Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.)
Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular mechanisms responsible for drought stress resp...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2018-05-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/4752.pdf |
| _version_ | 1797420214644637696 |
|---|---|
| author | Weiping Shi Jingye Cheng Xiaojie Wen Jixiang Wang Guanyan Shi Jiayan Yao Liyuan Hou Qian Sun Peng Xiang Xiangyang Yuan Shuqi Dong Pingyi Guo Jie Guo |
| author_facet | Weiping Shi Jingye Cheng Xiaojie Wen Jixiang Wang Guanyan Shi Jiayan Yao Liyuan Hou Qian Sun Peng Xiang Xiangyang Yuan Shuqi Dong Pingyi Guo Jie Guo |
| author_sort | Weiping Shi |
| collection | DOAJ |
| description | Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular mechanisms responsible for drought stress responses is vital to improvement of agricultural production. In this study, we exposed the drought resistant F1 hybrid, M79, and its parental lines E1 and H1 to drought stress. Subsequent physiological analysis demonstrated that M79 showed higher photosynthetic energy conversion efficiency and drought tolerance than its parents. A transcriptomic study using leaves collected six days after drought treatment, when the soil water content was about ∼20%, identified 3066, 1895, and 2148 differentially expressed genes (DEGs) in M79, E1 and H1 compared to the respective untreated controls, respectively. Further analysis revealed 17 Gene Ontology (GO) enrichments and 14 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in M79, including photosystem II (PSII) oxygen-evolving complex, peroxidase (POD) activity, plant hormone signal transduction, and chlorophyll biosynthesis. Co-regulation analysis suggested that these DEGs in M79 contributed to the formation of a regulatory network involving multiple biological processes and pathways including photosynthesis, signal transduction, transcriptional regulation, redox regulation, hormonal signaling, and osmotic regulation. RNA-seq analysis also showed that some photosynthesis-related DEGs were highly expressed in M79 compared to its parental lines under drought stress. These results indicate that various molecular pathways, including photosynthesis, respond to drought stress in M79, and provide abundant molecular information for further analysis of the underlying mechanism responding to this stress. |
| first_indexed | 2024-03-09T06:59:25Z |
| format | Article |
| id | doaj.art-b86351eb285c4a2bb2d27aaa2d20aca7 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:59:25Z |
| publishDate | 2018-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-b86351eb285c4a2bb2d27aaa2d20aca72023-12-03T09:56:51ZengPeerJ Inc.PeerJ2167-83592018-05-016e475210.7717/peerj.4752Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.)Weiping Shi0Jingye Cheng1Xiaojie Wen2Jixiang Wang3Guanyan Shi4Jiayan Yao5Liyuan Hou6Qian Sun7Peng Xiang8Xiangyang Yuan9Shuqi Dong10Pingyi Guo11Jie Guo12College of Agronomy, Shanxi Agricultural University, Taigu, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, ChinaBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu, ChinaIndustrial Crop Institute, Shanxi Academy of Agricultural Sciences, Fenyang, ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu, ChinaDepartment of Next Generation Sequencing, Vazyme Biotech Company Ltd., Nanjing, ChinaDepartment of Next Generation Sequencing, Vazyme Biotech Company Ltd., Nanjing, ChinaDepartment of Next Generation Sequencing, Vazyme Biotech Company Ltd., Nanjing, ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu, ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu, ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu, ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu, ChinaDrought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular mechanisms responsible for drought stress responses is vital to improvement of agricultural production. In this study, we exposed the drought resistant F1 hybrid, M79, and its parental lines E1 and H1 to drought stress. Subsequent physiological analysis demonstrated that M79 showed higher photosynthetic energy conversion efficiency and drought tolerance than its parents. A transcriptomic study using leaves collected six days after drought treatment, when the soil water content was about ∼20%, identified 3066, 1895, and 2148 differentially expressed genes (DEGs) in M79, E1 and H1 compared to the respective untreated controls, respectively. Further analysis revealed 17 Gene Ontology (GO) enrichments and 14 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in M79, including photosystem II (PSII) oxygen-evolving complex, peroxidase (POD) activity, plant hormone signal transduction, and chlorophyll biosynthesis. Co-regulation analysis suggested that these DEGs in M79 contributed to the formation of a regulatory network involving multiple biological processes and pathways including photosynthesis, signal transduction, transcriptional regulation, redox regulation, hormonal signaling, and osmotic regulation. RNA-seq analysis also showed that some photosynthesis-related DEGs were highly expressed in M79 compared to its parental lines under drought stress. These results indicate that various molecular pathways, including photosynthesis, respond to drought stress in M79, and provide abundant molecular information for further analysis of the underlying mechanism responding to this stress.https://peerj.com/articles/4752.pdfCo-regulation networkDrought tolerancePhotosynthesisRNA sequencingFoxtail millet (Setaria italica L.) |
| spellingShingle | Weiping Shi Jingye Cheng Xiaojie Wen Jixiang Wang Guanyan Shi Jiayan Yao Liyuan Hou Qian Sun Peng Xiang Xiangyang Yuan Shuqi Dong Pingyi Guo Jie Guo Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) PeerJ Co-regulation network Drought tolerance Photosynthesis RNA sequencing Foxtail millet (Setaria italica L.) |
| title | Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) |
| title_full | Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) |
| title_fullStr | Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) |
| title_full_unstemmed | Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) |
| title_short | Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) |
| title_sort | transcriptomic studies reveal a key metabolic pathway contributing to a well maintained photosynthetic system under drought stress in foxtail millet setaria italica l |
| topic | Co-regulation network Drought tolerance Photosynthesis RNA sequencing Foxtail millet (Setaria italica L.) |
| url | https://peerj.com/articles/4752.pdf |
| work_keys_str_mv | AT weipingshi transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT jingyecheng transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT xiaojiewen transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT jixiangwang transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT guanyanshi transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT jiayanyao transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT liyuanhou transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT qiansun transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT pengxiang transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT xiangyangyuan transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT shuqidong transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT pingyiguo transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical AT jieguo transcriptomicstudiesrevealakeymetabolicpathwaycontributingtoawellmaintainedphotosyntheticsystemunderdroughtstressinfoxtailmilletsetariaitalical |