Analysis of oral microbiome on temporary anchorage devices under different periodontal conditions
Abstract Background Temporary anchorage devices (TADs) are maximum anchorages that have been widely used in orthodontic treatment. The aim of the study was to uncover whether a history of periodontitis would influence microbiome colonization on the TAD surface. Results Patients were grouped by perio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-10-01
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| Series: | Progress in Orthodontics |
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| Online Access: | https://doi.org/10.1186/s40510-023-00488-x |
| _version_ | 1797451358587060224 |
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| author | Ningrui Zhao Qian Zhang Yanning Guo Shengjie Cui Yajing Tian Yanheng Zhou Xuedong Wang |
| author_facet | Ningrui Zhao Qian Zhang Yanning Guo Shengjie Cui Yajing Tian Yanheng Zhou Xuedong Wang |
| author_sort | Ningrui Zhao |
| collection | DOAJ |
| description | Abstract Background Temporary anchorage devices (TADs) are maximum anchorages that have been widely used in orthodontic treatment. The aim of the study was to uncover whether a history of periodontitis would influence microbiome colonization on the TAD surface. Results Patients were grouped by periodontal evaluations before the orthodontic treatment. Patients with healthy periodontal conditions were classified as the healthy group, and patients diagnosed with periodontitis stage II or even worse were classified as the periodontitis group. Scanning electron microscopy (SEM) was used to analyze the existence of biofilm on the surface of 4 TADs from the healthy group and 4 TADs from the periodontitis group. Fifteen TADs from the healthy group and 12 TADs from the periodontitis group were collected. The microorganisms on the surface of TADs were harvested and analyzed by 16S rRNA gene sequencing. α-diversity indices and β-diversity indices were calculated. Wilcoxon’s test was used to determine differences between genera, species as well as KEGG functions. SEM analysis revealed bacteria colonization on the surface of TADs from both groups. Principal coordinate analysis (PCoA) based on β diversity revealed differential sample clusters depending on periodontal conditions (P < 0.01). When comparing specific genera, Fusobacterium, Porphyromonas, Saccharibacteria_(TM7)_[G-1], Dialister, Parvimonas, Fretibacterium, Treponema were more enriched in TADs in the periodontitis group. In the KEGG analysis, TADs in the periodontitis group demonstrated enriched microbial activities involved with translation, genetic information processing, metabolism, and cell motility. Conclusions This analysis elucidated the difference in total composition and function of TADs oral microorganisms between patients periodontally healthy and with periodontitis. |
| first_indexed | 2024-03-09T14:52:35Z |
| format | Article |
| id | doaj.art-b87011268aad4fad888a0cdd0fdbd840 |
| institution | Directory Open Access Journal |
| issn | 2196-1042 |
| language | English |
| last_indexed | 2024-03-09T14:52:35Z |
| publishDate | 2023-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Progress in Orthodontics |
| spelling | doaj.art-b87011268aad4fad888a0cdd0fdbd8402023-11-26T14:22:38ZengSpringerOpenProgress in Orthodontics2196-10422023-10-0124111010.1186/s40510-023-00488-xAnalysis of oral microbiome on temporary anchorage devices under different periodontal conditionsNingrui Zhao0Qian Zhang1Yanning Guo2Shengjie Cui3Yajing Tian4Yanheng Zhou5Xuedong Wang6Department of Orthodontics, Peking University School and Hospital of StomatologyCentral Laboratory, Peking University School and Hospital of Stomatology, Haidian DistrictDepartment of Orthodontics, Peking University School and Hospital of StomatologyDepartment of Orthodontics, Peking University School and Hospital of StomatologyDepartment of Orthodontics, Peking University School and Hospital of StomatologyDepartment of Orthodontics, Peking University School and Hospital of StomatologyDepartment of Orthodontics, Peking University School and Hospital of StomatologyAbstract Background Temporary anchorage devices (TADs) are maximum anchorages that have been widely used in orthodontic treatment. The aim of the study was to uncover whether a history of periodontitis would influence microbiome colonization on the TAD surface. Results Patients were grouped by periodontal evaluations before the orthodontic treatment. Patients with healthy periodontal conditions were classified as the healthy group, and patients diagnosed with periodontitis stage II or even worse were classified as the periodontitis group. Scanning electron microscopy (SEM) was used to analyze the existence of biofilm on the surface of 4 TADs from the healthy group and 4 TADs from the periodontitis group. Fifteen TADs from the healthy group and 12 TADs from the periodontitis group were collected. The microorganisms on the surface of TADs were harvested and analyzed by 16S rRNA gene sequencing. α-diversity indices and β-diversity indices were calculated. Wilcoxon’s test was used to determine differences between genera, species as well as KEGG functions. SEM analysis revealed bacteria colonization on the surface of TADs from both groups. Principal coordinate analysis (PCoA) based on β diversity revealed differential sample clusters depending on periodontal conditions (P < 0.01). When comparing specific genera, Fusobacterium, Porphyromonas, Saccharibacteria_(TM7)_[G-1], Dialister, Parvimonas, Fretibacterium, Treponema were more enriched in TADs in the periodontitis group. In the KEGG analysis, TADs in the periodontitis group demonstrated enriched microbial activities involved with translation, genetic information processing, metabolism, and cell motility. Conclusions This analysis elucidated the difference in total composition and function of TADs oral microorganisms between patients periodontally healthy and with periodontitis.https://doi.org/10.1186/s40510-023-00488-xTemporary anchorage devicesPeriodontitisMicrobiomeOrthodontic treatment |
| spellingShingle | Ningrui Zhao Qian Zhang Yanning Guo Shengjie Cui Yajing Tian Yanheng Zhou Xuedong Wang Analysis of oral microbiome on temporary anchorage devices under different periodontal conditions Progress in Orthodontics Temporary anchorage devices Periodontitis Microbiome Orthodontic treatment |
| title | Analysis of oral microbiome on temporary anchorage devices under different periodontal conditions |
| title_full | Analysis of oral microbiome on temporary anchorage devices under different periodontal conditions |
| title_fullStr | Analysis of oral microbiome on temporary anchorage devices under different periodontal conditions |
| title_full_unstemmed | Analysis of oral microbiome on temporary anchorage devices under different periodontal conditions |
| title_short | Analysis of oral microbiome on temporary anchorage devices under different periodontal conditions |
| title_sort | analysis of oral microbiome on temporary anchorage devices under different periodontal conditions |
| topic | Temporary anchorage devices Periodontitis Microbiome Orthodontic treatment |
| url | https://doi.org/10.1186/s40510-023-00488-x |
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