Revisiting Suppression of Interspecies Hybrid Male Lethality in Caenorhabditis Nematodes
Within the nematode genus Caenorhabditis, Caenorhabditis briggsae and C. nigoni are among the most closely related species known. They differ in sexual mode, with C. nigoni retaining the ancestral XO male–XX female outcrossing system, while C. briggsae recently evolved self-fertility and an XX-biase...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2017-04-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.117.039479 |
| _version_ | 1818652730612252672 |
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| author | Lauren E. Ryan Eric S. Haag |
| author_facet | Lauren E. Ryan Eric S. Haag |
| author_sort | Lauren E. Ryan |
| collection | DOAJ |
| description | Within the nematode genus Caenorhabditis, Caenorhabditis briggsae and C. nigoni are among the most closely related species known. They differ in sexual mode, with C. nigoni retaining the ancestral XO male–XX female outcrossing system, while C. briggsae recently evolved self-fertility and an XX-biased sex ratio. Wild-type C. briggsae and C. nigoni can produce fertile hybrid XX female progeny, but XO progeny are either 100% inviable (when C. briggsae is the mother) or viable but sterile (when C. nigoni is the mother). A recent study provided evidence suggesting that loss of the Cbr-him-8 meiotic regulator in C. briggsae hermaphrodites allowed them to produce viable and fertile hybrid XO male progeny when mated to C. nigoni. Because such males would be useful for a variety of genetic experiments, we sought to verify this result. Preliminary crosses with wild-type C. briggsae hermaphrodites occasionally produced fertile males, but they could not be confirmed to be interspecies hybrids. Using an RNA interference (RNAi) protocol that eliminates any possibility of self-progeny in Cbr-him-8 hermaphrodites, we found sterile males bearing the C. nigoni X chromosome, but no fertile males bearing the C. briggsae X, as in wild-type crosses. Our results suggest that the apparent rescue of XO hybrid viability and fertility is due to incomplete purging of self-sperm prior to mating. |
| first_indexed | 2024-12-17T02:26:39Z |
| format | Article |
| id | doaj.art-b85e3446fd5b45af9a55d16699bdce15 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-17T02:26:39Z |
| publishDate | 2017-04-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-b85e3446fd5b45af9a55d16699bdce152022-12-21T22:07:05ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362017-04-01741211121410.1534/g3.117.03947914Revisiting Suppression of Interspecies Hybrid Male Lethality in Caenorhabditis NematodesLauren E. RyanEric S. HaagWithin the nematode genus Caenorhabditis, Caenorhabditis briggsae and C. nigoni are among the most closely related species known. They differ in sexual mode, with C. nigoni retaining the ancestral XO male–XX female outcrossing system, while C. briggsae recently evolved self-fertility and an XX-biased sex ratio. Wild-type C. briggsae and C. nigoni can produce fertile hybrid XX female progeny, but XO progeny are either 100% inviable (when C. briggsae is the mother) or viable but sterile (when C. nigoni is the mother). A recent study provided evidence suggesting that loss of the Cbr-him-8 meiotic regulator in C. briggsae hermaphrodites allowed them to produce viable and fertile hybrid XO male progeny when mated to C. nigoni. Because such males would be useful for a variety of genetic experiments, we sought to verify this result. Preliminary crosses with wild-type C. briggsae hermaphrodites occasionally produced fertile males, but they could not be confirmed to be interspecies hybrids. Using an RNA interference (RNAi) protocol that eliminates any possibility of self-progeny in Cbr-him-8 hermaphrodites, we found sterile males bearing the C. nigoni X chromosome, but no fertile males bearing the C. briggsae X, as in wild-type crosses. Our results suggest that the apparent rescue of XO hybrid viability and fertility is due to incomplete purging of self-sperm prior to mating.http://g3journal.org/lookup/doi/10.1534/g3.117.039479Haldane’s ruleX chromosomehybridsGenetics of Sex |
| spellingShingle | Lauren E. Ryan Eric S. Haag Revisiting Suppression of Interspecies Hybrid Male Lethality in Caenorhabditis Nematodes G3: Genes, Genomes, Genetics Haldane’s rule X chromosome hybrids Genetics of Sex |
| title | Revisiting Suppression of Interspecies Hybrid Male Lethality in Caenorhabditis Nematodes |
| title_full | Revisiting Suppression of Interspecies Hybrid Male Lethality in Caenorhabditis Nematodes |
| title_fullStr | Revisiting Suppression of Interspecies Hybrid Male Lethality in Caenorhabditis Nematodes |
| title_full_unstemmed | Revisiting Suppression of Interspecies Hybrid Male Lethality in Caenorhabditis Nematodes |
| title_short | Revisiting Suppression of Interspecies Hybrid Male Lethality in Caenorhabditis Nematodes |
| title_sort | revisiting suppression of interspecies hybrid male lethality in caenorhabditis nematodes |
| topic | Haldane’s rule X chromosome hybrids Genetics of Sex |
| url | http://g3journal.org/lookup/doi/10.1534/g3.117.039479 |
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