Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells
Classical Hodgkin’s lymphoma (cHL) is a B-Cell lymphoma comprised of mononuclear Hodgkin cells (H) and bi- to multi-nucleated Reed-Sternberg (RS) cells. Previous studies revealed that H and RS cells express lamin A/C, a component of the lamina of the nuclear matrix. Since no information wa...
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MDPI AG
2018-08-01
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| Series: | Cancers |
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| Online Access: | http://www.mdpi.com/2072-6694/10/9/286 |
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| author | Fabio Contu Aline Rangel-Pozzo Peter Trokajlo Landon Wark Ludger Klewes Nathalie A. Johnson Tina Petrogiannis-Haliotis John G. Gartner Yuval Garini Roberta Vanni Hans Knecht Sabine Mai |
| author_facet | Fabio Contu Aline Rangel-Pozzo Peter Trokajlo Landon Wark Ludger Klewes Nathalie A. Johnson Tina Petrogiannis-Haliotis John G. Gartner Yuval Garini Roberta Vanni Hans Knecht Sabine Mai |
| author_sort | Fabio Contu |
| collection | DOAJ |
| description | Classical Hodgkin’s lymphoma (cHL) is a B-Cell lymphoma comprised of mononuclear Hodgkin cells (H) and bi- to multi-nucleated Reed-Sternberg (RS) cells. Previous studies revealed that H and RS cells express lamin A/C, a component of the lamina of the nuclear matrix. Since no information was available about the three-dimensional (3D) expression patterns of lamin A/C in H and RS cells, we analyzed the 3D spatial organization of lamin in such cells, using 3D fluorescent microscopy. H and RS cells from cHL derived cell lines stained positive for lamin A/C, in contrast to peripheral blood lymphocytes (PBLs), in which the lamin A/C protein was not detected or weak, although its presence could be transiently increased with lymphocyte activation by lipopolysaccharide (LPS). Most importantly, in H and RS cells, the regular homogeneous and spherically shaped lamin A/C pattern, identified in activated lymphocytes, was absent. Instead, in H and RS cells, lamin staining showed internal lamin A/C structures, subdividing the nuclei into two or more smaller compartments. Analysis of pre-treatment cHL patients’ samples replicated the lamin patterns identified in cHL cell lines. We conclude that the investigation of lamin A/C protein could be a useful tool for understanding nuclear remodeling in cHL. |
| first_indexed | 2024-03-12T05:43:47Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T05:43:47Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-b8627964def1476ca5f16761386bb2942023-09-03T05:51:11ZengMDPI AGCancers2072-66942018-08-0110928610.3390/cancers10090286cancers10090286Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg CellsFabio Contu0Aline Rangel-Pozzo1Peter Trokajlo2Landon Wark3Ludger Klewes4Nathalie A. Johnson5Tina Petrogiannis-Haliotis6John G. Gartner7Yuval Garini8Roberta Vanni9Hans Knecht10Sabine Mai11Cell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, CanadaCell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, CanadaCell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, CanadaCell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, CanadaCell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, CanadaDivision of Hematology and Division of Pathology, Department of Medicine, Jewish General Hospital, McGill University, Montréal, QC H3G 2M1, CanadaDivision of Hematology and Division of Pathology, Department of Medicine, Jewish General Hospital, McGill University, Montréal, QC H3G 2M1, CanadaDepartment of Pathology, University of Manitoba, Winnipeg, MB R3E 0W2, CanadaPhysics Department & Institute of Nanotechnology, Bar Ilan University, Ramat Gan 5290002, IsraelDepartment of Biomedical Sciences, Unit of Biology and Genetics, University of Cagliari, 09042 Cagliari, ItalyDivision of Hematology and Division of Pathology, Department of Medicine, Jewish General Hospital, McGill University, Montréal, QC H3G 2M1, CanadaCell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, CanadaClassical Hodgkin’s lymphoma (cHL) is a B-Cell lymphoma comprised of mononuclear Hodgkin cells (H) and bi- to multi-nucleated Reed-Sternberg (RS) cells. Previous studies revealed that H and RS cells express lamin A/C, a component of the lamina of the nuclear matrix. Since no information was available about the three-dimensional (3D) expression patterns of lamin A/C in H and RS cells, we analyzed the 3D spatial organization of lamin in such cells, using 3D fluorescent microscopy. H and RS cells from cHL derived cell lines stained positive for lamin A/C, in contrast to peripheral blood lymphocytes (PBLs), in which the lamin A/C protein was not detected or weak, although its presence could be transiently increased with lymphocyte activation by lipopolysaccharide (LPS). Most importantly, in H and RS cells, the regular homogeneous and spherically shaped lamin A/C pattern, identified in activated lymphocytes, was absent. Instead, in H and RS cells, lamin staining showed internal lamin A/C structures, subdividing the nuclei into two or more smaller compartments. Analysis of pre-treatment cHL patients’ samples replicated the lamin patterns identified in cHL cell lines. We conclude that the investigation of lamin A/C protein could be a useful tool for understanding nuclear remodeling in cHL.http://www.mdpi.com/2072-6694/10/9/286Hodgkin’s LymphomaReed-Sternberg cellsLamin A/C patterns3D imagingLymphocytesnuclear architecture |
| spellingShingle | Fabio Contu Aline Rangel-Pozzo Peter Trokajlo Landon Wark Ludger Klewes Nathalie A. Johnson Tina Petrogiannis-Haliotis John G. Gartner Yuval Garini Roberta Vanni Hans Knecht Sabine Mai Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells Cancers Hodgkin’s Lymphoma Reed-Sternberg cells Lamin A/C patterns 3D imaging Lymphocytes nuclear architecture |
| title | Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
| title_full | Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
| title_fullStr | Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
| title_full_unstemmed | Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
| title_short | Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
| title_sort | distinct 3d structural patterns of lamin a c expression in hodgkin and reed sternberg cells |
| topic | Hodgkin’s Lymphoma Reed-Sternberg cells Lamin A/C patterns 3D imaging Lymphocytes nuclear architecture |
| url | http://www.mdpi.com/2072-6694/10/9/286 |
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