In vitro activity of anti-rheumatic drugs on release of pro-inflammatory cytokines from oral cells in interaction with microorganisms
Periodontitis patients suffering concomitantly from rheumatoid arthritis (RA) often present with less inflamed periodontal tissues due to the ongoing anti-rheumatic therapy. This in vitro study was aimed to analyze whether anti-inflammatory drugs used in the therapy of RA can modulate the release of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Oral Health |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/froh.2022.960732/full |
| _version_ | 1797858500836065280 |
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| author | Alexandra Stähli Carina Scherler Graziano Zappalà Anton Sculean Sigrun Eick |
| author_facet | Alexandra Stähli Carina Scherler Graziano Zappalà Anton Sculean Sigrun Eick |
| author_sort | Alexandra Stähli |
| collection | DOAJ |
| description | Periodontitis patients suffering concomitantly from rheumatoid arthritis (RA) often present with less inflamed periodontal tissues due to the ongoing anti-rheumatic therapy. This in vitro study was aimed to analyze whether anti-inflammatory drugs used in the therapy of RA can modulate the release of IL-8 and IL-1β by professional and non-professional immune cells stimulated with microorganisms. Periodontal ligament (PDL) fibroblasts, monocytic MONO-MAC-6-cells, and gingival keratinocytes were exposed to ibuprofen, prednisolone, and methotrexate with and without lysates of Fusobacterium nucleatum or Candida albicans. Supernatants were obtained and the levels of interleukin(IL)-8 and IL-1β (only MONO-MAC-6) were quantified. The addition of F. nucleatum lysate resulted in the strongest release of proinflammatory cytokines by PDL fibroblast and MONO-MAC-6 cells, while the modification by the tested anti-rheumatic drugs was only minor. After stimulation of the MONO-MAC-cells with F. nucleatum, prednisolone increased the release of IL-8, whereas methotrexate decreased the level. Anti-inflammatory drugs increased the adherence of C. albicans to epithelial cells. In patients with RA, the reduction of the microbial load in subgingival biofilm (biofilm removal) is of major importance; however, the intake of inflammatory drugs may interfere with the inflammatory response. |
| first_indexed | 2024-04-09T21:14:23Z |
| format | Article |
| id | doaj.art-b86409360ca5433e8cbfb012342ae2ce |
| institution | Directory Open Access Journal |
| issn | 2673-4842 |
| language | English |
| last_indexed | 2024-04-09T21:14:23Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oral Health |
| spelling | doaj.art-b86409360ca5433e8cbfb012342ae2ce2023-03-28T12:25:30ZengFrontiers Media S.A.Frontiers in Oral Health2673-48422022-09-01310.3389/froh.2022.960732960732In vitro activity of anti-rheumatic drugs on release of pro-inflammatory cytokines from oral cells in interaction with microorganismsAlexandra StähliCarina ScherlerGraziano ZappalàAnton SculeanSigrun EickPeriodontitis patients suffering concomitantly from rheumatoid arthritis (RA) often present with less inflamed periodontal tissues due to the ongoing anti-rheumatic therapy. This in vitro study was aimed to analyze whether anti-inflammatory drugs used in the therapy of RA can modulate the release of IL-8 and IL-1β by professional and non-professional immune cells stimulated with microorganisms. Periodontal ligament (PDL) fibroblasts, monocytic MONO-MAC-6-cells, and gingival keratinocytes were exposed to ibuprofen, prednisolone, and methotrexate with and without lysates of Fusobacterium nucleatum or Candida albicans. Supernatants were obtained and the levels of interleukin(IL)-8 and IL-1β (only MONO-MAC-6) were quantified. The addition of F. nucleatum lysate resulted in the strongest release of proinflammatory cytokines by PDL fibroblast and MONO-MAC-6 cells, while the modification by the tested anti-rheumatic drugs was only minor. After stimulation of the MONO-MAC-cells with F. nucleatum, prednisolone increased the release of IL-8, whereas methotrexate decreased the level. Anti-inflammatory drugs increased the adherence of C. albicans to epithelial cells. In patients with RA, the reduction of the microbial load in subgingival biofilm (biofilm removal) is of major importance; however, the intake of inflammatory drugs may interfere with the inflammatory response.https://www.frontiersin.org/articles/10.3389/froh.2022.960732/fullperiodontitisrheumatoid arthritisanti-inflammatory drugsproinflammatory cytokinesoral cells |
| spellingShingle | Alexandra Stähli Carina Scherler Graziano Zappalà Anton Sculean Sigrun Eick In vitro activity of anti-rheumatic drugs on release of pro-inflammatory cytokines from oral cells in interaction with microorganisms Frontiers in Oral Health periodontitis rheumatoid arthritis anti-inflammatory drugs proinflammatory cytokines oral cells |
| title | In vitro activity of anti-rheumatic drugs on release of pro-inflammatory cytokines from oral cells in interaction with microorganisms |
| title_full | In vitro activity of anti-rheumatic drugs on release of pro-inflammatory cytokines from oral cells in interaction with microorganisms |
| title_fullStr | In vitro activity of anti-rheumatic drugs on release of pro-inflammatory cytokines from oral cells in interaction with microorganisms |
| title_full_unstemmed | In vitro activity of anti-rheumatic drugs on release of pro-inflammatory cytokines from oral cells in interaction with microorganisms |
| title_short | In vitro activity of anti-rheumatic drugs on release of pro-inflammatory cytokines from oral cells in interaction with microorganisms |
| title_sort | in vitro activity of anti rheumatic drugs on release of pro inflammatory cytokines from oral cells in interaction with microorganisms |
| topic | periodontitis rheumatoid arthritis anti-inflammatory drugs proinflammatory cytokines oral cells |
| url | https://www.frontiersin.org/articles/10.3389/froh.2022.960732/full |
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