Expression Levels of Heat-Shock Proteins in <i>Apis mellifera jemenetica</i> and <i>Apis mellifera carnica</i> Foragers in the Desert Climate of Saudi Arabia
<i>A. m. jemenetica</i> is the indigenous honeybee of the Arabian Peninsula. It is highly adapted to extreme temperatures exceeding 40 °C, yet important molecular aspects of its adaptation are not well documented. In this study we quantify relative expression levels of small- and large-m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/5/432 |
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| author | Ahmad A. Alghamdi Yehya Z. Alattal |
| author_facet | Ahmad A. Alghamdi Yehya Z. Alattal |
| author_sort | Ahmad A. Alghamdi |
| collection | DOAJ |
| description | <i>A. m. jemenetica</i> is the indigenous honeybee of the Arabian Peninsula. It is highly adapted to extreme temperatures exceeding 40 °C, yet important molecular aspects of its adaptation are not well documented. In this study we quantify relative expression levels of small- and large-molecular-weight heat-shock proteins (<i>hsp10</i>, <i>hsp28</i>, <i>hsp70</i>, <i>hsp83</i>, <i>hsp90</i> and <i>hsc70</i> (mRNAs)) in the thermos-tolerant <i>A. m. jemenetica</i> and thermosusceptible <i>A. m. carnica</i> forager honeybee subspecies under desert (Riyadh) and semi-arid (Baha) summer conditions. The results showed significant day-long higher expression levels of <i>hsp</i> mRNAs in <i>A. m. jemenetica</i> compared to <i>A. m. carnica</i> under the same conditions. In Baha, the expression levels were very modest in both subspecies compared those in Riyadh though the expression levels were higher <i>in A. m. jemenetica</i>. The results also revealed a significant interaction between subspecies, which indicated milder stress conditions in Baha. In conclusion, the higher expression levels of <i>hsp10</i>, <i>hsp28</i>, <i>hsp70ab</i>, <i>hsp83</i> and <i>hsp90</i> mRNAs in <i>A. m. jemenetica</i> are key elements in the adaptive nature of <i>A. m. jemenetica</i> to local conditions that enhance its survival and fitness in high summer temperatures. |
| first_indexed | 2024-03-11T03:38:53Z |
| format | Article |
| id | doaj.art-b855c94b4b4b4230a8ecffc7c6c33db8 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T03:38:53Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-b855c94b4b4b4230a8ecffc7c6c33db82023-11-18T01:49:49ZengMDPI AGInsects2075-44502023-04-0114543210.3390/insects14050432Expression Levels of Heat-Shock Proteins in <i>Apis mellifera jemenetica</i> and <i>Apis mellifera carnica</i> Foragers in the Desert Climate of Saudi ArabiaAhmad A. Alghamdi0Yehya Z. Alattal1Department of Plant Protection, Chair of Engineer Abdullah Ahmad Bagshan for Bee Research, College of Food and Agriculture Sciences, King Saud University, Riyadh 11587, Saudi ArabiaDepartment of Plant Protection, Chair of Engineer Abdullah Ahmad Bagshan for Bee Research, College of Food and Agriculture Sciences, King Saud University, Riyadh 11587, Saudi Arabia<i>A. m. jemenetica</i> is the indigenous honeybee of the Arabian Peninsula. It is highly adapted to extreme temperatures exceeding 40 °C, yet important molecular aspects of its adaptation are not well documented. In this study we quantify relative expression levels of small- and large-molecular-weight heat-shock proteins (<i>hsp10</i>, <i>hsp28</i>, <i>hsp70</i>, <i>hsp83</i>, <i>hsp90</i> and <i>hsc70</i> (mRNAs)) in the thermos-tolerant <i>A. m. jemenetica</i> and thermosusceptible <i>A. m. carnica</i> forager honeybee subspecies under desert (Riyadh) and semi-arid (Baha) summer conditions. The results showed significant day-long higher expression levels of <i>hsp</i> mRNAs in <i>A. m. jemenetica</i> compared to <i>A. m. carnica</i> under the same conditions. In Baha, the expression levels were very modest in both subspecies compared those in Riyadh though the expression levels were higher <i>in A. m. jemenetica</i>. The results also revealed a significant interaction between subspecies, which indicated milder stress conditions in Baha. In conclusion, the higher expression levels of <i>hsp10</i>, <i>hsp28</i>, <i>hsp70ab</i>, <i>hsp83</i> and <i>hsp90</i> mRNAs in <i>A. m. jemenetica</i> are key elements in the adaptive nature of <i>A. m. jemenetica</i> to local conditions that enhance its survival and fitness in high summer temperatures.https://www.mdpi.com/2075-4450/14/5/432heat-shock proteinthermo-toleranceqPCR<i>hsp70</i><i>hsp28</i><i>hsc70</i> |
| spellingShingle | Ahmad A. Alghamdi Yehya Z. Alattal Expression Levels of Heat-Shock Proteins in <i>Apis mellifera jemenetica</i> and <i>Apis mellifera carnica</i> Foragers in the Desert Climate of Saudi Arabia Insects heat-shock protein thermo-tolerance qPCR <i>hsp70</i> <i>hsp28</i> <i>hsc70</i> |
| title | Expression Levels of Heat-Shock Proteins in <i>Apis mellifera jemenetica</i> and <i>Apis mellifera carnica</i> Foragers in the Desert Climate of Saudi Arabia |
| title_full | Expression Levels of Heat-Shock Proteins in <i>Apis mellifera jemenetica</i> and <i>Apis mellifera carnica</i> Foragers in the Desert Climate of Saudi Arabia |
| title_fullStr | Expression Levels of Heat-Shock Proteins in <i>Apis mellifera jemenetica</i> and <i>Apis mellifera carnica</i> Foragers in the Desert Climate of Saudi Arabia |
| title_full_unstemmed | Expression Levels of Heat-Shock Proteins in <i>Apis mellifera jemenetica</i> and <i>Apis mellifera carnica</i> Foragers in the Desert Climate of Saudi Arabia |
| title_short | Expression Levels of Heat-Shock Proteins in <i>Apis mellifera jemenetica</i> and <i>Apis mellifera carnica</i> Foragers in the Desert Climate of Saudi Arabia |
| title_sort | expression levels of heat shock proteins in i apis mellifera jemenetica i and i apis mellifera carnica i foragers in the desert climate of saudi arabia |
| topic | heat-shock protein thermo-tolerance qPCR <i>hsp70</i> <i>hsp28</i> <i>hsc70</i> |
| url | https://www.mdpi.com/2075-4450/14/5/432 |
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