Lactobacillus casei CCFM1074 Alleviates Collagen-Induced Arthritis in Rats via Balancing Treg/Th17 and Modulating the Metabolites and Gut Microbiota
Gut microbiota and their influence on metabolites are receiving increasing attentions in autoimmune diseases including rheumatoid arthritis (RA). Probiotics become a promising manipulator to prevent or attenuate the progression of arthritis, some evidences suggesting that lactobacilli treatment infl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.680073/full |
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| author | Zhexin Fan Zhexin Fan R. Paul Ross R. Paul Ross Catherine Stanton Catherine Stanton Bao Hou Jianxin Zhao Jianxin Zhao Jianxin Zhao Hao Zhang Hao Zhang Hao Zhang Hao Zhang Bo Yang Bo Yang Bo Yang Wei Chen Wei Chen Wei Chen |
| author_facet | Zhexin Fan Zhexin Fan R. Paul Ross R. Paul Ross Catherine Stanton Catherine Stanton Bao Hou Jianxin Zhao Jianxin Zhao Jianxin Zhao Hao Zhang Hao Zhang Hao Zhang Hao Zhang Bo Yang Bo Yang Bo Yang Wei Chen Wei Chen Wei Chen |
| author_sort | Zhexin Fan |
| collection | DOAJ |
| description | Gut microbiota and their influence on metabolites are receiving increasing attentions in autoimmune diseases including rheumatoid arthritis (RA). Probiotics become a promising manipulator to prevent or attenuate the progression of arthritis, some evidences suggesting that lactobacilli treatment influence the responses to RA therapy but the underlying mechanisms are limited. By using a collagen-induced arthritis (CIA) rats, the study assessed the effects of two L. casei strains (CCFM1074, CCFM1075) on the immune responses, gut microbiota and plasma metabolites via an integrated cross-omics approach including fecal 16S rRNA high-throughput sequencing and plasma metabolomics. The genome of the two strains was analyzed and compared using whole-genome sequencing approach to further confirm biology functions. CCFM1074 reduced arthritic symptoms while CCFM1075 did not, though both strains down-regulated the plasma IL-6 and Th17 cells proportion. CCFM1074 enhanced the proportion of Treg cells in mesenteric lymph nodes which was significantly associated with SCFAs upregulation, as well as with genomic evidence that CCFM1074 possesses more functional genes involved in carbohydrate metabolism. Moreover, CCFM1074 regulated the gut microbiota, including modulating community structure, decreasing the abundance of Alistipes and Parabacteroides and increasing the abundance of Oscillibacter. The differential metabolites modulated by CCFM1074 including eicosapentaenoic acid and docosapentaenoic acid which involved in unsaturated fatty acids metabolism. Furthermore, alterations of gut microbial community were correlated with the plasma metabolome. In summary, L. casei CCFM1074 alleviated arthritis via rebalancing gut microbiota, immune responses and plasma metabolites. |
| first_indexed | 2024-12-20T01:28:44Z |
| format | Article |
| id | doaj.art-b8703d4fdaf945088cd1e3bc2497e137 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T01:28:44Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-b8703d4fdaf945088cd1e3bc2497e1372022-12-21T19:58:10ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-05-011210.3389/fimmu.2021.680073680073Lactobacillus casei CCFM1074 Alleviates Collagen-Induced Arthritis in Rats via Balancing Treg/Th17 and Modulating the Metabolites and Gut MicrobiotaZhexin Fan0Zhexin Fan1R. Paul Ross2R. Paul Ross3Catherine Stanton4Catherine Stanton5Bao Hou6Jianxin Zhao7Jianxin Zhao8Jianxin Zhao9Hao Zhang10Hao Zhang11Hao Zhang12Hao Zhang13Bo Yang14Bo Yang15Bo Yang16Wei Chen17Wei Chen18Wei Chen19State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaInternational Joint Research Laboratory for Pharmabiotics & Antibiotic Resistance, Jiangnan University, Wuxi, ChinaAPC Microbiome Ireland, University College Cork, Cork, IrelandInternational Joint Research Laboratory for Pharmabiotics & Antibiotic Resistance, Jiangnan University, Wuxi, ChinaTeagasc Food Research Centre, Moorepark, Co., Cork, IrelandDepartment of Basic Medicine, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaNational