Quality assessment, HPTLC-DPPH, analytical quality by design based HPTLC method development for estimation of piperine in piper species and marketed formulations

Background: Quality assessment of herbs is an essential aspect. In this study HPTLC method is developed by employing the quality based optimization approach with the aid of analytical quality by design tools and the quality assessment of the Piper species. Result: The Quality assessment was carried...

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Main Authors: Amruta Balekundri, Pramod J Hurkadale, Harsha Hegde
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:Green Analytical Chemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2772577424000028
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author Amruta Balekundri
Pramod J Hurkadale
Harsha Hegde
author_facet Amruta Balekundri
Pramod J Hurkadale
Harsha Hegde
author_sort Amruta Balekundri
collection DOAJ
description Background: Quality assessment of herbs is an essential aspect. In this study HPTLC method is developed by employing the quality based optimization approach with the aid of analytical quality by design tools and the quality assessment of the Piper species. Result: The Quality assessment was carried out for the piper species which was under the acceptance criteria of Ayurvedic Pharmacopeia of India. Eco-friendly and Quality based HPTLC method was developed and validated for Piperine in Extracts as well as marketed formulation. Further the antioxidant potential was checked by the HPTLC-DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Conclusion: Quality assessed extracts and Analytical quality based HPTLC method which was eco-friendly, simple and reliable was developed and validated.
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spelling doaj.art-b8685d2ad3a24bbe9703f8cf8f9a00352024-03-14T06:17:11ZengElsevierGreen Analytical Chemistry2772-57742024-03-018100093Quality assessment, HPTLC-DPPH, analytical quality by design based HPTLC method development for estimation of piperine in piper species and marketed formulationsAmruta Balekundri0Pramod J Hurkadale1Harsha Hegde2Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, India; KLE Academy of Higher Education and Research (KAHER), Belagavi-590010, Karnataka, India; Corresponding authors.Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, India; KLE Academy of Higher Education and Research (KAHER), Belagavi-590010, Karnataka, India; Corresponding authors.ICMR-National Institute of Traditional Medicine, Belagavi-590010, Karnataka, IndiaBackground: Quality assessment of herbs is an essential aspect. In this study HPTLC method is developed by employing the quality based optimization approach with the aid of analytical quality by design tools and the quality assessment of the Piper species. Result: The Quality assessment was carried out for the piper species which was under the acceptance criteria of Ayurvedic Pharmacopeia of India. Eco-friendly and Quality based HPTLC method was developed and validated for Piperine in Extracts as well as marketed formulation. Further the antioxidant potential was checked by the HPTLC-DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Conclusion: Quality assessed extracts and Analytical quality based HPTLC method which was eco-friendly, simple and reliable was developed and validated.http://www.sciencedirect.com/science/article/pii/S2772577424000028Quality assessmentHPTLCHPTLC-DPPHAQbDAGREE scale
spellingShingle Amruta Balekundri
Pramod J Hurkadale
Harsha Hegde
Quality assessment, HPTLC-DPPH, analytical quality by design based HPTLC method development for estimation of piperine in piper species and marketed formulations
Green Analytical Chemistry
Quality assessment
HPTLC
HPTLC-DPPH
AQbD
AGREE scale
title Quality assessment, HPTLC-DPPH, analytical quality by design based HPTLC method development for estimation of piperine in piper species and marketed formulations
title_full Quality assessment, HPTLC-DPPH, analytical quality by design based HPTLC method development for estimation of piperine in piper species and marketed formulations
title_fullStr Quality assessment, HPTLC-DPPH, analytical quality by design based HPTLC method development for estimation of piperine in piper species and marketed formulations
title_full_unstemmed Quality assessment, HPTLC-DPPH, analytical quality by design based HPTLC method development for estimation of piperine in piper species and marketed formulations
title_short Quality assessment, HPTLC-DPPH, analytical quality by design based HPTLC method development for estimation of piperine in piper species and marketed formulations
title_sort quality assessment hptlc dpph analytical quality by design based hptlc method development for estimation of piperine in piper species and marketed formulations
topic Quality assessment
HPTLC
HPTLC-DPPH
AQbD
AGREE scale
url http://www.sciencedirect.com/science/article/pii/S2772577424000028
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AT pramodjhurkadale qualityassessmenthptlcdpphanalyticalqualitybydesignbasedhptlcmethoddevelopmentforestimationofpiperineinpiperspeciesandmarketedformulations
AT harshahegde qualityassessmenthptlcdpphanalyticalqualitybydesignbasedhptlcmethoddevelopmentforestimationofpiperineinpiperspeciesandmarketedformulations