Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis
Optimization of total flavonoid compound (TFC) extraction from Gynura medica leaf was investigated using response surface methodology (RSM) in this paper. The conditions investigated were 30–60% (v/v) ethanol concentration (X1), 85–95 °C extraction temperature (X2) and 30–50 (v/w) liquid-to-solid ra...
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MDPI AG
2010-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/11/11/4750/ |
| _version_ | 1811297210535510016 |
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| author | Shuwen Cao Ruzhen Yang Chunpeng Wan Wei Liu Yanying Yu Binbin Xu |
| author_facet | Shuwen Cao Ruzhen Yang Chunpeng Wan Wei Liu Yanying Yu Binbin Xu |
| author_sort | Shuwen Cao |
| collection | DOAJ |
| description | Optimization of total flavonoid compound (TFC) extraction from Gynura medica leaf was investigated using response surface methodology (RSM) in this paper. The conditions investigated were 30–60% (v/v) ethanol concentration (X1), 85–95 °C extraction temperature (X2) and 30–50 (v/w) liquid-to-solid ratio (X3). Statistical analysis of the experiments indicated that temperature and liquid-to-solid ratio significantly affected TFC extraction (p < 0.01). The Box-Behnken experiment design showed that polynomial regression models were in good agreement with the experimental results, with the coefficients of determination of 0.9325 for TFC yield. The optimal conditions for maximum TFC yield were 55% ethanol, 92 °C and 50 (v/w) liquid-to-solid ratio with a 30 min extraction time. Extracts from these conditions showed a moderate antioxidant value of 54.78 μmol quercetin/g dry material (DM), 137.3 μmol trolox/g DM for 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 108.21 μmol quercetin/g DM, 242.31 μmol trolox/g DM for 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+), respectively. HPLC-DAD-MS analysis showed that kaempferol-3-O-glucoside was the principal flavonoid compound in Gynura medica leaf. |
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| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T06:00:50Z |
| publishDate | 2010-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b86c23f97b824959a9cd8e14cef579952022-12-22T02:59:27ZengMDPI AGInternational Journal of Molecular Sciences1422-00672010-11-0111114750476310.3390/ijms11114750Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition AnalysisShuwen CaoRuzhen YangChunpeng WanWei LiuYanying YuBinbin XuOptimization of total flavonoid compound (TFC) extraction from Gynura medica leaf was investigated using response surface methodology (RSM) in this paper. The conditions investigated were 30–60% (v/v) ethanol concentration (X1), 85–95 °C extraction temperature (X2) and 30–50 (v/w) liquid-to-solid ratio (X3). Statistical analysis of the experiments indicated that temperature and liquid-to-solid ratio significantly affected TFC extraction (p < 0.01). The Box-Behnken experiment design showed that polynomial regression models were in good agreement with the experimental results, with the coefficients of determination of 0.9325 for TFC yield. The optimal conditions for maximum TFC yield were 55% ethanol, 92 °C and 50 (v/w) liquid-to-solid ratio with a 30 min extraction time. Extracts from these conditions showed a moderate antioxidant value of 54.78 μmol quercetin/g dry material (DM), 137.3 μmol trolox/g DM for 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 108.21 μmol quercetin/g DM, 242.31 μmol trolox/g DM for 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+), respectively. HPLC-DAD-MS analysis showed that kaempferol-3-O-glucoside was the principal flavonoid compound in Gynura medica leaf.http://www.mdpi.com/1422-0067/11/11/4750/Gynura medicaresponse surface methodologyflavonoid compoundsantioxidant activity |
| spellingShingle | Shuwen Cao Ruzhen Yang Chunpeng Wan Wei Liu Yanying Yu Binbin Xu Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis International Journal of Molecular Sciences Gynura medica response surface methodology flavonoid compounds antioxidant activity |
| title | Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis |
| title_full | Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis |
| title_fullStr | Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis |
| title_full_unstemmed | Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis |
| title_short | Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis |
| title_sort | optimization of total flavonoid compound extraction from gynura medica leaf using response surface methodology and chemical composition analysis |
| topic | Gynura medica response surface methodology flavonoid compounds antioxidant activity |
| url | http://www.mdpi.com/1422-0067/11/11/4750/ |
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