Liver Proteome Alterations in Red Deer (<i>Cervus elaphus</i>) Infected by the Giant Liver Fluke <i>Fascioloides magna</i>
Liver fluke infections are recognised as diseases with worldwide distribution and considerable veterinary and public health importance. The giant liver fluke, <i>Fascioloides magna</i>, is an important non-native parasite which has been introduced to Europe, posing a threat to the surviv...
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MDPI AG
2022-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/12/1503 |
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| author | Karol Šimonji Dean Konjević Miljenko Bujanić Ivana Rubić Vladimir Farkaš Anđelo Beletić Lea Grbavac Josipa Kuleš |
| author_facet | Karol Šimonji Dean Konjević Miljenko Bujanić Ivana Rubić Vladimir Farkaš Anđelo Beletić Lea Grbavac Josipa Kuleš |
| author_sort | Karol Šimonji |
| collection | DOAJ |
| description | Liver fluke infections are recognised as diseases with worldwide distribution and considerable veterinary and public health importance. The giant liver fluke, <i>Fascioloides magna</i>, is an important non-native parasite which has been introduced to Europe, posing a threat to the survival of local wildlife populations such as red deer (<i>Cervus elaphus</i>). The aim of the study was to analyse differences in liver proteomes between <i>F. magna</i>-infected and control red deer groups using a label-based high-throughput quantitative proteomics approach. The proteomics analysis identified 234 proteins with differential abundance between the control and infected groups. Our findings showed that <i>F. magna</i> infection in this definitive host is associated with changes in the metabolism of proteins and fatty acids, oxidative stress, fibrosis, and signaling pathways. The identified proteins and associated biological pathways represent a valuable contribution to the understanding of host–parasite interactions and the pathogenesis of liver fluke infection. |
| first_indexed | 2024-03-09T15:59:39Z |
| format | Article |
| id | doaj.art-b8542137fa6448c897454f733376b406 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T15:59:39Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-b8542137fa6448c897454f733376b4062023-11-24T17:13:49ZengMDPI AGPathogens2076-08172022-12-011112150310.3390/pathogens11121503Liver Proteome Alterations in Red Deer (<i>Cervus elaphus</i>) Infected by the Giant Liver Fluke <i>Fascioloides magna</i>Karol Šimonji0Dean Konjević1Miljenko Bujanić2Ivana Rubić3Vladimir Farkaš4Anđelo Beletić5Lea Grbavac6Josipa Kuleš7Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, CroatiaDepartment of Veterinary Economics and Epidemiology, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, CroatiaDepartment of Veterinary Economics and Epidemiology, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, CroatiaLaboratory of Proteomics, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, CroatiaLaboratory of Proteomics, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, CroatiaLaboratory of Proteomics, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, CroatiaDepartment of Parasitology and Parasitic Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, CroatiaDepartment of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, CroatiaLiver fluke infections are recognised as diseases with worldwide distribution and considerable veterinary and public health importance. The giant liver fluke, <i>Fascioloides magna</i>, is an important non-native parasite which has been introduced to Europe, posing a threat to the survival of local wildlife populations such as red deer (<i>Cervus elaphus</i>). The aim of the study was to analyse differences in liver proteomes between <i>F. magna</i>-infected and control red deer groups using a label-based high-throughput quantitative proteomics approach. The proteomics analysis identified 234 proteins with differential abundance between the control and infected groups. Our findings showed that <i>F. magna</i> infection in this definitive host is associated with changes in the metabolism of proteins and fatty acids, oxidative stress, fibrosis, and signaling pathways. The identified proteins and associated biological pathways represent a valuable contribution to the understanding of host–parasite interactions and the pathogenesis of liver fluke infection.https://www.mdpi.com/2076-0817/11/12/1503wildlifeliver flukehost-pathogen interactionproteomicstandem mass tag |
| spellingShingle | Karol Šimonji Dean Konjević Miljenko Bujanić Ivana Rubić Vladimir Farkaš Anđelo Beletić Lea Grbavac Josipa Kuleš Liver Proteome Alterations in Red Deer (<i>Cervus elaphus</i>) Infected by the Giant Liver Fluke <i>Fascioloides magna</i> Pathogens wildlife liver fluke host-pathogen interaction proteomics tandem mass tag |
| title | Liver Proteome Alterations in Red Deer (<i>Cervus elaphus</i>) Infected by the Giant Liver Fluke <i>Fascioloides magna</i> |
| title_full | Liver Proteome Alterations in Red Deer (<i>Cervus elaphus</i>) Infected by the Giant Liver Fluke <i>Fascioloides magna</i> |
| title_fullStr | Liver Proteome Alterations in Red Deer (<i>Cervus elaphus</i>) Infected by the Giant Liver Fluke <i>Fascioloides magna</i> |
| title_full_unstemmed | Liver Proteome Alterations in Red Deer (<i>Cervus elaphus</i>) Infected by the Giant Liver Fluke <i>Fascioloides magna</i> |
| title_short | Liver Proteome Alterations in Red Deer (<i>Cervus elaphus</i>) Infected by the Giant Liver Fluke <i>Fascioloides magna</i> |
| title_sort | liver proteome alterations in red deer i cervus elaphus i infected by the giant liver fluke i fascioloides magna i |
| topic | wildlife liver fluke host-pathogen interaction proteomics tandem mass tag |
| url | https://www.mdpi.com/2076-0817/11/12/1503 |
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