Testing human sperm chemotaxis: how to detect biased motion in population assays.
Biased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3297605?pdf=render |
| _version_ | 1828850198268346368 |
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| author | Leah Armon S Roy Caplan Michael Eisenbach Benjamin M Friedrich |
| author_facet | Leah Armon S Roy Caplan Michael Eisenbach Benjamin M Friedrich |
| author_sort | Leah Armon |
| collection | DOAJ |
| description | Biased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle differences. In these assays, statistical measures such as population odds ratios of swimming directions can be employed to infer chemotactic performance. Here, we report on an improved method to assess statistical significance of experimentally determined odds ratios and discuss the strong impact of data correlations that arise from the directional persistence of sperm swimming. |
| first_indexed | 2024-12-12T23:06:54Z |
| format | Article |
| id | doaj.art-b85cec7c757d47c5ab0decafa7f722fc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T23:06:54Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b85cec7c757d47c5ab0decafa7f722fc2022-12-22T00:08:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3290910.1371/journal.pone.0032909Testing human sperm chemotaxis: how to detect biased motion in population assays.Leah ArmonS Roy CaplanMichael EisenbachBenjamin M FriedrichBiased motion of motile cells in a concentration gradient of a chemoattractant is frequently studied on the population level. This approach has been particularly employed in human sperm chemotactic assays, where the fraction of responsive cells is low and detection of biased motion depends on subtle differences. In these assays, statistical measures such as population odds ratios of swimming directions can be employed to infer chemotactic performance. Here, we report on an improved method to assess statistical significance of experimentally determined odds ratios and discuss the strong impact of data correlations that arise from the directional persistence of sperm swimming.http://europepmc.org/articles/PMC3297605?pdf=render |
| spellingShingle | Leah Armon S Roy Caplan Michael Eisenbach Benjamin M Friedrich Testing human sperm chemotaxis: how to detect biased motion in population assays. PLoS ONE |
| title | Testing human sperm chemotaxis: how to detect biased motion in population assays. |
| title_full | Testing human sperm chemotaxis: how to detect biased motion in population assays. |
| title_fullStr | Testing human sperm chemotaxis: how to detect biased motion in population assays. |
| title_full_unstemmed | Testing human sperm chemotaxis: how to detect biased motion in population assays. |
| title_short | Testing human sperm chemotaxis: how to detect biased motion in population assays. |
| title_sort | testing human sperm chemotaxis how to detect biased motion in population assays |
| url | http://europepmc.org/articles/PMC3297605?pdf=render |
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