Characterization and functional biology of the soybean aleurone layer
Abstract Background Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean’s genes still remains to be investigate...
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-018-1579-8 |
| _version_ | 1818911797688664064 |
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| author | Monica A. Schmidt Eliot M. Herman |
| author_facet | Monica A. Schmidt Eliot M. Herman |
| author_sort | Monica A. Schmidt |
| collection | DOAJ |
| description | Abstract Background Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean’s genes still remains to be investigated. A major hurdle in the functional genomics of soybean is a rapid method to test gene constructs before embarking on stable transformation experiments. Results In this paper we describe the morphology and composition of the persistent single-cell aleurone layer that derives from the endosperm of developing soybean seeds. Its composition compared to cotyledonary tissue indicates the aleurone layer plays a role in both abiotic and biotic stress. The potential utility as the aleurone layer as a transient expression system in soybean was shown. As a near transparent single-cell layer it can be used as a transient expression system to study transgene expression and inter- and intra-cellular targeting as it is amenable to microscopic techniques. Conclusion The transparent single cell aleurone layer was shown to be compositionally comparable to cotyledonary tissue in soybean with an enrichment in oxidative response proteins and shown to be a potential transient expression platform. |
| first_indexed | 2024-12-19T23:04:25Z |
| format | Article |
| id | doaj.art-b86e2c374f0b4bf0a4197252a53cd252 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-19T23:04:25Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-b86e2c374f0b4bf0a4197252a53cd2522022-12-21T20:02:26ZengBMCBMC Plant Biology1471-22292018-12-011811910.1186/s12870-018-1579-8Characterization and functional biology of the soybean aleurone layerMonica A. Schmidt0Eliot M. Herman1School of Plant Sciences/BIO5 Institute/University of ArizonaSchool of Plant Sciences/BIO5 Institute/University of ArizonaAbstract Background Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean’s genes still remains to be investigated. A major hurdle in the functional genomics of soybean is a rapid method to test gene constructs before embarking on stable transformation experiments. Results In this paper we describe the morphology and composition of the persistent single-cell aleurone layer that derives from the endosperm of developing soybean seeds. Its composition compared to cotyledonary tissue indicates the aleurone layer plays a role in both abiotic and biotic stress. The potential utility as the aleurone layer as a transient expression system in soybean was shown. As a near transparent single-cell layer it can be used as a transient expression system to study transgene expression and inter- and intra-cellular targeting as it is amenable to microscopic techniques. Conclusion The transparent single cell aleurone layer was shown to be compositionally comparable to cotyledonary tissue in soybean with an enrichment in oxidative response proteins and shown to be a potential transient expression platform.http://link.springer.com/article/10.1186/s12870-018-1579-8AleuroneTransient expressionSoybeanSeed-specificSub-cellular localization |
| spellingShingle | Monica A. Schmidt Eliot M. Herman Characterization and functional biology of the soybean aleurone layer BMC Plant Biology Aleurone Transient expression Soybean Seed-specific Sub-cellular localization |
| title | Characterization and functional biology of the soybean aleurone layer |
| title_full | Characterization and functional biology of the soybean aleurone layer |
| title_fullStr | Characterization and functional biology of the soybean aleurone layer |
| title_full_unstemmed | Characterization and functional biology of the soybean aleurone layer |
| title_short | Characterization and functional biology of the soybean aleurone layer |
| title_sort | characterization and functional biology of the soybean aleurone layer |
| topic | Aleurone Transient expression Soybean Seed-specific Sub-cellular localization |
| url | http://link.springer.com/article/10.1186/s12870-018-1579-8 |
| work_keys_str_mv | AT monicaaschmidt characterizationandfunctionalbiologyofthesoybeanaleuronelayer AT eliotmherman characterizationandfunctionalbiologyofthesoybeanaleuronelayer |