Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets
Abstract Background Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in humans, cows, and pigs. The gut microbiota underlies pathology of several infectious diseases yet the role of the gut microbiota in the pathogenesis of ETEC-induced diarrhea is unknown. Results By using an ETEC induced di...
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1704-9 |
| _version_ | 1819330860804997120 |
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| author | Peng Bin Zhiyi Tang Shaojuan Liu Shuai Chen Yaoyao Xia Jiaqi Liu Hucong Wu Guoqiang Zhu |
| author_facet | Peng Bin Zhiyi Tang Shaojuan Liu Shuai Chen Yaoyao Xia Jiaqi Liu Hucong Wu Guoqiang Zhu |
| author_sort | Peng Bin |
| collection | DOAJ |
| description | Abstract Background Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in humans, cows, and pigs. The gut microbiota underlies pathology of several infectious diseases yet the role of the gut microbiota in the pathogenesis of ETEC-induced diarrhea is unknown. Results By using an ETEC induced diarrheal model in piglet, we profiled the jejunal and fecal microbiota using metagenomics and 16S rRNA sequencing. A jejunal microbiota transplantation experiment was conducted to determine the role of the gut microbiota in ETEC-induced diarrhea. ETEC-induced diarrhea influenced the structure and function of gut microbiota. Diarrheal piglets had lower Bacteroidetes: Firmicutes ratio and microbiota diversity in the jejunum and feces, and lower percentage of Prevotella in the feces, but higher Lactococcus in the jejunum and higher Escherichia-Shigella in the feces. The transplantation of the jejunal microbiota from diarrheal piglets to uninfected piglets leaded to diarrhea after transplantation. Microbiota transplantation experiments also supported the notion that dysbiosis of gut microbiota is involved in the immune responses in ETEC-induced diarrhea. Conclusion We conclude that ETEC infection influences the gut microbiota and the dysbiosis of gut microbiota after ETEC infection mediates the immune responses in ETEC infection. |
| first_indexed | 2024-12-24T14:05:14Z |
| format | Article |
| id | doaj.art-b85988230bc14eb3a520471896332f32 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-24T14:05:14Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-b85988230bc14eb3a520471896332f322022-12-21T16:52:23ZengBMCBMC Veterinary Research1746-61482018-12-0114111310.1186/s12917-018-1704-9Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in pigletsPeng Bin0Zhiyi Tang1Shaojuan Liu2Shuai Chen3Yaoyao Xia4Jiaqi Liu5Hucong Wu6Guoqiang Zhu7Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Jiangsu Co-innovation Center for Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityCollege of Animal Science, South China Agricultural UniversityCollege of Animal Science, South China Agricultural UniversityLaboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of SciencesLaboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of SciencesJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Jiangsu Co-innovation Center for Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Jiangsu Co-innovation Center for Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Jiangsu Co-innovation Center for Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou UniversityAbstract Background Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in humans, cows, and pigs. The gut microbiota underlies pathology of several infectious diseases yet the role of the gut microbiota in the pathogenesis of ETEC-induced diarrhea is unknown. Results By using an ETEC induced diarrheal model in piglet, we profiled the jejunal and fecal microbiota using metagenomics and 16S rRNA sequencing. A jejunal microbiota transplantation experiment was conducted to determine the role of the gut microbiota in ETEC-induced diarrhea. ETEC-induced diarrhea influenced the structure and function of gut microbiota. Diarrheal piglets had lower Bacteroidetes: Firmicutes ratio and microbiota diversity in the jejunum and feces, and lower percentage of Prevotella in the feces, but higher Lactococcus in the jejunum and higher Escherichia-Shigella in the feces. The transplantation of the jejunal microbiota from diarrheal piglets to uninfected piglets leaded to diarrhea after transplantation. Microbiota transplantation experiments also supported the notion that dysbiosis of gut microbiota is involved in the immune responses in ETEC-induced diarrhea. Conclusion We conclude that ETEC infection influences the gut microbiota and the dysbiosis of gut microbiota after ETEC infection mediates the immune responses in ETEC infection.http://link.springer.com/article/10.1186/s12917-018-1704-9DiarrheaEnterotoxigenic Escherichia coliMicrobiotaMicrobiota transplantationPiglet |
| spellingShingle | Peng Bin Zhiyi Tang Shaojuan Liu Shuai Chen Yaoyao Xia Jiaqi Liu Hucong Wu Guoqiang Zhu Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets BMC Veterinary Research Diarrhea Enterotoxigenic Escherichia coli Microbiota Microbiota transplantation Piglet |
| title | Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets |
| title_full | Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets |
| title_fullStr | Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets |
| title_full_unstemmed | Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets |
| title_short | Intestinal microbiota mediates Enterotoxigenic Escherichia coli-induced diarrhea in piglets |
| title_sort | intestinal microbiota mediates enterotoxigenic escherichia coli induced diarrhea in piglets |
| topic | Diarrhea Enterotoxigenic Escherichia coli Microbiota Microbiota transplantation Piglet |
| url | http://link.springer.com/article/10.1186/s12917-018-1704-9 |
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