Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V
α-Series gangliosides define a particular sub-class of glycosphingolipids containing sialic acid α2,6-linked to GalNAc residue that was isolated as a minor compound from the brain. The sialyltransferase ST6GalNAc V was cloned from mouse brain and showed α2,6-sialyltransferase activity almost exclusi...
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| Format: | Article |
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MDPI AG
2015-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/4/6913 |
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| author | Sandy Vandermeersch Jorick Vanbeselaere Clément P. Delannoy Aurore Drolez Caroline Mysiorek Yann Guérardel Philippe Delannoy Sylvain Julien |
| author_facet | Sandy Vandermeersch Jorick Vanbeselaere Clément P. Delannoy Aurore Drolez Caroline Mysiorek Yann Guérardel Philippe Delannoy Sylvain Julien |
| author_sort | Sandy Vandermeersch |
| collection | DOAJ |
| description | α-Series gangliosides define a particular sub-class of glycosphingolipids containing sialic acid α2,6-linked to GalNAc residue that was isolated as a minor compound from the brain. The sialyltransferase ST6GalNAc V was cloned from mouse brain and showed α2,6-sialyltransferase activity almost exclusively for GM1b, to form GD1α and is considered as the main enzyme involved in the biosynthesis of α-series gangliosides. Recently, ST6GALNAC5 was identified as one of the genes over-expressed in breast cancer cell populations selected for their ability to produce brain metastasis. However, the capacity of human breast cancer cells to produce α-series gangliosides has never been clearly demonstrated. Here, we show by stable transfection and MS-MS analysis of total glycosphingolipids that ST6GALNAC5 expressing MDA-MB-231 breast cancer cells accumulate GD1α ganglioside (IV3Neu5Ac1, III6Neu5Ac1Gg4-Cer). |
| first_indexed | 2024-12-10T14:25:01Z |
| format | Article |
| id | doaj.art-b85c989996e74798844de49b07e9ffed |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T14:25:01Z |
| publishDate | 2015-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b85c989996e74798844de49b07e9ffed2022-12-22T01:45:06ZengMDPI AGMolecules1420-30492015-04-012046913692410.3390/molecules20046913molecules20046913Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc VSandy Vandermeersch0Jorick Vanbeselaere1Clément P. Delannoy2Aurore Drolez3Caroline Mysiorek4Yann Guérardel5Philippe Delannoy6Sylvain Julien7Structural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, FranceStructural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, FranceStructural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, FranceBlood Brain Barrier Laboratory (LBHE), EA 2465, University of Artois, 62300 Lens, FranceBlood Brain Barrier Laboratory (LBHE), EA 2465, University of Artois, 62300 Lens, FranceStructural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, FranceStructural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, FranceStructural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, Franceα-Series gangliosides define a particular sub-class of glycosphingolipids containing sialic acid α2,6-linked to GalNAc residue that was isolated as a minor compound from the brain. The sialyltransferase ST6GalNAc V was cloned from mouse brain and showed α2,6-sialyltransferase activity almost exclusively for GM1b, to form GD1α and is considered as the main enzyme involved in the biosynthesis of α-series gangliosides. Recently, ST6GALNAC5 was identified as one of the genes over-expressed in breast cancer cell populations selected for their ability to produce brain metastasis. However, the capacity of human breast cancer cells to produce α-series gangliosides has never been clearly demonstrated. Here, we show by stable transfection and MS-MS analysis of total glycosphingolipids that ST6GALNAC5 expressing MDA-MB-231 breast cancer cells accumulate GD1α ganglioside (IV3Neu5Ac1, III6Neu5Ac1Gg4-Cer).http://www.mdpi.com/1420-3049/20/4/6913ST6GalNAc Vbreast cancerMDA-MB-231α-gangliosidesGD1α |
| spellingShingle | Sandy Vandermeersch Jorick Vanbeselaere Clément P. Delannoy Aurore Drolez Caroline Mysiorek Yann Guérardel Philippe Delannoy Sylvain Julien Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V Molecules ST6GalNAc V breast cancer MDA-MB-231 α-gangliosides GD1α |
| title | Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V |
| title_full | Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V |
| title_fullStr | Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V |
| title_full_unstemmed | Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V |
| title_short | Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V |
| title_sort | accumulation of gd1α ganglioside in mda mb 231 breast cancer cells expressing st6galnac v |
| topic | ST6GalNAc V breast cancer MDA-MB-231 α-gangliosides GD1α |
| url | http://www.mdpi.com/1420-3049/20/4/6913 |
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