Genotyping of Pathogenic Mycoplasma bovis Isolated from Cattle in Kafr El-Sheikh Province, Egypt
Mycoplasma bovis is one of the important pathogens in mycoplasma types that cause disease in cattle. The young calves from one to four months of age are most likely to develop pneumonia caused by M. bovis. In this study, we isolated M. bovis from tracheal swabs of cattle which showed respiratory s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2018-12-01
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| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/genotyping-of-pathogenic-mycoplasma-bovis-isolated-from-cattle-in-kafr-el-sheikh-province-egypt/ |
| Summary: | Mycoplasma bovis is one of the important pathogens in mycoplasma types that cause disease in cattle. The young
calves from one to four months of age are most likely to develop pneumonia caused by M. bovis. In this study, we
isolated M. bovis from tracheal swabs of cattle which showed respiratory symptoms. A total of about 100 tracheal
swab samples were collected from cattle in Kafer El-Shikh slaughterhouse, Egypt. The collected samples from cattle
were between 3-12 months of age. Mycoplasma bovis was identification in tracheal swab samples by using 16S rDNA
gene sequencing and biotyping by using rep-PCR, respectively. The microbiological method could not give positive
results, while the PCR showed that M. bovis infections were positive in 16 different cattle samples with about 16%.
The partial sequences of the 16S rRNA genes of the Mycoplasma isolates were obtained and phylogeny tree showed
that Sixteen Mycoplasma isolates were identified into Mycoplasma bovis. the similarity to Mycoplasma bovis
MYC 84, M. bovis L22 and M. bovis MYC 76 was 100, 99 and 95%, respectively. The ten Rep-PCR primers produced
about 139 fragments, 53.3% of them consider as monomorphic and 46.7% of them consider as polymorphic bands.
According to genetic similarity and intraspecies differentiation, the sixteen Mycoplasma isolates were grouped into
two main different clusters with about 60% genetic similarity in genetics dendrogram. These results suggest that
PCR technique is a specific molecular detection technique identified to determine Mycoplasma and It is easy and
fast methods to detect and isolate infected animals. |
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| ISSN: | 0973-7510 2581-690X |