Partial biological and molecular characterization of Iranian isolates of Tomato aspermy virus
<em>Tomato aspermy virus</em> (TAV) has a wide host range and usually infects ornamental plants. To identify and characterize molecular and biological properties of the virus, 436 samples were collected from various provinces of Iran. TAV was detected in three samples of ornamental plant...
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| Format: | Article |
| Language: | fas |
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Shahid Bahonar University of Kerman
2012-08-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_468_1583d6c0780a7f7705dba14d30855150.pdf |
| _version_ | 1818152560249274368 |
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| author | Mohammad Madahian Hossein Masoomi |
| author_facet | Mohammad Madahian Hossein Masoomi |
| author_sort | Mohammad Madahian |
| collection | DOAJ |
| description | <em>Tomato aspermy virus</em> (TAV) has a wide host range and usually infects ornamental plants. To identify and characterize molecular and biological properties of the virus, 436 samples were collected from various provinces of Iran. TAV was detected in three samples of ornamental plants through double antibody sandwich ELISA test using specific polyclonal antibody. The 687 bp segment including the coat protein (CP) gene was amplified by RT-PCR, cloned and sequenced. Nucleotide sequences of the CP gene of Iranian TAV isolates were compared with available GenBank isolates. Phylogenetic analysis showed that TAV isolates were classified into two groups I and II, and the group II was divided in two subgroups IIA and IIB. All the Iranian TAV isolates were classified in subgroup IIA and shared the high nucleotide and amino acid sequence identities (more than 99%). The results of this study also showed a wide host range of the Iranian TAV isolates among test plants. This study is the first report of TAV occurrence in Iran and the first natural occurrence of TAV on <em>Petunia hybrida</em> in the world. |
| first_indexed | 2024-12-11T13:56:39Z |
| format | Article |
| id | doaj.art-b8661883c1b34415a5cafa46bb1f540d |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-11T13:56:39Z |
| publishDate | 2012-08-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-b8661883c1b34415a5cafa46bb1f540d2022-12-22T01:04:02ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002012-08-0141658110.22103/jab.2012.468468Partial biological and molecular characterization of Iranian isolates of Tomato aspermy virusMohammad Madahian0Hossein Masoomi1دانشجوی سابق کارشناسی ارشد، بخش گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید باهنر کرماندانشیار بخش گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان<em>Tomato aspermy virus</em> (TAV) has a wide host range and usually infects ornamental plants. To identify and characterize molecular and biological properties of the virus, 436 samples were collected from various provinces of Iran. TAV was detected in three samples of ornamental plants through double antibody sandwich ELISA test using specific polyclonal antibody. The 687 bp segment including the coat protein (CP) gene was amplified by RT-PCR, cloned and sequenced. Nucleotide sequences of the CP gene of Iranian TAV isolates were compared with available GenBank isolates. Phylogenetic analysis showed that TAV isolates were classified into two groups I and II, and the group II was divided in two subgroups IIA and IIB. All the Iranian TAV isolates were classified in subgroup IIA and shared the high nucleotide and amino acid sequence identities (more than 99%). The results of this study also showed a wide host range of the Iranian TAV isolates among test plants. This study is the first report of TAV occurrence in Iran and the first natural occurrence of TAV on <em>Petunia hybrida</em> in the world.https://jab.uk.ac.ir/article_468_1583d6c0780a7f7705dba14d30855150.pdftomato aspermy virusphylogenetic positionrt-pcr |
| spellingShingle | Mohammad Madahian Hossein Masoomi Partial biological and molecular characterization of Iranian isolates of Tomato aspermy virus مجله بیوتکنولوژی کشاورزی tomato aspermy virus phylogenetic position rt-pcr |
| title | Partial biological and molecular characterization of Iranian isolates of Tomato aspermy virus |
| title_full | Partial biological and molecular characterization of Iranian isolates of Tomato aspermy virus |
| title_fullStr | Partial biological and molecular characterization of Iranian isolates of Tomato aspermy virus |
| title_full_unstemmed | Partial biological and molecular characterization of Iranian isolates of Tomato aspermy virus |
| title_short | Partial biological and molecular characterization of Iranian isolates of Tomato aspermy virus |
| title_sort | partial biological and molecular characterization of iranian isolates of tomato aspermy virus |
| topic | tomato aspermy virus phylogenetic position rt-pcr |
| url | https://jab.uk.ac.ir/article_468_1583d6c0780a7f7705dba14d30855150.pdf |
| work_keys_str_mv | AT mohammadmadahian partialbiologicalandmolecularcharacterizationofiranianisolatesoftomatoaspermyvirus AT hosseinmasoomi partialbiologicalandmolecularcharacterizationofiranianisolatesoftomatoaspermyvirus |