Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis
Abstract Background Innate lymphoid cells (ILCs) play an essential role in maintaining homeostasis; however, they can also cause chronic inflammation and autoimmune disease. This study aimed to identify the role of ILCs in the pathogenesis of lupus nephritis (LN). Methods The percentage of ILCs with...
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BMC
2020-03-01
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| Series: | Arthritis Research & Therapy |
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| Online Access: | http://link.springer.com/article/10.1186/s13075-020-2114-5 |
| _version_ | 1818776036951719936 |
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| author | Seungwon Ryu Eun Young Lee Dong Ki Kim Yon Su Kim Doo Hyun Chung Ji Hyung Kim Hajeong Lee Hye Young Kim |
| author_facet | Seungwon Ryu Eun Young Lee Dong Ki Kim Yon Su Kim Doo Hyun Chung Ji Hyung Kim Hajeong Lee Hye Young Kim |
| author_sort | Seungwon Ryu |
| collection | DOAJ |
| description | Abstract Background Innate lymphoid cells (ILCs) play an essential role in maintaining homeostasis; however, they can also cause chronic inflammation and autoimmune disease. This study aimed to identify the role of ILCs in the pathogenesis of lupus nephritis (LN). Methods The percentage of ILCs within the peripheral blood mononuclear cell (PBMC) population and urine of patients with LN (n = 16), healthy controls (HC; n = 8), and disease controls (ANCA-associated vasculitis (AAV; n = 6), IgA nephropathy (IgAN; n = 9), and other glomerular diseases (n = 5)) was determined by flow cytometry analysis. In addition, ILCs were sorted and cultured with plasma from LN patients or HC to elucidate whether the reduced population of CD117+ ILCs observed in LN was due to changes in the ILC progenitor population. Results The percentage of total ILCs and CD117+ ILCs in LN was significantly lower than that in HC. The percentage of cytokine-secreting ILCs was also lower in LN; however, when the disease stabilized, cytokine production was restored to levels similar to those in HC. The increase in the number of exhausted ILCs (cells unable to secrete cytokines) correlated positively with disease activity. When CD117+ ILCs were cultured with LN plasma, the number of CD117+ ILCs fell, but that of other ILC subsets increased. Conclusions The percentage of CD117+ ILCs and the capacity of ILCs to secrete cytokines fell as LN severity increased, suggesting that an inflammatory environment of LN induces persistent differentiation and exhaustion of ILCs. |
| first_indexed | 2024-12-18T11:06:33Z |
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| institution | Directory Open Access Journal |
| issn | 1478-6362 |
| language | English |
| last_indexed | 2024-12-18T11:06:33Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
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| series | Arthritis Research & Therapy |
| spelling | doaj.art-b862ec52ffda4f71a37c82ad31d9248a2022-12-21T21:10:05ZengBMCArthritis Research & Therapy1478-63622020-03-0122111310.1186/s13075-020-2114-5Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritisSeungwon Ryu0Eun Young Lee1Dong Ki Kim2Yon Su Kim3Doo Hyun Chung4Ji Hyung Kim5Hajeong Lee6Hye Young Kim7Laboratory of Mucosal Immunology, Department of Biomedical Sciences, Seoul National University College of MedicineDivision of Rheumatology, Department of Internal Medicine, Seoul National University HospitalDivision of Nephrology, Department of Internal Medicine, Seoul National University HospitalDivision of Nephrology, Department of Internal Medicine, Seoul National University HospitalDepartment of Pathology, Seoul National University College of MedicineCollege of Life Sciences and Biotechnology, Korea UniversityDivision of Nephrology, Department of Internal Medicine, Seoul National University HospitalLaboratory of Mucosal Immunology, Department of Biomedical Sciences, Seoul National University College of MedicineAbstract Background Innate lymphoid cells (ILCs) play an essential role in maintaining homeostasis; however, they can also cause chronic inflammation and autoimmune disease. This study aimed to identify the role of ILCs in the pathogenesis of lupus nephritis (LN). Methods The percentage of ILCs within the peripheral blood mononuclear cell (PBMC) population and urine of patients with LN (n = 16), healthy controls (HC; n = 8), and disease controls (ANCA-associated vasculitis (AAV; n = 6), IgA nephropathy (IgAN; n = 9), and other glomerular diseases (n = 5)) was determined by flow cytometry analysis. In addition, ILCs were sorted and cultured with plasma from LN patients or HC to elucidate whether the reduced population of CD117+ ILCs observed in LN was due to changes in the ILC progenitor population. Results The percentage of total ILCs and CD117+ ILCs in LN was significantly lower than that in HC. The percentage of cytokine-secreting ILCs was also lower in LN; however, when the disease stabilized, cytokine production was restored to levels similar to those in HC. The increase in the number of exhausted ILCs (cells unable to secrete cytokines) correlated positively with disease activity. When CD117+ ILCs were cultured with LN plasma, the number of CD117+ ILCs fell, but that of other ILC subsets increased. Conclusions The percentage of CD117+ ILCs and the capacity of ILCs to secrete cytokines fell as LN severity increased, suggesting that an inflammatory environment of LN induces persistent differentiation and exhaustion of ILCs.http://link.springer.com/article/10.1186/s13075-020-2114-5Lupus nephritisAutoimmune kidney diseaseInnate lymphoid cells (ILCs)Cytokine |
| spellingShingle | Seungwon Ryu Eun Young Lee Dong Ki Kim Yon Su Kim Doo Hyun Chung Ji Hyung Kim Hajeong Lee Hye Young Kim Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis Arthritis Research & Therapy Lupus nephritis Autoimmune kidney disease Innate lymphoid cells (ILCs) Cytokine |
| title | Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
| title_full | Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
| title_fullStr | Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
| title_full_unstemmed | Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
| title_short | Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
| title_sort | reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
| topic | Lupus nephritis Autoimmune kidney disease Innate lymphoid cells (ILCs) Cytokine |
| url | http://link.springer.com/article/10.1186/s13075-020-2114-5 |
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