An improved plant CRISPR-Cas9 system for generating Cas9-free multiplex mutants

An improved plant CRISPR-Cas9 system for generating Cas9-free multiplex mutants

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 mediated gene editing systems have been used in a variety of organisms, including plants. This system can efficiently edit target genes to generate mutants, especially multiplex mutants, which are invaluable genetic materials fo...

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Bibliographic Details
Main Authors: Jun-Li Wang, Luo-Yu Liang, Nan Lang, Yu-Yang Li, Wang Guo, Jian Cui, Ya-Jie Zou, Hai-Qin Liu, Qiong-Hui Fei, Xiao-Feng Li, Guang-Qin Guo, Lei Wu
Format: Article
Language:English
Published: Taylor & Francis Group 2021-01-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
crispr-cas9
multiplex genome editing
in-fusion cloning technology
cas9-free
Online Access:http://dx.doi.org/10.1080/13102818.2021.1986132

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