Engineering Research Center for Functional Food, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaNational Engineering Research Center for Functional Food, Jiangnan University, Wuxi, ChinaWuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaInternational Joint Research Laboratory for Pharmabiotics & Antibiotic Resistance, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaNational Engineering Research Center for Functional Food, Jiangnan University, Wuxi, ChinaGut microbiota and their influence on metabolites are receiving increasing attentions in autoimmune diseases including rheumatoid arthritis (RA). Probiotics become a promising manipulator to prevent or attenuate the progression of arthritis, some evidences suggesting that lactobacilli treatment influence the responses to RA therapy but the underlying mechanisms are limited. By using a collagen-induced arthritis (CIA) rats, the study assessed the effects of two L. casei strains (CCFM1074, CCFM1075) on the immune responses, gut microbiota and plasma metabolites via an integrated cross-omics approach including fecal 16S rRNA high-throughput sequencing and plasma metabolomics. The genome of the two strains was analyzed and compared using whole-genome sequencing approach to further confirm biology functions. CCFM1074 reduced arthritic symptoms while CCFM1075 did not, though both strains down-regulated the plasma IL-6 and Th17 cells proportion. CCFM1074 enhanced the proportion of Treg cells in mesenteric lymph nodes which was significantly associated with SCFAs upregulation, as well as with genomic evidence that CCFM1074 possesses more functional genes involved in carbohydrate metabolism. Moreover, CCFM1074 regulated the gut microbiota, including modulating community structure, decreasing the abundance of Alistipes and Parabacteroides and increasing the abundance of Oscillibacter. The differential metabolites modulated by CCFM1074 including eicosapentaenoic acid and docosapentaenoic acid which involved in unsaturated fatty acids metabolism. Furthermore, alterations of gut microbial community were correlated with the plasma metabolome. In summary, L. casei CCFM1074 alleviated arthritis via rebalancing gut microbiota, immune responses and plasma metabolites.https://www.frontiersin.org/articles/10.3389/fimmu.2021.680073/fullLactobacillus caseiarthritisimmunity responsesgut microbiotaserum metabolomegenome |
| spellingShingle | Zhexin Fan Zhexin Fan R. Paul Ross R. Paul Ross Catherine Stanton Catherine Stanton Bao Hou Jianxin Zhao Jianxin Zhao Jianxin Zhao Hao Zhang Hao Zhang Hao Zhang Hao Zhang Bo Yang Bo Yang Bo Yang Wei Chen Wei Chen Wei Chen Lactobacillus casei CCFM1074 Alleviates Collagen-Induced Arthritis in Rats via Balancing Treg/Th17 and Modulating the Metabolites and Gut Microbiota Frontiers in Immunology Lactobacillus casei arthritis immunity responses gut microbiota serum metabolome genome |
| title | Lactobacillus casei CCFM1074 Alleviates Collagen-Induced Arthritis in Rats via Balancing Treg/Th17 and Modulating the Metabolites and Gut Microbiota |
| title_full | Lactobacillus casei CCFM1074 Alleviates Collagen-Induced Arthritis in Rats via Balancing Treg/Th17 and Modulating the Metabolites and Gut Microbiota |
| title_fullStr | Lactobacillus casei CCFM1074 Alleviates Collagen-Induced Arthritis in Rats via Balancing Treg/Th17 and Modulating the Metabolites and Gut Microbiota |
| title_full_unstemmed | Lactobacillus casei CCFM1074 Alleviates Collagen-Induced Arthritis in Rats via Balancing Treg/Th17 and Modulating the Metabolites and Gut Microbiota |
| title_short | Lactobacillus casei CCFM1074 Alleviates Collagen-Induced Arthritis in Rats via Balancing Treg/Th17 and Modulating the Metabolites and Gut Microbiota |
| title_sort | lactobacillus casei ccfm1074 alleviates collagen induced arthritis in rats via balancing treg th17 and modulating the metabolites and gut microbiota |
| topic | Lactobacillus casei arthritis immunity responses gut microbiota serum metabolome genome |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.680073/full |
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