Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture
We investigated the anther culture (AC) efficiency of thirteen F4 combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. T...
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Czech Academy of Agricultural Sciences
2020-12-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
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| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-202004-0004_generation-of-doubled-haploid-lines-from-winter-wheat-triticum-aestivum-l-breeding-material-using-in-vitro-a.php |
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| author | Osama Zuhair Kanbar Csaba Lantos Paul Karumba Chege Erzsebet Kiss Janos Pauk |
| author_facet | Osama Zuhair Kanbar Csaba Lantos Paul Karumba Chege Erzsebet Kiss Janos Pauk |
| author_sort | Osama Zuhair Kanbar |
| collection | DOAJ |
| description | We investigated the anther culture (AC) efficiency of thirteen F4 combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25-87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application. |
| first_indexed | 2024-04-10T08:28:44Z |
| format | Article |
| id | doaj.art-b86a15933d584b0fb453517a20f2acc9 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:28:44Z |
| publishDate | 2020-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-b86a15933d584b0fb453517a20f2acc92023-02-23T03:30:50ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252020-12-0156415015810.17221/113/2019-CJGPBcjg-202004-0004Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther cultureOsama Zuhair Kanbar0Csaba Lantos1Paul Karumba Chege2Erzsebet Kiss3Janos Pauk4Doctoral School of Plant Science, Szent Istvan University, Gödöllő, HungaryDepartemant of Biotechnology, Cereal Research Non-Profit Ltd., Szeged, HungaryDoctoral School of Plant Science, Szent Istvan University, Gödöllő, HungaryGenetics, Microbiology and Biotechnology Institute, Szent Istvan University, Gödöllő, HungaryDepartemant of Biotechnology, Cereal Research Non-Profit Ltd., Szeged, HungaryWe investigated the anther culture (AC) efficiency of thirteen F4 combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25-87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.https://cjgpb.agriculturejournals.cz/artkey/cjg-202004-0004_generation-of-doubled-haploid-lines-from-winter-wheat-triticum-aestivum-l-breeding-material-using-in-vitro-a.phpbreedinghaploidtriticum aestivum l.wheat |
| spellingShingle | Osama Zuhair Kanbar Csaba Lantos Paul Karumba Chege Erzsebet Kiss Janos Pauk Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture Czech Journal of Genetics and Plant Breeding breeding haploid triticum aestivum l. wheat |
| title | Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture |
| title_full | Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture |
| title_fullStr | Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture |
| title_full_unstemmed | Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture |
| title_short | Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture |
| title_sort | generation of doubled haploid lines from winter wheat triticum aestivum l breeding material using in vitro anther culture |
| topic | breeding haploid triticum aestivum l. wheat |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-202004-0004_generation-of-doubled-haploid-lines-from-winter-wheat-triticum-aestivum-l-breeding-material-using-in-vitro-a.php |
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