which had higher ranges of MICs for linezolid. So, we suggest that linezolid can be a good alternative for the treatment of multidrug resistant Staphylococcus aureus as compared to vancomycin.https://jcdr.net/articles/PDF/4338/7751_CE(Ra)_E(C)_F(H)_PF1(AGH)_PFA(P)_PF2(PAG).pdfvisamrsamic |
| spellingShingle | Sachin Kishore Deepika Verma ME Siddique Comparison of In-vitro Activities of Linezolid and Vancomycin against Staphylococcus aureus Isolated from A Tertiary Care Hospital Journal of Clinical and Diagnostic Research visa mrsa mic |
| title | Comparison of In-vitro Activities of Linezolid and Vancomycin against Staphylococcus aureus Isolated from A Tertiary Care Hospital |
| title_full | Comparison of In-vitro Activities of Linezolid and Vancomycin against Staphylococcus aureus Isolated from A Tertiary Care Hospital |
| title_fullStr | Comparison of In-vitro Activities of Linezolid and Vancomycin against Staphylococcus aureus Isolated from A Tertiary Care Hospital |
| title_full_unstemmed | Comparison of In-vitro Activities of Linezolid and Vancomycin against Staphylococcus aureus Isolated from A Tertiary Care Hospital |
| title_short | Comparison of In-vitro Activities of Linezolid and Vancomycin against Staphylococcus aureus Isolated from A Tertiary Care Hospital |
| title_sort | comparison of in vitro activities of linezolid and vancomycin against staphylococcus aureus isolated from a tertiary care hospital |
| topic | visa mrsa mic |
| url | https://jcdr.net/articles/PDF/4338/7751_CE(Ra)_E(C)_F(H)_PF1(AGH)_PFA(P)_PF2(PAG).pdf |
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